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Inter-Alpha (Globulin) Inhibitor H3 (ITIH3) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS902708

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ITIH3. Standards or samples are added to the appropriate microtiter plate wells with… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
inter-alpha (globulin) inhibitor H3 (ITIH3), ELISA Kit
Full Product Name
Human Inter-alpha-trypsin inhibitor heavy chain H3, ITIH3 ELISA Kit
Product Gene Name
Product Synonym Names
Human Inter-alpha-trypsin inhibitor heavy chain H3 (ITIH3) ELISA kit; H3P; inter-alpha (globulin) inhibitor; H3 polypeptide; inter-alpha-trypsin inhibitor heavy chain H3; pre-alpha (globulin) inhibitor; H3 polypeptide; inter-alpha (globulin) inhibitor H3
Species Reactivity
Human
Assay Type
Competitive
Samples
Serum, plasma, tissue homogenates
Detection Range
78.13 ng/ml-5000 ng/ml
Sensitivity
19.53 ng/ml
Specificity
This assay has high sensitivity and excellent specificity for detection of Human ITIH3. No significant cross-reactivity or interference between Human ITIH3 and analogues was observed.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Molecular Weight
99,849 Da
OMIM
146650
3D Structure
ModBase 3D Structure for Q06033
Detection Wavelength
450 nm
Sample Volume
50-100ul
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of ITIH3 elisa kit

Human ITIH3 ELISA Kit – Cat# MBS902708

Other Notes

Small volumes of ITIH3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ITIH3 purchase

MBS902708 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the inter-alpha (globulin) inhibitor H3 (ITIH3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ITIH3. The ELISA analytical biochemical technique of the MBS902708 kit is based on ITIH3 antibody-ITIH3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ITIH3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ITIH3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Proteases cleave unwanted proteins into small easier to digest peptides. To prevent attacks on essential proteins, these enzymes are inhibited by inter alpha trypsin inhibitor heavy chain H3 ITIH3. To accurately study this interplay, MyBiosource has an ITIH 3 ELISA kit available. This ITIH3 ELISA has excellent dynamic range ( ng ml ) and is shipped complete with antibody bound plate, secondary antibodies, standard curve H3 polypeptide, detection reagent and stop solution. Speed and accuracy are the name of the game for small molecule discovery and we have you covered with this H3 ITIH3 ELISA kit and related kits integrin alpha and alpha globulin inhibitor ELISA kit human. We pride ourselves in providing the best reagents for precision sample results. Whether your needs are molecular biology or intercellular adhesion molecule biology, have confidence your samples will have publishable results. Order today at MyBiosource.com.

Additional Details

NCBI/Uniprot data below describe general gene information for ITIH3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
inter-alpha-trypsin inhibitor heavy chain H3 preproprotein
NCBI Official Synonym Full Names
inter-alpha-trypsin inhibitor heavy chain 3
NCBI Official Symbol
ITIH3
NCBI Official Synonym Symbols
H3P
UniProt Protein Name
Inter-alpha-trypsin inhibitor heavy chain H3
UniProt Synonym Protein Names
Serum-derived hyaluronan-associated protein; SHAP
UniProt Gene Name
ITIH3
UniProt Synonym Gene Names
ITI heavy chain H3; ITI-HC3; Inter-alpha-inhibitor heavy chain 3; SHAP
UniProt Entry Name
ITIH3_HUMAN
NCBI Protein Information
inter-alpha-trypsin inhibitor heavy chain H3; SHAP; ITI-HC3; ITI heavy chain H3; inter-alpha (globulin) inhibitor H3; inter-alpha-inhibitor heavy chain 3; serum-derived hyaluronan-associated protein; pre-alpha (globulin) inhibitor, H3 polypeptide; inter-alpha (globulin) inhibitor, H3 polypeptide

NCBI Summary for ITIH3

The inter-alpha-trypsin inhibitors (ITI) are a family of structurally related plasma serine protease inhibitors involved in extracellular matrix stabilization and in prevention of tumor metastasis. The ITI family contains multiple proteins made up of a light chain (see MIM 176870) and a variable number of heavy chains (Salier et al., 1987 [PubMed 2446322]; Himmelfarb et al., 2004 [PubMed 14744536]).[supplied by OMIM, Nov 2009]
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UniProt Comments for ITIH3

ITIH3: May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes. Belongs to the ITIH family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Inhibitor

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity

Biological Process: hyaluronan metabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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