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MAP3K1 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS854512

MBS854512 is an antibody targeting MAP3K1. Also known as MAP3K1 (Phospho-Thr1402) Antibody. Reactive in Human, Mouse, Rat. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MAP3K1, Antibody
Full Product Name
MAP3K1 (Phospho-Thr1402) Antibody
Product Gene Name
Product Synonym Names
EC 2.7.11.25; kinase MEKK1; M3K1; MAP3K1; MAPK/ERK kinase kinase 1; MAPKKK1; MEK kinase 1; MEKK; MEKK 1; MEKK1; mitogen-activated protein kinase kinase kinase 1
Species Reactivity
Human, Mouse, Rat
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificity
MAP3K1 (Phospho-Thr1402) antibody detects endogenous levels of MAP3K1 only when phosphorylated at threonine 1402.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
164,470 Da
OMIM
600982
3D Structure
ModBase 3D Structure for Q13233
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human MAP3K1 around the phosphorylation site of threonine 1402 (K-G-TP-G-A).
All Sites
Human: Thr1402
Mouse: Thr1381
Rat: Thr1381
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-MAP3K1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-MAP3K1 antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:40000

Testing Data of anti-MAP3K1 antibody

P-peptide-+
Anti-MAP3K1 Antibody – Cat# MBS854512

Immunohistochemistry (IHC) of anti-MAP3K1 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue using MAP3K1 (Phospho-Thr1402) antibody. Western blot analysis of extracts from Jurkat cells, using MAP3K1 (Phospho-Thr1402) antibody.
Anti-MAP3K1 Antibody, Immunohistochemistry (IHC) – Cat# MBS854512

Product References and Citations for anti-MAP3K1 antibody

Schmutz J., Nature 431:268-274(2004). Xia Y., Genes Dev. 12:3369-3381(1998). Vinik B.S., Mamm. Genome 6:782-783(1995).

Other Notes

Small volumes of anti-MAP3K1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MAP3K1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase 1
NCBI Official Symbol
MAP3K1
NCBI Official Synonym Symbols
MEKK; MEKK1; SRXY6; MEKK 1; MAPKKK1
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase 1
UniProt Synonym Protein Names
MAPK/ERK kinase kinase 1; MEK kinase 1; MEKK 1
UniProt Gene Name
MAP3K1
UniProt Synonym Gene Names
MAPKKK1; MEKK; MEKK1; MEK kinase 1; MEKK 1
NCBI Protein Information
mitogen-activated protein kinase kinase kinase 1

NCBI Summary for MAP3K1

The protein encoded by this gene is a serine/threonine kinase and is part of some signal transduction cascades, including the ERK and JNK kinase pathways as well as the NF-kappa-B pathway. The encoded protein is activated by autophosphorylation and requires magnesium as a cofactor in phosphorylating other proteins. This protein has E3 ligase activity conferred by a plant homeodomain (PHD) in its N-terminus and phospho-kinase activity conferred by a kinase domain in its C-terminus. [provided by RefSeq, Mar 2012]
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UniProt Comments for MAP3K1

Component of a protein kinase signal transduction cascade (PubMed:9808624). Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4 (PubMed:9808624). May phosphorylate the MAPK8/JNK1 kinase (PubMed:17761173). Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway (PubMed:9808624).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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