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WEE1 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS858225

MBS858225 is an antibody targeting WEE1. Also known as WEE1 (Phospho-Ser642) Antibody. Reactive in Human, Mouse, Rat. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
WEE1, Antibody
Full Product Name
WEE1 (Phospho-Ser642) Antibody
Product Gene Name
Product Synonym Names
EC 2.7.10.2; kinase WEE1; Wee1-like protein kinase; WEE1A; WEE1hu
Species Reactivity
Human, Mouse, Rat
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificity
WEE1 (Phospho-Ser642) antibody detects endogenous levels of WEE1 only when phosphorylated at serine 642.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
49,072 Da
OMIM
193525
3D Structure
ModBase 3D Structure for P30291
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human WEE1 around the phosphorylation site of serine 642 (S-V-SP-L-T).
All Sites
Human: Ser642
Mouse: Ser642
Rat: Ser642
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-WEE1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-WEE1 antibody
WB: 1:500~1:1000
ELISA: 1:5000

Western Blot (WB) of anti-WEE1 antibody

Western blot analysis of extracts from 293 cells, treated with etoposide (25uM, 60mins), using WEE1 (Phospho-Ser642) antibody.
Anti-WEE1 Antibody – Cat# MBS858225

Product References and Citations for anti-WEE1 antibody

Watanabe N., EMBO J. 14:1878-1891(1995). Cichutek A., Cytogenet. Cell Genet. 93:277-283(2001). Igarashi M., Nature 353:80-83(1991).

Other Notes

Small volumes of anti-WEE1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for WEE1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
wee1-like protein kinase isoform 2
NCBI Official Synonym Full Names
WEE1 G2 checkpoint kinase
NCBI Official Symbol
WEE1
NCBI Official Synonym Symbols
WEE1A; WEE1hu
UniProt Protein Name
Wee1-like protein kinase
UniProt Synonym Protein Names
Wee1A kinase
UniProt Gene Name
WEE1
UniProt Synonym Gene Names
WEE1hu
NCBI Protein Information
wee1-like protein kinase

NCBI Summary for WEE1

This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008]
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UniProt Comments for WEE1

Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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