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CRK2 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS857330

MBS857330 is an antibody targeting Crk2. Also known as Crk2 (Phospho-Try221) Antibody. Reactive in Human, Mouse, Rat. More details

$295 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Crk2, Antibody
Full Product Name
Crk2 (Phospho-Try221) Antibody
Product Gene Name
Species Reactivity
Human, Mouse, Rat
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificity
Crk2 (Phospho-Tyr221) antibody detects endogenous levels of Crk2 only when phosphorylated at tyrosine 221.
Preparation and Storage
Store at-20 degree C for 1 year
Molecular Weight
22,847 Da
3D Structure
ModBase 3D Structure for Q64010
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Crk2 around the phosphorylation site of tyrosine 221 (G-P-YP-A-Q).
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-Crk2 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-Crk2 antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:4000

Immunohistochemistry (IHC) of anti-Crk2 antibody

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using Crk2 (Phospho-Try221) Antibody.
Anti-CRK2 Antibody – Cat# MBS857330

Product References and Citations for anti-Crk2 antibody

Ogawa S, et al. (1994) Oncogene 9:1669-1678. Abassi Y.A., et al. (2002) EMBO J. 21:4571-4582. Feller S.M., et al. (1994) EMBO J. 13:2341-2351. Ballif B.A., et al. (2008) J. Proteome Res. 7:311-318. Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-1625. Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681.

Other Notes

Small volumes of anti-Crk2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Crk2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
adapter molecule crk isoform 2
NCBI Official Synonym Full Names
v-crk avian sarcoma virus CT10 oncogene homolog
NCBI Official Symbol
Crk
NCBI Official Synonym Symbols
p38; Crk3; Crko; Crk-I; c-Crk; Crk-II; CrkIII; Crk-III
UniProt Protein Name
Adapter molecule crk
UniProt Synonym Protein Names
Proto-oncogene c-Crk; p38
Protein Family
UniProt Gene Name
Crk
UniProt Synonym Gene Names
Crko
NCBI Protein Information
adapter molecule crk

NCBI Summary for Crk2

This gene is part of a family of adapter proteins that mediate formation of signal transduction complexes in response to extracellular stimuli, such as growth and differentiation factors. Protein-protein interactions occur through the SH2 domain, which binds phosphorylated tyrosine residues, and the SH3 domain, which binds proline-rich peptide motifs. These interactions promote recruitment and activation of effector proteins to regulate cell migration, adhesion, and proliferation. In mouse this protein is essential for embryonic development. Alternatively spliced transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq, Mar 2013]
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UniProt Comments for Crk2

Isoform Crk-II: Regulates cell adhesion, spreading and migration. Mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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