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Histone H2AX (H2AFX) Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS856449

MBS856449 is an antibody targeting H2AFX. Also known as Histone H2AX Antibody. Reactive in Human. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Histone H2AX (H2AFX), Antibody
Full Product Name
Histone H2AX Antibody
Product Gene Name
Product Synonym Names
H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X
Species Reactivity
Human
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
Histone H2AX antibody detects endogenous levels of total Histone H2AX protein.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
15,145 Da
OMIM
601772
3D Structure
ModBase 3D Structure for P16104
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human Histone H2AX.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-H2AFX antibody
Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-H2AFX antibody
WB: 1:500~1:1000
IF: 1:100~1:500
ELISA: 1:20000

Testing Data of anti-H2AFX antibody

Anti-H2AFX Antibody – Cat# MBS856449

Western Blot (WB) of anti-H2AFX antibody

Peptide-+ Western blot analysis of extracts from HT-29 cells, using Histone H2AX antibody. Immunofluorescence analysis of COS7 cells, using Histone H2AX antibody.
Anti-H2AFX Antibody, Western Blot (WB) – Cat# MBS856449

Product References and Citations for anti-H2AFX antibody

The MGC Project Team; Genome Res. 14:2121-2127(2004). Rogakou E.P., J. Biol. Chem. 273:5858-5868(1998). Rogakou E.P., J. Biol. Chem. 275:9390-9395(2000).

Other Notes

Small volumes of anti-H2AFX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for H2AFX. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
histone H2AX
NCBI Official Synonym Full Names
H2A histone family member X
NCBI Official Symbol
H2AFX
NCBI Official Synonym Symbols
H2AX; H2A.X; H2A/X
UniProt Protein Name
Histone H2AX
UniProt Synonym Protein Names
Histone H2A.X
Protein Family
UniProt Gene Name
H2AFX
UniProt Synonym Gene Names
H2AX; H2a/x
NCBI Protein Information
histone H2AX

NCBI Summary for H2AFX

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015]
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UniProt Comments for H2AFX

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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