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Dyskerin (DKC1) Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS854423

MBS854423 is an antibody targeting DKC1. Also known as Dyskerin Antibody. Reactive in Human, Mouse, Rat. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Dyskerin (DKC1), Antibody
Full Product Name
Dyskerin Antibody
Product Gene Name
Product Synonym Names
CBF5 homolog; DKC1; EC 5.4.99.-; Nucleolar protein NAP57
Species Reactivity
Human, Mouse, Rat
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
Dyskerin antibody detects endogenous levels of total Dyskerin protein.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
47,603 Da
OMIM
300126
3D Structure
ModBase 3D Structure for O60832
Immunogen
The antiserum was produced against synthesized peptide derived from Internal of human Dyskerin.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-DKC1 antibody
Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-DKC1 antibody
WB: 1:500~1:1000
IF: 1:100~1:500
ELISA: 1:10000

Testing Data of anti-DKC1 antibody

Anti-DKC1 Antibody – Cat# MBS854423

Western Blot (WB) of anti-DKC1 antibody

Peptide-+ Western blot analysis of extracts from HeLa cells, using Dyskerin antibody. Immunofluorescence analysis of HeLa cells, using Dyskerin antibody.
Anti-DKC1 Antibody, Western Blot (WB) – Cat# MBS854423

Product References and Citations for anti-DKC1 antibody

Heiss N.S., Nat. Genet. 19:32-38(1998). Knight S.W., Am. J. Hum. Genet. 65:50-58(1999). Hassock S., Genomics 55:21-27(1999).

Other Notes

Small volumes of anti-DKC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DKC1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
H/ACA ribonucleoprotein complex subunit 4 isoform 2
NCBI Official Synonym Full Names
dyskerin pseudouridine synthase 1
NCBI Official Symbol
DKC1
NCBI Official Synonym Symbols
DKC; CBF5; DKCX; NAP57; NOLA4; XAP101
UniProt Protein Name
H/ACA ribonucleoprotein complex subunit 4
UniProt Synonym Protein Names
CBF5 homolog; Dyskerin; Nopp140-associated protein of 57 kDa; Nucleolar protein NAP57; Nucleolar protein family A member 4; snoRNP protein DKC1
UniProt Gene Name
DKC1
UniProt Synonym Gene Names
NOLA4
NCBI Protein Information
H/ACA ribonucleoprotein complex subunit 4

NCBI Summary for DKC1

This gene functions in two distinct complexes. It plays an active role in telomerase stabilization and maintenance, as well as recognition of snoRNAs containing H/ACA sequences which provides stability during biogenesis and assembly into H/ACA small nucleolar RNA ribonucleoproteins (snoRNPs). This gene is highly conserved and widely expressed, and may play additional roles in nucleo-cytoplasmic shuttling, DNA damage response, and cell adhesion. Mutations have been associated with X-linked dyskeratosis congenita. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
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UniProt Comments for DKC1

Isoform 1: Required for ribosome biogenesis and telomere maintenance. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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