Secreted Frizzled-Related Protein 2 (SFRP2) ELISA Kit for Human

Name: Human SFRP2 ELISA Kit; Secreted Frizzled-Related Protein 2; MyBioSource
Brand: MyBioSource
SKU: MBS902287
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS902287

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SFRP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SFRP2 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

secreted frizzled-related protein 2 (SFRP2), ELISA Kit

Full Product Name

Human Secreted frizzled-related protein 2, SFRP2 ELISA Kit

Product Gene Name

SFRP2 elisa kit

Product Synonym Names

Human Secreted frizzled-related protein 2 (SFRP2) ELISA kit; FRP-2; SARP1; SDF-5; secreted apoptosis related protein 1; secreted frizzled-related protein 2

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, cell culture supernates, tissue homogenates, cell lysates.

Detection Range

0.156 ng/ml -10 ng/ml.

Sensitivity

The minimum detectable dose of human SFRP2 is typically less than 0.039 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Specificity

This assay has high sensitivity and excellent specificity for detection of human SFRP2. No significant cross-reactivity or interference between human SFRP2 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

33,490 Da

OMIM

604157

3D Structure

ModBase 3D Structure for Q96HF1

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of SFRP2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SFRP2 purchase

MBS902287 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the secreted frizzled-related protein 2 (SFRP2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SFRP2. The ELISA analytical biochemical technique of the MBS902287 kit is based on SFRP2 antibody-SFRP2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SFRP2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SFRP2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for SFRP2. It may not necessarily be applicable to this product.

NCBI GI #

48475052

NCBI GeneID

6423

NCBI Accession #

NP_003004.1

UniProt Primary Accession #

Q96HF1

UniProt Secondary Accession #

O14778; Q9HAP5; B3KQR2

UniProt Related Accession #

Q96HF1

GenBank Nucleotide #

NM_003013.2

NCBI Official Full Name

secreted frizzled-related protein 2

NCBI Official Synonym Full Names

secreted frizzled-related protein 2

NCBI Official Symbol

SFRP2

NCBI Official Synonym Symbols

FRP-2; SARP1; SDF-5

UniProt Protein Name

Secreted frizzled-related protein 2

UniProt Synonym Protein Names

Secreted apoptosis-related protein 1; SARP-1

Protein Family

Secreted frizzled-related protein

UniProt Gene Name

SFRP2

UniProt Synonym Gene Names

FRP2; SARP1; FKSG12; UNQ361/PRO697; FRP-2; sFRP-2; SARP-1

UniProt Entry Name

SFRP2_HUMAN

NCBI Protein Information

secreted frizzled-related protein 2; SARP-1; sFRP-2; secreted apoptosis related protein 1; secreted apoptosis-related protein 1

NCBI Summary for SFRP2

This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer. [provided by RefSeq, Jul 2008]

UniProt Comments for SFRP2

SFRP2: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis. Belongs to the secreted frizzled-related protein (sFRP) family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q31.3

Cellular Component: extracellular matrix; extracellular space; integral to membrane

Molecular Function: integrin binding; G-protein coupled receptor activity; Wnt-protein binding; Wnt receptor activity; fibronectin binding; receptor agonist activity

Biological Process: convergent extension involved in axis elongation; negative regulation of Wnt receptor signaling pathway; collagen fibril organization; positive regulation of apoptosis; negative regulation of caspase activity; negative regulation of BMP signaling pathway; negative regulation of cell proliferation; negative regulation of mesodermal cell fate specification; cell-cell signaling; positive regulation of cell proliferation; negative regulation of cell migration; response to nutrient; negative regulation of epithelial cell proliferation; response to drug; negative regulation of peptidyl-tyrosine phosphorylation; negative regulation of JNK activity; male gonad development; positive regulation of cell adhesion mediated by integrin; digestive tract morphogenesis; chondrocyte development; positive regulation of cell growth; positive regulation of peptidyl-serine phosphorylation; patterning of blood vessels; positive regulation of osteoblast differentiation; positive regulation of angiogenesis; neural tube closure; cellular response to extracellular stimulus; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; embryonic digit morphogenesis; negative regulation of transcription, DNA-dependent

Research Articles on SFRP2

1. results show that sFRP-2 is a target gene of hypermethylation in esophageal Basaloid squamous cell carcinoma

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SFRP2 elisa kit

Reference Product	PubMed Publications
WNT4 elisa kit	>12 publications with SFRP2 and WNT4
WNT1 elisa kit	>6 publications with SFRP2 and WNT1
PAX2 elisa kit	>2 publications with SFRP2 and PAX2

Pathways associated with SFRP2 elisa kit

Products by Pathway	Pathway Diagram
Wnt Signaling Pathway NetPath antibodies	Wnt Signaling Pathway NetPath Diagram
Wnt Signaling Pathway antibodies	Wnt Signaling Pathway Diagram
Wnt Signaling Pathway antibodies	Wnt Signaling Pathway Diagram

Diseases associated with SFRP2 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>110 publications with SFRP2 and Neoplasms
Intestinal Diseases Antibodies	>27 publications with SFRP2 and Intestinal Diseases
Colorectal Neoplasms Antibodies	>27 publications with SFRP2 and Colorectal Neoplasms
Adenocarcinoma Antibodies	>18 publications with SFRP2 and Adenocarcinoma
Cardiovascular Diseases Antibodies	>12 publications with SFRP2 and Cardiovascular Diseases
Skin Diseases Antibodies	>11 publications with SFRP2 and Skin Diseases
Carcinoma, Squamous Cell Antibodies	>11 publications with SFRP2 and Carcinoma, Squamous Cell
Fibrosis Antibodies	>10 publications with SFRP2 and Fibrosis
Breast Neoplasms Antibodies	>10 publications with SFRP2 and Breast Neoplasms
Disease Models, Animal Antibodies	>7 publications with SFRP2 and Disease Models, Animal

Organs/Tissues associated with SFRP2 elisa kit

Organ/Tissue Name	Pubmed Publications
Eye Antibodies	>46 publications with SFRP2 and Eye
Bone Antibodies	>33 publications with SFRP2 and Bone
Embryonic Tissue Antibodies	>27 publications with SFRP2 and Embryonic Tissue
Muscle Antibodies	>27 publications with SFRP2 and Muscle
Kidney Antibodies	>12 publications with SFRP2 and Kidney
Connective Tissue Antibodies	>12 publications with SFRP2 and Connective Tissue
Brain Antibodies	>12 publications with SFRP2 and Brain
Heart Antibodies	>11 publications with SFRP2 and Heart
Intestine Antibodies	>11 publications with SFRP2 and Intestine
Nerve Antibodies	>9 publications with SFRP2 and Nerve