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KNTC2 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS855031

MBS855031 is an antibody targeting KNTC2. Also known as KNTC2 Antibody. Reactive in Human, Mouse. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
KNTC2, Antibody
Full Product Name
KNTC2 Antibody
Product Gene Name
Product Synonym Names
HEC1; HEC; Highly expressed in cancer; rich in leucine heptad repeats; KNTC2; NDC80; Retinoblastoma-associated protein HEC; TID3; kinetochore associated 2
Species Reactivity
Human, Mouse
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
KNTC2 antibody detects endogenous levels of total KNTC2 protein.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
73,913 Da
OMIM
607272
3D Structure
ModBase 3D Structure for O14777
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human KNTC2.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-KNTC2 antibody
Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-KNTC2 antibody
WB: 1:500~1:1000
IF: 1:100~1:500
ELISA: 1:20000

Testing Data of anti-KNTC2 antibody

Anti-KNTC2 Antibody – Cat# MBS855031

Western Blot (WB) of anti-KNTC2 antibody

Peptide-+ Western blot analysis of extracts from Jurkat cells, using KNTC2 antibody. Immunofluorescence analysis of HUVEC cells, using KNTC2 antibody.
Anti-KNTC2 Antibody, Western Blot (WB) – Cat# MBS855031

Product References and Citations for anti-KNTC2 antibody

Chen Y., Mol. Cell. Biol. 17:6049-6056(1997). The MGC Project Team, Genome Res. 14:2121-2127(2004). Zheng L., Mol. Cell. Biol. 19:5417-5428(1999).

Other Notes

Small volumes of anti-KNTC2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for KNTC2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
kinetochore protein NDC80 homolog
NCBI Official Synonym Full Names
NDC80, kinetochore complex component
NCBI Official Symbol
NDC80
NCBI Official Synonym Symbols
HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80
UniProt Protein Name
Kinetochore protein NDC80 homolog
UniProt Synonym Protein Names
Highly expressed in cancer protein; Kinetochore protein Hec1; HsHec1; Kinetochore-associated protein 2; Retinoblastoma-associated protein HEC
UniProt Gene Name
NDC80
UniProt Synonym Gene Names
HEC; HEC1; KNTC2; HsHec1
NCBI Protein Information
kinetochore protein NDC80 homolog

NCBI Summary for KNTC2

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]
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UniProt Comments for KNTC2

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:9315664, PubMed:12351790, PubMed:14654001, PubMed:14699129, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:16732327). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:15548592). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020). Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules (PubMed:25743205, PubMed:23891108).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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