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G3BP-1 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS856852

MBS856852 is an antibody targeting G3BP-1. Also known as G3BP-1 (Phospho-Ser232) Antibody. Reactive in Human. More details

$295 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
G3BP-1, Antibody
Full Product Name
G3BP-1 (Phospho-Ser232) Antibody
Product Gene Name
anti-G3BP-1 antibody
Species Reactivity
Human
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificity
G3BP-1 (phospho-Ser232) antibody detects endogenous levels of G3BP-1 only when phosphorylated at serine 232.
Preparation and Storage
Store at-20 degree C for 1 year
Molecular Weight
13,874 Da
OMIM
608431
3D Structure
ModBase 3D Structure for Q13283
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human G3BP-1 around the phosphorylation site of serine 232 (S-S-SP-P-A).

Product Categories/Family for anti-G3BP-1 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-G3BP-1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-G3BP-1 antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:200

Immunohistochemistry (IHC) of anti-G3BP-1 antibody

P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using G3BP-1 (phospho-Ser232) antibody.
Anti-G3BP-1 Antibody – Cat# MBS856852

Western Blot (WB) of anti-G3BP-1 antibody

Western blot analysis of extracts from 293 cells using G3BP-1 (Ab-232) antibody and G3BP-1 (phospho-Ser232) antibody.
Anti-G3BP-1 Antibody, Western Blot (WB) – Cat# MBS856852

Product References and Citations for anti-G3BP-1 antibody

Tao WA, et al. (2005) Nat Methods. 2(8): 591-598. Zheng H, et al. (2005) Mol Cell Proteomics. 4(6):721-730. Beausoleil SA, et al. (2004) Proc Natl Acad Sci U S A.101 (33): 12130-12135. Tourrihre H, et al. (2003) J Cell Biol.160 (6): 823-831.

Other Notes

Small volumes of anti-G3BP-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for G3BP-1. It may not necessarily be applicable to this product.

NCBI GI #
5031703
NCBI GeneID
10146
NCBI Accession #
NP_005745.1
UniProt Primary Accession #
Q13283
UniProt Secondary Accession #
Q5HYE9
UniProt Related Accession #
Q13283
GenBank Nucleotide #
NM_005754.2
NCBI Official Full Name
ras GTPase-activating protein-binding protein 1
NCBI Official Synonym Full Names
G3BP stress granule assembly factor 1
NCBI Official Symbol
G3BP1
NCBI Official Synonym Symbols
G3BP; HDH-VIII
UniProt Protein Name
Ras GTPase-activating protein-binding protein 1
UniProt Synonym Protein Names
ATP-dependent DNA helicase VIII; hDH VIII; GAP SH3 domain-binding protein 1
Protein Family
Ras GTPase-activating protein-binding protein
UniProt Gene Name
G3BP1
UniProt Synonym Gene Names
G3BP; G3BP-1; hDH VIII
NCBI Protein Information
ras GTPase-activating protein-binding protein 1

NCBI Summary for G3BP-1

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]
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UniProt Comments for G3BP-1

May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.
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Research Articles on G3BP-1

1. Activated glucocorticoid receptor induced phosphorylation of v-AKT Murine Thymoma Viral Oncogene Homologue (AKT) kinase, which in turn phosphorylated and promoted nuclear translocation of G3BP1. The nuclear G3BP1 bound to the G3BP1 consensus sequence located on primary miR-15b~16-2 and miR-23a~27a~24-2 to inhibit their maturation.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-G3BP-1 antibody

 Reference Product  PubMed Publications
 USP10 antibody  >6 publications with G3BP-1 and USP10

Diseases associated with anti-G3BP-1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >10 publications with G3BP-1 and Neoplasms
 Lung Diseases Antibodies  >3 publications with G3BP-1 and Lung Diseases
 Drug Toxicity Antibodies  >1 publications with G3BP-1 and Drug Toxicity
 Neoplasm Metastasis Antibodies  >1 publications with G3BP-1 and Neoplasm Metastasis
 Disease Models, Animal Antibodies  >1 publications with G3BP-1 and Disease Models, Animal
 Vascular Diseases Antibodies  >1 publications with G3BP-1 and Vascular Diseases
 Cardiovascular Diseases Antibodies  >1 publications with G3BP-1 and Cardiovascular Diseases
 Carcinoma, Hepatocellular Antibodies  >1 publications with G3BP-1 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >1 publications with G3BP-1 and Liver Neoplasms

Organs/Tissues associated with anti-G3BP-1 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >3 publications with G3BP-1 and Lung
 Mouth Antibodies  >2 publications with G3BP-1 and Mouth
 Liver Antibodies  >2 publications with G3BP-1 and Liver
 Blood Antibodies  >2 publications with G3BP-1 and Blood
 Lymph Antibodies  >1 publications with G3BP-1 and Lymph
 Thymus Antibodies  >1 publications with G3BP-1 and Thymus
 Lymph Node Antibodies  >1 publications with G3BP-1 and Lymph Node
 Skin Antibodies  >1 publications with G3BP-1 and Skin
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