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TSC2 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS856283

MBS856283 is an antibody targeting TSC2. Also known as TSC2 (Phospho-Tyr1571) Antibody. Reactive in Human, Mouse, Rat. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TSC2, Antibody
Full Product Name
TSC2 (Phospho-Tyr1571) Antibody
Product Gene Name
Product Synonym Names
TSC2; tuberous sclerosis 2 gene; Tuberous sclerosis 2 homolog protein; Tuberous sclerosis 2 protein
Species Reactivity
Human, Mouse, Rat
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificity
TSC2 (Phospho-Tyr1571) antibody detects endogenous levels of TSC2 only when phosphorylated at tyrosine 1571.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
25,773 Da
OMIM
191092
3D Structure
ModBase 3D Structure for P49815
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human TSC2 around the phosphorylation site of tyrosine 1571 (Y-R-YP-T-E).
All Sites
Human: Tyr1571
Mouse: Tyr1572
Rat: Tyr1573
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-TSC2 antibody
Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-TSC2 antibody
IHC: 1:50-1:100
ELISA: 1:5000

Testing Data of anti-TSC2 antibody

P-peptide-+
Anti-TSC2 Antibody – Cat# MBS856283

Immunohistochemistry (IHC) of anti-TSC2 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue using TSC2 (Phospho-Tyr1571) antibody. TSC2 (Phospho-Tyr1571) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.
Anti-TSC2 Antibody, Immunohistochemistry (IHC) – Cat# MBS856283

Product References and Citations for anti-TSC2 antibody

Nellist M., Cell 75:1305-1315(1993). Sampson J.R.; Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases. Xu L., Genomics 27:475-480(1995).

Other Notes

Small volumes of anti-TSC2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TSC2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tuberin isoform 1
NCBI Official Synonym Full Names
TSC complex subunit 2
NCBI Official Symbol
TSC2
NCBI Official Synonym Symbols
LAM; TSC4; PPP1R160
UniProt Protein Name
Tuberin
UniProt Synonym Protein Names
Tuberous sclerosis 2 protein
Protein Family
UniProt Gene Name
TSC2
UniProt Synonym Gene Names
TSC4
NCBI Protein Information
tuberin

NCBI Summary for TSC2

Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
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UniProt Comments for TSC2

In complex with TSC1, this tumor suppressor inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (PubMed:12271141, PubMed:28215400). Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1 (PubMed:15340059). May also play a role in microtubule-mediated protein transport (). Also stimulates the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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