Insulin-Like 3 (Leydig Cell) (INSL3) ELISA Kit for Human

Name: Human INSL3 ELISA Kit; Insulin-Like 3 (Leydig Cell); MyBioSource
Brand: MyBioSource
SKU: MBS902482
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS902482

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for INSL3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any INSL3 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

insulin-like 3 (Leydig cell) (INSL3), ELISA Kit

Full Product Name

Human insulin-like 3, INSL3 ELISA Kit

Product Gene Name

INSL3 elisa kit

Product Synonym Names

Human insulin-like 3 (INSL3) ELISA Kit; MGC119818; MGC119819; RLF; RLNL; insulin-like 3; leydig insulin -like hormone; leydig insulin -like peptide; relaxin-like factor b; insulin-like 3 (Leydig cell)

Species Reactivity

Human

Assay Type

Sandwich

Specificity

This assay has high sensitivity and excellent specificity for detection of Human INSL3. No significant cross-reactivity or interference between Human INSL3 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.

Molecular Weight

14,502 Da

OMIM

gene 219050

3D Structure

ModBase 3D Structure for P51460

Detection Wavelength

450 nm

Sample Volume

50-100ul

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of INSL3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for INSL3 purchase

MBS902482 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the insulin-like 3 (Leydig cell) (INSL3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing INSL3. The ELISA analytical biochemical technique of the MBS902482 kit is based on INSL3 antibody-INSL3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect INSL3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, INSL3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for INSL3. It may not necessarily be applicable to this product.

NCBI GI #

388240756

NCBI GeneID

3640

NCBI Accession #

NP_001252516.1

UniProt Primary Accession #

P51460

UniProt Secondary Accession #

Q3KPI5; Q3KPI6; Q6YNB5; Q9UEA2; Q9UPH6; B4DZ72; G3XAG0

UniProt Related Accession #

P51460

GenBank Nucleotide #

NM_001265587.1

NCBI Official Full Name

insulin-like 3 isoform 1

NCBI Official Synonym Full Names

insulin-like 3 (Leydig cell)

NCBI Official Symbol

INSL3

NCBI Official Synonym Symbols

RLF; RLNL; ley-I-L

UniProt Protein Name

Insulin-like 3

UniProt Synonym Protein Names

Leydig insulin-like peptide; Ley-I-L; Relaxin-like factorCleaved into the following 2 chains:Insulin-like 3 B chain; Insulin-like 3 A chain

Protein Family

Insulin

UniProt Gene Name

INSL3

UniProt Synonym Gene Names

RLF; RLNL; Ley-I-L

UniProt Entry Name

INSL3_HUMAN

NCBI Protein Information

insulin-like 3; prepro-INSL3; relaxin-like factor b; leydig insulin-like peptide; leydig insulin -like hormone

NCBI Summary for INSL3

This gene encodes a member of the insulin-like hormone superfamily. The encoded protein is mainly produced in gonadal tissues. Studies of the mouse counterpart suggest that this gene may be involved in the development of urogenital tract and female fertility. This protein may also act as a hormone to regulate growth and differentiation of gubernaculum, and thus mediating intra-abdominal testicular descent. Mutations in this gene may lead to cryptorchidism. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2012]

UniProt Comments for INSL3

INSL3: Seems to play a role in testicular function. May be a trophic hormone with a role in testicular descent in fetal life. Is a ligand for LGR8 receptor. Defects in INSL3 seems to be a cause of cryptorchidism (CRYPTO); also known as impaired testicular descent. It is one of the most frequent congenital abnormalities in humans, involving 2-5% of male births. Cryptorchidism is associated with increased risk of infertility and testicular cancer. The frequency of INSL3 gene mutations as a cause of cryptorchidism is low. Belongs to the insulin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19p13.2-p12

Cellular Component: extracellular space; perinuclear region of cytoplasm; extracellular region

Molecular Function: G-protein-coupled receptor binding; protease binding; hormone activity; insulin receptor binding; receptor binding

Biological Process: negative regulation of cell proliferation; cell-cell signaling; in utero embryonic development; male gonad development; positive regulation of cell proliferation; positive regulation of cAMP biosynthetic process; spermatogenesis; G-protein signaling, adenylate cyclase inhibiting pathway; oocyte maturation; negative regulation of apoptosis

Disease: Cryptorchidism, Unilateral Or Bilateral

Research Articles on INSL3

1. The aim of this study was to assess plasma INSL3 in patients with osteoporosis and Klinefelter's Syndrome compared to healthy males.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with INSL3 elisa kit

Reference Product	PubMed Publications
RXFP2 elisa kit	>70 publications with INSL3 and RXFP2

Pathways associated with INSL3 elisa kit

Products by Pathway	Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
G Alpha (s) Signalling Events Pathway antibodies	G Alpha (s) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Interleukin Receptor SHC Signaling Pathway antibodies	Interleukin Receptor SHC Signaling Pathway Diagram
Interleukin-2 Signaling Pathway antibodies	Interleukin-2 Signaling Pathway Diagram
Interleukin-3, 5 And GM-CSF Signaling Pathway antibodies	Interleukin-3, 5 And GM-CSF Signaling Pathway Diagram
Interleukin-7 Signaling Pathway antibodies	Interleukin-7 Signaling Pathway Diagram

Diseases associated with INSL3 elisa kit

Disease Name	Pubmed Publications
Cryptorchidism Antibodies	>102 publications with INSL3 and Cryptorchidism
Genital Diseases, Male Antibodies	>89 publications with INSL3 and Genital Diseases, Male
Gonadal Disorders Antibodies	>87 publications with INSL3 and Gonadal Disorders
Urogenital Abnormalities Antibodies	>83 publications with INSL3 and Urogenital Abnormalities
Testicular Diseases Antibodies	>80 publications with INSL3 and Testicular Diseases
Infertility, Male Antibodies	>29 publications with INSL3 and Infertility, Male
Disorders of Sex Development Antibodies	>12 publications with INSL3 and Disorders of Sex Development
Neoplasms Antibodies	>10 publications with INSL3 and Neoplasms
Infertility, Female Antibodies	>10 publications with INSL3 and Infertility, Female
Prenatal Exposure Delayed Effects Antibodies	>10 publications with INSL3 and Prenatal Exposure Delayed Effects

Organs/Tissues associated with INSL3 elisa kit

Organ/Tissue Name	Pubmed Publications
Testis Antibodies	>150 publications with INSL3 and Testis
Blood Antibodies	>40 publications with INSL3 and Blood
Brain Antibodies	>11 publications with INSL3 and Brain
Placenta Antibodies	>4 publications with INSL3 and Placenta