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eIF2alpha Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS859110

MBS859110 is an antibody targeting eIF2alpha. Also known as eIF2alpha (Phospho-Ser51) Antibody. Reactive in Human, Mouse, Rat. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
eIF2alpha, Antibody
Full Product Name
eIF2alpha (Phospho-Ser51) Antibody
Product Gene Name
Product Synonym Names
EIF2S1; Eukaryotic translation initiation factor 2 subunit 1
Species Reactivity
Human, Mouse, Rat
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificity
eIF2alpha (phospho-Ser51) antibody detects endogenous levels of eIF2alpha only when phosphorylated at serine 51.
Preparation and Storage
Store at-20 degree C for 1 year
Molecular Weight
36,112 Da
OMIM
603907
3D Structure
ModBase 3D Structure for P05198
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human eIF2alpha around the phosphorylation site of serine 51 (E-L-SP-R-R).
All Sites
Human: Ser51
Mouse: Ser52
Rat: Ser51
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-eIF2alpha antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-eIF2alpha antibody
WB: 1:500-1:1000
ELISA: 1:10000

Western Blot (WB) of anti-eIF2alpha antibody

Western blot analysis of extracts from K562 cells untreated or treated with IFN-alpha (1000U/ml, 18 hours), using eIF2alpha (Ab-51) antibody (Line 1 and 2) and eIF2alpha (phospho-Ser51) antibody (Line 3 and 4).
Anti-eIF2alpha Antibody – Cat# MBS859110

Product References and Citations for anti-eIF2alpha antibody

Xavier Saelens, et, al. (2001) J. Biol. Chem; 276: 41620-41628. Hiroyuki Kubota, et, al.(2003) J. Biol. Chem; 278: 20457-20460. Shijian Chu, et, al. (2006) Am J Physiol Lung Cell Mol Physiol; 291: L983-L992. Eileen Connolly, et, al.(2006) Mol. Cell. Biol; 26: 3955-3965.

Other Notes

Small volumes of anti-eIF2alpha antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for eIF2alpha. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
eukaryotic translation initiation factor 2 subunit 1
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 2 subunit alpha
NCBI Official Symbol
EIF2S1
NCBI Official Synonym Symbols
EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
UniProt Protein Name
Eukaryotic translation initiation factor 2 subunit 1
UniProt Synonym Protein Names
Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha
UniProt Gene Name
EIF2S1
UniProt Synonym Gene Names
EIF2A; eIF-2-alpha; eIF-2A; eIF-2alpha
NCBI Protein Information
eukaryotic translation initiation factor 2 subunit 1

NCBI Summary for eIF2alpha

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
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UniProt Comments for eIF2alpha

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-eIF2alpha antibody

Organs/Tissues associated with anti-eIF2alpha antibody

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