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p56Dok-2 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS856924

MBS856924 is an antibody targeting p56Dok-2. Also known as p56Dok-2 (Phospho-Tyr299) Antibody. Reactive in Human. More details

$295 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
p56Dok-2, Antibody
Full Product Name
p56Dok-2 (Phospho-Tyr299) Antibody
Product Gene Name
Species Reactivity
Human
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificity
p56Dok-2 (phospho-Tyr299) antibody detects endogenous levels of p56Dok-2 only when phosphorylated at tyrosine 299.
Preparation and Storage
Store at-20 degree C for 1 year
Molecular Weight
45,379 Da
OMIM
604997
3D Structure
ModBase 3D Structure for O60496
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human p56Dok-2 around the phosphorylation site of tyrosine 299 (G-E-YP-A-V).
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-p56Dok-2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-p56Dok-2 antibody
WB: 1:500~1:1000
IHC: 1:50~1:100

Immunohistochemistry (IHC) of anti-p56Dok-2 antibody

P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using p56Dok-2 (phospho-Tyr299) antibody.
Anti-p56Dok-2 Antibody – Cat# MBS856924

Western Blot (WB) of anti-p56Dok-2 antibody

Starvation - - - + Peptide - + - - Western blot analysis of extracts from K562 cells, using p56Dok-2 (Ab-299) antibody and p56Dok-2 (phospho-Tyr299) antibody.
Anti-p56Dok-2 Antibody, Western Blot (WB) – Cat# MBS856924

Product References and Citations for anti-p56Dok-2 antibody

Feng Cong, et, al. (1999) Mol. Cell. Biol; 19: 8314-8325. Serge Lemay, et, al. (2000) Mol. Cell. Biol; 20: 2743-2754. Ute Schaeper, et, al.(2000) J. Cell Biol; 149: 1419. Miyuki Honma, et, al. (2006) Genes Cells; 11: 143-151.

Other Notes

Small volumes of anti-p56Dok-2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for p56Dok-2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
docking protein 2 isoform a
NCBI Official Synonym Full Names
docking protein 2
NCBI Official Symbol
DOK2
NCBI Official Synonym Symbols
p56DOK; p56dok-2
UniProt Protein Name
Docking protein 2
UniProt Synonym Protein Names
Downstream of tyrosine kinase 2; p56(dok-2)
UniProt Gene Name
DOK2
NCBI Protein Information
docking protein 2

NCBI Summary for p56Dok-2

The protein encoded by this gene is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. This encoded protein binds p120 (RasGAP) from CML cells. [provided by RefSeq, Jul 2008]
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UniProt Comments for p56Dok-2

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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