Semaphorin 7a, GPI Membrane Anchor (John Milton Hagen Blood Group) (SEMA7A) ELISA Kit for Human

Name: Human SEMA7A ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS900720
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS900720

Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SEMA7A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SEMA7A present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group) (SEMA7A), ELISA Kit

Full Product Name

Human Semaphorin-7A, SEMA7A ELISA Kit

Product Gene Name

SEMA7A elisa kit

Product Synonym Names

Human Semaphorin-7A (SEMA7A) ELISA kit; CD108; CDw108; H-SEMA-K1; H-Sema-L; JMH; MGC126692; MGC126696; SEMAK1; SEMAL; John Milton Hagen blood group H-Sema K1; sema domain; immunoglobulin domain (Ig); and GPI membrane anchor; (semaphorin) 7; semaphorin 7A; GPI membrane anchor (John Milton Hagen blood group)

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates, cell lysates.

Detection Range

0.312 ng/ml -20 ng/ml.

Sensitivity

0.078 ng/ml.

Specificity

This assay has high sensitivity and excellent specificity for detection of human SEMA7A. No significant cross-reactivity or interference between human SEMA7A and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

74,824 Da

OMIM

gene+614745

3D Structure

ModBase 3D Structure for O75326

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of SEMA7A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SEMA7A purchase

MBS900720 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group) (SEMA7A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SEMA7A. The ELISA analytical biochemical technique of the MBS900720 kit is based on SEMA7A antibody-SEMA7A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SEMA7A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SEMA7A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for SEMA7A. It may not necessarily be applicable to this product.

NCBI GI #

225637520

NCBI GeneID

8482

NCBI Accession #

NP_001139501.1

UniProt Primary Accession #

O75326

UniProt Secondary Accession #

Q1XE81; Q1XE82; Q1XE83; Q1XE84; Q3MIY5; B4DDP7; F5H1S0

UniProt Related Accession #

O75326

GenBank Nucleotide #

NM_001146029.1

NCBI Official Full Name

semaphorin-7A isoform 2 preproprotein

NCBI Official Synonym Full Names

semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group)

NCBI Official Symbol

SEMA7A

NCBI Official Synonym Symbols

JMH; CD108; SEMAL; CDw108; SEMAK1; H-Sema-L; H-SEMA-K1

UniProt Protein Name

Semaphorin-7A

UniProt Synonym Protein Names

CDw108; JMH blood group antigen; John-Milton-Hargen human blood group Ag; Semaphorin-K1; Sema K1; Semaphorin-L; Sema L; CD_antigen: CD108

Protein Family

Semaphorin

UniProt Gene Name

SEMA7A

UniProt Synonym Gene Names

CD108; SEMAL; Sema K1; Sema L

UniProt Entry Name

SEM7A_HUMAN

NCBI Protein Information

semaphorin-7A; sema L; sema K1; semaphorin L; semaphorin-L; semaphorin K1; semaphorin-K1; JMH blood group antigen; John Milton Hagen blood group H-Sema K1; john-Milton-Hargen human blood group Ag; sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, 7A; sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (JMH blood group)

NCBI Summary for SEMA7A

The protein encoded by this gene binds to cell surfaces through a glycosylphosphatidylinositol (GPI) linkage. The encoded glycoprotein is found on activated lymphocytes and erythrocytes. This protein may be involved in immunomodulatory and neuronal processes. Defects in this gene can result in loss of bone mineral density (BMD). Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]

UniProt Comments for SEMA7A

SEMA7A: Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes. Belongs to the semaphorin family.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 15q22.3-q23

Cellular Component: membrane; plasma membrane; external side of plasma membrane

Molecular Function: integrin binding; protein binding; receptor activity

Biological Process: integrin-mediated signaling pathway; osteoblast differentiation; axon guidance; axon extension; positive regulation of axon extension; regulation of inflammatory response; olfactory lobe development; immune response; positive regulation of protein amino acid phosphorylation; inflammatory response

Disease: Blood Group, John Milton Hagen System

Research Articles on SEMA7A

1. SEMA7A was expressed in the liver and was increased in the course of liver fibrosis, both in mice and in humans.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with SEMA7A elisa kit

Products by Pathway	Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Other Semaphorin Interactions Pathway antibodies	Other Semaphorin Interactions Pathway Diagram
Semaphorin Interactions Pathway antibodies	Semaphorin Interactions Pathway Diagram

Diseases associated with SEMA7A elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>9 publications with SEMA7A and Inflammation
Lung Diseases Antibodies	>4 publications with SEMA7A and Lung Diseases
Nervous System Diseases Antibodies	>4 publications with SEMA7A and Nervous System Diseases
Brain Diseases Antibodies	>3 publications with SEMA7A and Brain Diseases
Lung Injury Antibodies	>2 publications with SEMA7A and Lung Injury
Cardiovascular Diseases Antibodies	>2 publications with SEMA7A and Cardiovascular Diseases
Skin Neoplasms Antibodies	>1 publications with SEMA7A and Skin Neoplasms
Drug-Induced Liver Injury Antibodies	>1 publications with SEMA7A and Drug-Induced Liver Injury
Weight Loss Antibodies	>1 publications with SEMA7A and Weight Loss
Necrosis Antibodies	>1 publications with SEMA7A and Necrosis

Organs/Tissues associated with SEMA7A elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>17 publications with SEMA7A and Blood
Brain Antibodies	>12 publications with SEMA7A and Brain
Embryonic Tissue Antibodies	>7 publications with SEMA7A and Embryonic Tissue
Skin Antibodies	>6 publications with SEMA7A and Skin
Spleen Antibodies	>4 publications with SEMA7A and Spleen
Bone Antibodies	>4 publications with SEMA7A and Bone
Thymus Antibodies	>3 publications with SEMA7A and Thymus
Testis Antibodies	>3 publications with SEMA7A and Testis
Placenta Antibodies	>3 publications with SEMA7A and Placenta
Lung Antibodies	>2 publications with SEMA7A and Lung