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Rhodopsin (RHO) Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS859870

MBS859870 is an antibody targeting RHO. Also known as Rhodopsin (Phospho-Ser334) Antibody. Reactive in Human, Mouse, Rat. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Rhodopsin, Antibody
Full Product Name
Rhodopsin (Phospho-Ser334) Antibody
Product Gene Name
Product Synonym Names
OPN2; OPSD; opsin 2
Species Reactivity
Human, Mouse, Rat
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificity
Rhodopsin (Phospho-Ser334) antibody detects endogenous levels of Rhodopsin only when phosphorylated at serine 334.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
38,893 Da
OMIM
180380
3D Structure
ModBase 3D Structure for P08100
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Rhodopsin around the phosphorylation site of serine 334 (D-A-SP-A-T).
All Sites
Human: Ser334
Mouse: Ser334
Rat: Ser334
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-RHO antibody
Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-RHO antibody
IHC: 1:50-1:100
ELISA: 1:1000

Immunohistochemistry (IHC) of anti-RHO antibody

P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human brain tissue using Rhodopsin (Phospho-Ser334) antibody.
Anti-RHO Antibody – Cat# MBS859870

Product References and Citations for anti-RHO antibody

Edwin S. Lee, Invest. Ophthalmol. Vis. Sci., Jun 2007; 48: 2868-2876. DJ Roof, Invest. Ophthalmol. Vis. Sci., Nov 1994; 35: 4049. Angelika Schmitt, J. Exp. Biol., Apr 2005; 208: 1247-1256. Paul A. Sieving, J. Neurosci., Aug 2001; 21: 5449.

Other Notes

Small volumes of anti-RHO antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RHO. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
rhodopsin
NCBI Official Synonym Full Names
rhodopsin
NCBI Official Symbol
RHO
NCBI Official Synonym Symbols
RP4; OPN2; CSNBAD1
UniProt Protein Name
Rhodopsin
UniProt Synonym Protein Names
Opsin-2
Protein Family
UniProt Gene Name
RHO
UniProt Synonym Gene Names
OPN2
NCBI Protein Information
rhodopsin

NCBI Summary for RHO

The protein encoded by this gene is found in rod cells in the back of the eye and is essential for vision in low-light conditions. The encoded protein binds to 11-cis retinal and is activated when light hits the retinal molecule. Defects in this gene are a cause of congenital stationary night blindness. [provided by RefSeq, Aug 2017]
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UniProt Comments for RHO

Photoreceptor required for image-forming vision at low light intensity (PubMed:8107847, PubMed:7846071). Required for photoreceptor cell viability after birth (PubMed:2215617, PubMed:12566452). Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins (PubMed:8107847, PubMed:28524165, PubMed:26200343, PubMed:28753425). Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling (PubMed:28524165, PubMed:26200343).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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