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NIPA Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS859875

MBS859875 is an antibody targeting NIPA. Also known as NIPA (Phospho-Ser354) Antibody. Reactive in Human, Mouse, Rat. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NIPA, Antibody
Full Product Name
NIPA (Phospho-Ser354) Antibody
Product Gene Name
Product Synonym Names
Nuclear interacting partner of ALK; Nuclear-interacting partner of ALK; ZC3HC1
Species Reactivity
Human, Mouse, Rat
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificity
NIPA (Phospho-Ser354) antibody detects endogenous levels of NIPA only when phosphorylated at serine 354.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
47,771 Da
3D Structure
ModBase 3D Structure for Q86WB0
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human NIPA around the phosphorylation site of serine 354 (T-R-SP-W-D).
All Sites
Human: Ser354
Mouse: Ser353
Rat: Ser353
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-NIPA antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-NIPA antibody
WB: 1:500~1:1000
ELISA: 1:40000

Western Blot (WB) of anti-NIPA antibody

Western blot analysis of extracts from COS7 cells, treated with HU (2nM, 24hours), using NIPA (Phospho-Ser354) antibody.
Anti-NIPA Antibody – Cat# MBS859875

Product References and Citations for anti-NIPA antibody

Ouyang T., J. Biol. Chem. 278:30028-30036(2003). Zhang Q.-H., Genome Res. 10:1546-1560(2000). Ota T., Nat. Genet. 36:40-45(2004).

Other Notes

Small volumes of anti-NIPA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NIPA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
nuclear-interacting partner of ALK isoform 2
NCBI Official Synonym Full Names
zinc finger C3HC-type containing 1
NCBI Official Symbol
ZC3HC1
NCBI Official Synonym Symbols
NIPA; HSPC216
UniProt Protein Name
Nuclear-interacting partner of ALK
UniProt Synonym Protein Names
Nuclear-interacting partner of anaplastic lymphoma kinase; hNIPA; Zinc finger C3HC-type protein 1
Protein Family
UniProt Gene Name
ZC3HC1
UniProt Synonym Gene Names
NIPA; hNIPA
NCBI Protein Information
nuclear-interacting partner of ALK

NCBI Summary for NIPA

This gene encodes an F-box-containing protein that is a component of an SCF-type E3 ubiquitin ligase complex that regulates the onset of cell division. The G2/M transition in the cell cycle requires the interaction of the proteins cyclin B1 and cyclin-dependent kinase 1. The activated ubiquitin ligase complex targets the protein cyclin B1 for degradation, preventing this transition to mitosis. [provided by RefSeq, Aug 2013]
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UniProt Comments for NIPA

Essential component of a SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1 (CCNB1). Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting CCNB1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of CCNB1 in interphase and premature mitotic entry. May have an antiapoptotic role in NPM-ALK-mediated signaling events.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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