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RPAB1 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS858273

MBS858273 is an antibody targeting RPAB1. Also known as RPAB1 Antibody. Reactive in Human, Mouse, Rat. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RPAB1, Antibody
Full Product Name
RPAB1 Antibody
Product Gene Name
Product Synonym Names
DNA-directed RNA polymerases I; II; and III subunit RPABC1; RNA polymerases I; II; and III subunit ABC1; DNA-directed RNA polymerase II subunit E; RPB5; DNA-directed RNA polymerase II 23 kDa polypeptide; XAP4; POLR2E
Species Reactivity
Human, Mouse, Rat
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
RPAB1 antibody detects endogenous levels of total RPAB1 protein.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
24,551 Da
OMIM
180664
3D Structure
ModBase 3D Structure for P19388
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human RPAB1.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-RPAB1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-RPAB1 antibody
WB: 1:500~1:1000
ELISA: 1:40000

Western Blot (WB) of anti-RPAB1 antibody

Western blot analysis of extracts from MCF-7 cells, using RPAB1 antibody.
Anti-RPAB1 Antibody – Cat# MBS858273

Product References and Citations for anti-RPAB1 antibody

Pati U.K., J. Biol. Chem. 264:13114-13121(1989). Pati U.K., J. Biol. Chem. 266:13468-13468(1991). Cheong J.H., EMBO J. 14:143-150(1995).

Other Notes

Small volumes of anti-RPAB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RPAB1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA-directed RNA polymerases I, II, and III subunit RPABC1 isoform a
NCBI Official Synonym Full Names
RNA polymerase II subunit E
NCBI Official Symbol
POLR2E
NCBI Official Synonym Symbols
RPB5; XAP4; RPABC1; hRPB25; hsRPB5
UniProt Protein Name
DNA-directed RNA polymerases I, II, and III subunit RPABC1
UniProt Synonym Protein Names
DNA-directed RNA polymerase II 23 kDa polypeptide; DNA-directed RNA polymerase II subunit E; RPB5 homolog; XAP4
UniProt Gene Name
POLR2E
UniProt Synonym Gene Names
RNA polymerases I, II, and III subunit ABC1
NCBI Protein Information
DNA-directed RNA polymerases I, II, and III subunit RPABC1

NCBI Summary for RPAB1

This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]
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UniProt Comments for RPAB1

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RPAB1 antibody

 Reference Product  PubMed Publications
 URI1 antibody  >1 publications with RPAB1 and URI1

Organs/Tissues associated with anti-RPAB1 antibody

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