Western blot analysis of extracts from HeLa cells and A549 cells, all treated with etoposide (25uM, 24hours), using SPTA2 (Cleaved-Asp1185) antibody.

SPTA2 Antibody

Name: SPTA2 Antibody; Human-Reactive; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS855549
Price: 380 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS855549

MBS855549 is an antibody targeting SPTA2. Also known as SPTA2 (Cleaved-Asp1185) Antibody. Reactive in Human. More details

$380 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

SPTA2, Antibody

Full Product Name

SPTA2 (Cleaved-Asp1185) Antibody

Product Gene Name

anti-SPTA2 antibody

Product Synonym Names

alpha-II spectrin; fodrin alpha; SPCN; spectrin alpha II; spectrin alpha, brain; spectrin, non-erythroid alpha; SPNA2; SPTA2

Species Reactivity

Human

Form/Format

In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Purity/Purification

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity

SPTA2 (Cleaved-Asp1185) antibody detects endogenous levels of fragment of activated SPTA2 resulting from cleavage adjacent to Asp1185.

Preparation and Storage

Store at-20 degree C for 1 year.

Molecular Weight

282,282 Da

OMIM

182810

3D Structure

ModBase 3D Structure for Q13813

Immunogen

The antiserum was produced against synthesized peptide derived from human SPTA2.

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-SPTA2 antibody

Western Blot (WB), ELISA (EIA)

Application Notes for anti-SPTA2 antibody

WB: 1:500-1:1000
ELISA: 1:10000

Product References and Citations for anti-SPTA2 antibody

Moon R.T., J. Biol. Chem. 265:4427-4433(1990). Cianci C.D., Biochemistry 38:15721-15730(1999). Humphray S.J., Nature 429:369-374(2004).

Other Notes

Small volumes of anti-SPTA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SPTA2. It may not necessarily be applicable to this product.

NCBI GI #

194595509

NCBI GeneID

6709

NCBI Accession #

NP_001123910.1

UniProt Primary Accession #

Q13813

UniProt Secondary Accession #

Q13186; Q15324; Q16606; Q59EF1; Q5VXV5; Q5VXV6; Q7Z6M5; Q9P0V0

UniProt Related Accession #

Q13813

GenBank Nucleotide #

NM_001130438.2

NCBI Official Full Name

spectrin alpha chain, non-erythrocytic 1 isoform 1

NCBI Official Synonym Full Names

spectrin alpha, non-erythrocytic 1

NCBI Official Symbol

SPTAN1

NCBI Official Synonym Symbols

NEAS; EIEE5; SPTA2

UniProt Protein Name

Spectrin alpha chain, non-erythrocytic 1

UniProt Synonym Protein Names

Alpha-II spectrin; Fodrin alpha chain; Spectrin, non-erythroid alpha subunit

UniProt Gene Name

SPTAN1

UniProt Synonym Gene Names

NEAS; SPTA2

NCBI Protein Information

spectrin alpha chain, non-erythrocytic 1

NCBI Summary for SPTA2

Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. This gene encodes an alpha spectrin that is specifically expressed in nonerythrocytic cells. The encoded protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in this gene are the cause of early infantile epileptic encephalopathy-5. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]

UniProt Comments for SPTA2

Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.

Research Articles on SPTA2

1. Spectrin may be engaged in regulation of distinct events necessary for the establishment and maturity of the immunological synapse.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SPTA2 antibody

Reference Product	PubMed Publications
SPTBN1 antibody	>1 publications with SPTA2 and SPTBN1

Pathways associated with anti-SPTA2 antibody

Products by Pathway	Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptotic Cleavage Of Cellular Proteins Pathway antibodies	Apoptotic Cleavage Of Cellular Proteins Pathway Diagram
Apoptotic Execution Phase Pathway antibodies	Apoptotic Execution Phase Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Caspase Cascade In Apoptosis Pathway antibodies	Caspase Cascade In Apoptosis Pathway Diagram
Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway antibodies	Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway Diagram
Cell-Cell Communication Pathway antibodies	Cell-Cell Communication Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram

Diseases associated with anti-SPTA2 antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>8 publications with SPTA2 and Nervous System Diseases
Brain Diseases Antibodies	>7 publications with SPTA2 and Brain Diseases
Neoplasms Antibodies	>4 publications with SPTA2 and Neoplasms
Atrophy Antibodies	>3 publications with SPTA2 and Atrophy
Movement Disorders Antibodies	>2 publications with SPTA2 and Movement Disorders
EPILEPTIC ENCEPHALOPATHY, EARLY INFANTILE, 5 Antibodies	>2 publications with SPTA2 and EPILEPTIC ENCEPHALOPATHY, EARLY INFANTILE, 5
Precancerous Conditions Antibodies	>1 publications with SPTA2 and Precancerous Conditions

Organs/Tissues associated with anti-SPTA2 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>4 publications with SPTA2 and Brain
Vascular Antibodies	>3 publications with SPTA2 and Vascular
Nerve Antibodies	>3 publications with SPTA2 and Nerve
Blood Antibodies	>3 publications with SPTA2 and Blood
Bladder Antibodies	>1 publications with SPTA2 and Bladder
Stomach Antibodies	>1 publications with SPTA2 and Stomach
Muscle Antibodies	>1 publications with SPTA2 and Muscle