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ARFGAP1 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS854458

MBS854458 is an antibody targeting ARFGAP1. Also known as ARFGAP1 Antibody. Reactive in Human, Mouse, Rat. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ARFGAP1, Antibody
Full Product Name
ARFGAP1 Antibody
Product Gene Name
Product Synonym Names
ADP-ribosylation factor 1 GTPase activating protein; ADP-ribosylation factor GTPase activating protein 1; ARF1 GAP; ARF1-directed GTPase-activating protein; ARF1GAP; ARFG1; ARFGAP1; ARG1; GAP protein
Species Reactivity
Human, Mouse, Rat
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
ARFGAP1 antibody detects endogenous levels of total ARFGAP1 protein.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
39,705 Da
OMIM
608377
3D Structure
ModBase 3D Structure for Q8N6T3
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human ARFGAP1.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-ARFGAP1 antibody
Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-ARFGAP1 antibody
WB: 1:500~1:1000
IF: 1:100~1:500
ELISA: 1:000

Testing Data of anti-ARFGAP1 antibody

Anti-ARFGAP1 Antibody – Cat# MBS854458

Western Blot (WB) of anti-ARFGAP1 antibody

Peptide-+ Western blot analysis of extracts from HeLa cells, MCF-7 cells and COLO cells, using ARFGAP1 antibody. Immunofluorescence analysis of MCF-7 cells, using ARFGAP1 antibody.
Anti-ARFGAP1 Antibody, Western Blot (WB) – Cat# MBS854458

Product References and Citations for anti-ARFGAP1 antibody

Ota T., Nat. Genet. 36:40-45(2004). Deloukas P., Nature 414:865-871(2001). The MGC Project Team; Genome Res. 14:2121-2127(2004).

Other Notes

Small volumes of anti-ARFGAP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ARFGAP1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ADP-ribosylation factor GTPase-activating protein 1 isoform c
NCBI Official Synonym Full Names
ADP ribosylation factor GTPase activating protein 1
NCBI Official Symbol
ARFGAP1
NCBI Official Synonym Symbols
ARF1GAP; HRIHFB2281
UniProt Protein Name
ADP-ribosylation factor GTPase-activating protein 1
UniProt Synonym Protein Names
ADP-ribosylation factor 1 GTPase-activating protein; ARF1 GAP; ARF1-directed GTPase-activating protein
UniProt Gene Name
ARFGAP1
UniProt Synonym Gene Names
ARF1GAP; ARF GAP 1; ARF1 GAP
NCBI Protein Information
ADP-ribosylation factor GTPase-activating protein 1

NCBI Summary for ARFGAP1

The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
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UniProt Comments for ARFGAP1

GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ARFGAP1 antibody

 Reference Product  PubMed Publications
 ARF1 antibody  >32 publications with ARFGAP1 and ARF1
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