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SMAD3 Antibody – Rabbit

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS858478

MBS858478 is an antibody targeting Smad3. Also known as Smad3 (Phospho-Thr179) Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Smad3, Antibody
Full Product Name
Smad3 (Phospho-Thr179) Antibody
Product Gene Name
anti-Smad3 antibody
Product Synonym Names
JV15-2; MAD-3; MADH3; Mad3; Mothers against DPP homolog 3; Mothers against decapentaplegic homolog 3; SMAD 3; Smad 3
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificity
Smad3 (Phospho-Thr179) antibody detects endogenous levels of Smad3 only when phosphorylated at threonine 179.
Preparation and Storage
Store at-20 degree C for 1 year.
OMIM
114500
3D Structure
ModBase 3D Structure for P84022
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Smad3 around the phosphorylation site of threonine 179 (P-E-TP-P-P).
All Sites
Human: Thr179
Mouse: Thr179
Rat: Thr179

Product Categories/Family for anti-Smad3 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-Smad3 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-Smad3 antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:1000

Immunohistochemistry (IHC) of anti-Smad3 antibody

Immunohistochemistry analysis of paraffin-embedded human thymus gland tissue using Smad3 (Phospho-Thr179) antibody.
P-peptide: - / +
Rabbit Anti-SMAD3 Antibody – Cat# MBS858478

Western Blot (WB) of anti-Smad3 antibody

Western blot analysis of extracts from HeLa cells, treated with TNF (20ng/ml, 2mins), using Smad3 (Phospho-Thr179) antibody.
Rabbit Anti-SMAD3 Antibody, Western Blot (WB) – Cat# MBS858478

Product References and Citations for anti-Smad3 antibody

Zhang Y., Nature 383:168-172(1996). Riggins G.J., Nat. Genet. 13:347-349(1996). Arai T., Cancer Lett. 122:157-163(1998).

Other Notes

Small volumes of anti-Smad3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Smad3. It may not necessarily be applicable to this product.

NCBI GI #
223029440
NCBI GeneID
4088
NCBI Accession #
NP_001138574.1
UniProt Primary Accession #
P84022
UniProt Secondary Accession #
O09064; O09144; O14510; O35273; Q92940; Q93002; A8K4B6; B7Z4Z5; B7Z6M9; B7Z9Q2; F5H383
UniProt Related Accession #
P84022
GenBank Nucleotide #
NM_001145102.1
NCBI Official Full Name
mothers against decapentaplegic homolog 3 isoform 2
NCBI Official Synonym Full Names
SMAD family member 3
NCBI Official Symbol
SMAD3
NCBI Official Synonym Symbols
LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
UniProt Protein Name
Mothers against decapentaplegic homolog 3
UniProt Synonym Protein Names
JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
Protein Family
Mothers against decapentaplegic
UniProt Gene Name
SMAD3
UniProt Synonym Gene Names
MADH3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD 3; Smad3; hSMAD3
NCBI Protein Information
mothers against decapentaplegic homolog 3

NCBI Summary for Smad3

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]
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UniProt Comments for Smad3

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
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Research Articles on Smad3

1. ERK1/2 mediates Heme oxygenase-1 or CO-induced Smad3 phosphorylation at Thr179.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Smad3 antibody

 Reference Product  PubMed Publications
 SMAD2 antibody  >1384 publications with Smad3 and SMAD2
 SMAD4 antibody  >729 publications with Smad3 and SMAD4
 JUN antibody  >407 publications with Smad3 and JUN
 SMAD1 antibody  >169 publications with Smad3 and SMAD1
 MYC antibody  >88 publications with Smad3 and MYC
 CTNNB1 antibody  >85 publications with Smad3 and CTNNB1
 SP1 antibody  >72 publications with Smad3 and SP1
 SKI antibody  >63 publications with Smad3 and SKI
 MAPK1 antibody  >44 publications with Smad3 and MAPK1
 RUNX2 antibody  >37 publications with Smad3 and RUNX2

Pathways associated with anti-Smad3 antibody

 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram

Diseases associated with anti-Smad3 antibody

 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >674 publications with Smad3 and Fibrosis
 Neoplasms Antibodies  >581 publications with Smad3 and Neoplasms
 Disease Models, Animal Antibodies  >245 publications with Smad3 and Disease Models, Animal
 Inflammation Antibodies  >244 publications with Smad3 and Inflammation
 Liver Diseases Antibodies  >175 publications with Smad3 and Liver Diseases
 Kidney Diseases Antibodies  >152 publications with Smad3 and Kidney Diseases
 Cardiovascular Diseases Antibodies  >145 publications with Smad3 and Cardiovascular Diseases
 Breast Neoplasms Antibodies  >100 publications with Smad3 and Breast Neoplasms
 Necrosis Antibodies  >92 publications with Smad3 and Necrosis
 Liver Cirrhosis Antibodies  >91 publications with Smad3 and Liver Cirrhosis

Organs/Tissues associated with anti-Smad3 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >452 publications with Smad3 and Muscle
 Lung Antibodies  >276 publications with Smad3 and Lung
 Skin Antibodies  >195 publications with Smad3 and Skin
 Heart Antibodies  >143 publications with Smad3 and Heart
 Embryonic Tissue Antibodies  >137 publications with Smad3 and Embryonic Tissue
 Intestine Antibodies  >56 publications with Smad3 and Intestine
 Thyroid Antibodies  >28 publications with Smad3 and Thyroid
 Ascites Antibodies  >1 publications with Smad3 and Ascites
 Uncharacterized Tissue Antibodies  >1 publications with Smad3 and Uncharacterized Tissue
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