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MDFI Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS855605

MBS855605 is an antibody targeting MDFI. Also known as MDFI Antibody. Reactive in Human, Mouse. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MDFI, Antibody
Full Product Name
MDFI Antibody
Product Gene Name
Product Synonym Names
I-MF; MyoD family inhibitor; myogenic repressor I-mf
Species Reactivity
Human, Mouse
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
MDFI antibody detects endogenous levels of total MDFI protein.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
25,029 Da
OMIM
604971
3D Structure
ModBase 3D Structure for Q99750
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human MDFI.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-MDFI antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-MDFI antibody
WB: 1:500-1:1000
ELISA: 1:10000

Western Blot (WB) of anti-MDFI antibody

Western blot analysis of extracts from Jurkat cells, using MDFI antibody.
Anti-MDFI Antibody – Cat# MBS855605

Product References and Citations for anti-MDFI antibody

Kraut N., Mamm. Genome 8:618-619(1997). Mungall A.J., Nature 425:805-811(2003). The MGC Project Team, Genome Res. 14:2121-2127(2004).

Other Notes

Small volumes of anti-MDFI antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MDFI. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
myoD family inhibitor isoform 1
NCBI Official Synonym Full Names
MyoD family inhibitor
NCBI Official Symbol
MDFI
NCBI Official Synonym Symbols
I-MF; I-mfa
UniProt Protein Name
MyoD family inhibitor
UniProt Synonym Protein Names
Myogenic repressor I-mf
Protein Family
UniProt Gene Name
MDFI
NCBI Protein Information
myoD family inhibitor

NCBI Summary for MDFI

This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. [provided by RefSeq, Jul 2008]
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UniProt Comments for MDFI

Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation. Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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