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MARK1/2/3/4 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS859757

MBS859757 is an antibody targeting MARK1/2/3/4. Also known as MARK1/2/3/4 (Phospho-Thr215) Antibody. Reactive in Human, Mouse, Rat. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MARK1/2/3/4, Antibody
Full Product Name
MARK1/2/3/4 (Phospho-Thr215) Antibody
Product Gene Name
Product Synonym Names
MAP/microtubule affinity-regulating kinase 1; MARK
Species Reactivity
Human, Mouse, Rat
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificity
MARK1/2/3/4 (Phospho-Thr215) antibody detects endogenous levels of MARK1/2/3/4 only when phosphorylated at threonine 215.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
84,911 Da
OMIM
606511
3D Structure
ModBase 3D Structure for Q9P0L2
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human MARK1/2/3/4 around the phosphorylation site of threonine 215 (L-D-TP-F-C).
All Sites
Human: Thr215/208/235/215
Mouse: Thr215/208/212/215
Rat: Thr215/208/212/-
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-MARK1/2/3/4 antibody
Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-MARK1/2/3/4 antibody
IHC: 1:50-1:100
ELISA: 1:5000

Immunohistochemistry (IHC) of anti-MARK1/2/3/4 antibody

P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MARK1/2/3/4 (Phospho-Thr215) antibody.
Anti-MARK1/2/3/4 Antibody – Cat# MBS859757

Product References and Citations for anti-MARK1/2/3/4 antibody

Lizcano J.M., EMBO J. 23:833-843(2004). Zhou H.J., Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases. Nagase T., DNA Res. 7:143-150(2000).

Other Notes

Small volumes of anti-MARK1/2/3/4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MARK1/2/3/4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
serine/threonine-protein kinase MARK1 isoform 4
NCBI Official Synonym Full Names
microtubule affinity regulating kinase 1
NCBI Official Symbol
MARK1
NCBI Official Synonym Symbols
MARK; Par1c; Par-1c
UniProt Protein Name
Serine/threonine-protein kinase MARK1
UniProt Synonym Protein Names
MAP/microtubule affinity-regulating kinase 1; PAR1 homolog c; Par-1c; Par1c
UniProt Gene Name
MARK1
UniProt Synonym Gene Names
Par-1c; Par1c
NCBI Protein Information
serine/threonine-protein kinase MARK1

UniProt Comments for MARK1/2/3/4

Serine/threonine-protein kinase involved in cell polarity and microtubule dynamics regulation. Phosphorylates DCX, MAP2, MAP4 and MAPT/TAU. Involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-MARK1/2/3/4 antibody

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