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MBD3 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS856044

MBS856044 is an antibody targeting MBD3. Also known as MBD3 Antibody. Reactive in Human, Mouse. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MBD3, Antibody
Full Product Name
MBD3 Antibody
Product Gene Name
Product Synonym Names
Methyl-CpG-binding domain protein 3; Methyl-CpG-binding protein MBD3; MBD3
Species Reactivity
Human, Mouse
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
MBD3 antibody detects endogenous levels of total MBD3 protein.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
29,014 Da
OMIM
603573
3D Structure
ModBase 3D Structure for O95983
Immunogen
The antiserum was produced against synthesized peptide derived from C-terminal of human MBD3.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-MBD3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-MBD3 antibody
WB: 1:500~1:1000
ELISA: 1:5000

Western Blot (WB) of anti-MBD3 antibody

Western blot analysis of extracts from Jurkat cells, using MBD3 antibody.
Anti-MBD3 Antibody – Cat# MBS856044

Product References and Citations for anti-MBD3 antibody

Hendrich B., Mol. Cell. Biol. 18:6538-6547(1998). Grimwood J., Nature 428:529-535(2004). Tatematsu K., Genes Cells 5:677-688(2000).

Other Notes

Small volumes of anti-MBD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MBD3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
methyl-CpG-binding domain protein 3 isoform 1
NCBI Official Synonym Full Names
methyl-CpG binding domain protein 3
NCBI Official Symbol
MBD3
UniProt Protein Name
Methyl-CpG-binding domain protein 3
UniProt Synonym Protein Names
Methyl-CpG-binding protein MBD3
UniProt Gene Name
MBD3
NCBI Protein Information
methyl-CpG-binding domain protein 3

NCBI Summary for MBD3

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. This gene belongs to a family of nuclear proteins which are characterized by the presence of a methyl-CpG binding domain (MBD). The encoded protein is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. Unlike the other family members, the encoded protein is not capable of binding to methylated DNA. The protein mediates the association of metastasis-associated protein 2 with the core histone deacetylase complex. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
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UniProt Comments for MBD3

Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (PubMed:12124384). Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed:24307175). Recruits histone deacetylases and DNA methyltransferases.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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