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ATP-Citrate Lyase (ACL) Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS859519

MBS859519 is an antibody targeting ACL. Also known as ATP-Citrate Lyase (Ab-454) Antibody. Reactive in Human, Mouse, Rat. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ATP-Citrate Lyase (ACL), Antibody
Full Product Name
ATP-Citrate Lyase (Ab-454) Antibody
Product Gene Name
Product Synonym Names
ATP-citrate (pro-S-)-lyase; ATP-citrate lyase; Citrate cleavage enzyme; EC 2.3.3.8
Species Reactivity
Human, Mouse, Rat
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
ATP-Citrate Lyase (Ab-454) antibody detects endogenous levels of total ATP-Citrate Lyase protein.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
91,099 Da
OMIM
108728
3D Structure
ModBase 3D Structure for P53396
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human ATP-Citrate Lyase around the phosphorylation site of serine 454 (T-A-SP-F-S).
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-ACL antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-ACL antibody
WB: 1:500~1:1000
ELISA: 1:5000

Western Blot (WB) of anti-ACL antibody

Western blot analysis of extracts from COS7 cells, treated with Calyculin (50nM, 30mins), using ATP-Citrate Lyase (Ab-454) antibody.
Anti-ACL Antibody – Cat# MBS859519

Product References and Citations for anti-ACL antibody

Elshourbagy N.A., Eur. J. Biochem. 204:491-499(1992). Lord K.A., Protein Expr. Purif. 9:133-141(1997). Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).

Other Notes

Small volumes of anti-ACL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ACL. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ATP-citrate synthase isoform 1
NCBI Official Synonym Full Names
ATP citrate lyase
NCBI Official Symbol
ACLY
NCBI Official Synonym Symbols
ACL; ATPCL; CLATP
UniProt Protein Name
ATP-citrate synthase
UniProt Synonym Protein Names
ATP-citrate (pro-S-)-lyase; ACL; Citrate cleavage enzyme
UniProt Gene Name
ACLY
UniProt Synonym Gene Names
ACL
NCBI Protein Information
ATP-citrate synthase

NCBI Summary for ACL

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014]
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UniProt Comments for ACL

ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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