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WTAP Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS856555

MBS856555 is an antibody targeting WTAP. Also known as WTAP Antibody. Reactive in Human, Mouse. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
WTAP, Antibody
Full Product Name
WTAP Antibody
Product Gene Name
Product Synonym Names
KIAA0105; Putative pre-mRNA splicing regulator female-lethal (2D) homolog; WT1-associated protein; Wilms' tumor 1-associating protein
Species Reactivity
Human, Mouse
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
WTAP antibody detects endogenous levels of total WTAPv protein.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
17,801 Da
OMIM
605442
3D Structure
ModBase 3D Structure for Q15007
Immunogen
The antiserum was produced against synthesized peptide derived from C-terminal of human WTAP.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-WTAP antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-WTAP antibody
WB: 1:500-1:1000
ELISA: 1:1000

Western Blot (WB) of anti-WTAP antibody

Western blot analysis of extracts from HUVEC cells, using WTAP antibody.
Anti-WTAP Antibody – Cat# MBS856555

Product References and Citations for anti-WTAP antibody

Ortega A., J. Biol. Chem. 278:3040-3047(2003). Nagase T., DNA Res. 2:37-43(1995). Mungall A.J., Nature 425:805-811(2003).

Other Notes

Small volumes of anti-WTAP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for WTAP. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
pre-mRNA-splicing regulator WTAP isoform 1
NCBI Official Synonym Full Names
WT1 associated protein
NCBI Official Symbol
WTAP
NCBI Official Synonym Symbols
Mum2
UniProt Protein Name
Pre-mRNA-splicing regulator WTAP
UniProt Synonym Protein Names
Female-lethal(2)D homolog; hFL(2)D; WT1-associated protein; Wilms tumor 1-associating protein
UniProt Gene Name
WTAP
UniProt Synonym Gene Names
KIAA0105; hFL(2)D
NCBI Protein Information
pre-mRNA-splicing regulator WTAP

NCBI Summary for WTAP

The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms. [provided by RefSeq, Jul 2012]
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UniProt Comments for WTAP

Regulatory subunit of the WMM N6-methyltransferase complex, a multiprotein complex that mediates N6-methyladenosine (m6A) methylation of some adenosine residues of some mRNAs and plays a role in the efficiency of mRNA splicing, processing and mRNA stability. Required for accumulation of METTL3 and METTL14 to nuclear speckle (PubMed:24316715, PubMed:24407421, PubMed:24981863). Acts as a mRNA splicing regulator (PubMed:12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (PubMed:17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (PubMed:17095724).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-WTAP antibody

 Reference Product  PubMed Publications
 WT1 antibody  >28 publications with WTAP and WT1
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