Stem-Loop Binding (SLBP) ELISA Kit for Human

Name: Human SLBP ELISA Kit; Stem-Loop Binding; MyBioSource
Brand: MyBioSource
SKU: MBS904587
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS904587

Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SLBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLBP present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

stem-loop binding protein (SLBP), ELISA Kit

Full Product Name

Human Histone RNA hairpin-binding protein, SLBP ELISA Kit

Product Gene Name

SLBP elisa kit

Product Synonym Names

Human Histone RNA hairpin-binding protein (SLBP) ELISA kit; HBP; hairpin binding protein; histone; histone binding protein; histone stem-loop binding protein; stem-loop (histone) binding protein; stem-loop binding protein

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates, cell lysates.

Detection Range

15.6 pg/ml -1000 pg/ml.

Sensitivity

3.9 pg/ml.

Specificity

This assay has high sensitivity and excellent specificity for detection of human SLBP. No significant cross-reactivity or interference between human SLBP and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

31,286 Da

OMIM

602422

3D Structure

ModBase 3D Structure for Q14493

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of SLBP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SLBP purchase

MBS904587 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the stem-loop binding protein (SLBP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SLBP. The ELISA analytical biochemical technique of the MBS904587 kit is based on SLBP antibody-SLBP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SLBP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SLBP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for SLBP. It may not necessarily be applicable to this product.

NCBI GI #

5729862

NCBI GeneID

7884

NCBI Accession #

NP_006518.1

UniProt Primary Accession #

Q14493

UniProt Secondary Accession #

B3KRJ5

UniProt Related Accession #

Q14493

GenBank Nucleotide #

NM_006527.2

NCBI Official Full Name

histone RNA hairpin-binding protein

NCBI Official Synonym Full Names

stem-loop binding protein

NCBI Official Symbol

SLBP

NCBI Official Synonym Symbols

HBP

UniProt Protein Name

Histone RNA hairpin-binding protein

UniProt Synonym Protein Names

Histone stem-loop-binding protein

Protein Family

Oocyte-specific histone RNA stem-loop-binding protein

UniProt Gene Name

SLBP

UniProt Synonym Gene Names

HBP

UniProt Entry Name

SLBP_HUMAN

NCBI Protein Information

histone RNA hairpin-binding protein; histone binding protein; hairpin binding protein, histone; histone stem-loop binding protein; histone stem-loop-binding protein; stem-loop (histone) binding protein

NCBI Summary for SLBP

This gene encodes a protein that binds to the stem-loop structure in replication-dependent histone mRNAs. Histone mRNAs do not contain introns or polyadenylation signals, and are processed by endonucleolytic cleavage. The stem-loop structure is essential for efficient processing but this structure also controls the transport, translation and stability of histone mRNAs. Expression of the protein is regulated during the cell cycle, increasing more than 10-fold during the latter part of G1. [provided by RefSeq, Jul 2008]

UniProt Comments for SLBP

SLBP: RNA-binding protein involved in the histone pre-mRNA processing. Binds the stem-loop structure of replication-dependent histone pre-mRNAs and contributes to efficient 3'-end processing by stabilizing the complex between histone pre-mRNA and U7 small nuclear ribonucleoprotein (snRNP), via the histone downstream element (HDE). Plays an important role in targeting mature histone mRNA from the nucleus to the cytoplasm and to the translation machinery. Stabilizes mature histone mRNA and could be involved in cell-cycle regulation of histone gene expression. Involved in the mechanism by which growing oocytes accumulate histone proteins that support early embryogenesis. Binds to the 5' side of the stem-loop structure of histone pre-mRNAs. Monomer. SLBP/pre-mRNA complex interacts with ZNF473. Interacts with the Importin alpha/Importin beta receptor, LSM1, MIF4GD, TNPO3 and UPF1. Interaction with LSM1 occurs when histone mRNA is being rapidly degraded during the S phase. Found in a ternary complex with ERI1 and the stem-loop structure of the 3' end of histone mRNA. Associates with polyribosomes. Identified in a histone pre-mRNA complex, at least composed of ERI1, LSM11, SLBP, SNRPB, SYNCRIP and YBX1. Binds in a cooperative manner with ERI1 to the mature 3'-end of histone mRNAs. Widely expressed. Belongs to the SLBP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: nucleoplasm; cytoplasm; nucleus; ribonucleoprotein complex; cytosol

Molecular Function: mRNA binding; protein binding

Biological Process: transcription from RNA polymerase II promoter; mRNA export from nucleus; mRNA transport; histone mRNA metabolic process; DNA replication during S phase; histone mRNA 3'-end processing; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription

Research Articles on SLBP

1. C-terminal extension of Lsm4 interacts directly with the histone mRNP, contacting both SLBP and 3'hExo.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SLBP elisa kit

Reference Product	PubMed Publications
ZNF473 elisa kit	>3 publications with SLBP and ZNF473
LSM10 elisa kit	>3 publications with SLBP and LSM10

Pathways associated with SLBP elisa kit

Products by Pathway	Pathway Diagram
Cleavage Of Growing Transcript In The Termination Region Pathway antibodies	Cleavage Of Growing Transcript In The Termination Region Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies	Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
Processing Of Capped Intronless Pre-mRNA Pathway antibodies	Processing Of Capped Intronless Pre-mRNA Pathway Diagram
RNA Polymerase II Transcription Pathway antibodies	RNA Polymerase II Transcription Pathway Diagram
RNA Polymerase II Transcription Termination Pathway antibodies	RNA Polymerase II Transcription Termination Pathway Diagram
SLBP Dependent Processing Of Replication-Dependent Histone Pre-mRNAs Pathway antibodies	SLBP Dependent Processing Of Replication-Dependent Histone Pre-mRNAs Pathway Diagram
Transport Of Mature Transcript To Cytoplasm Pathway antibodies	Transport Of Mature Transcript To Cytoplasm Pathway Diagram
Transport Of Mature MRNAs Derived From Intronless Transcripts Pathway antibodies	Transport Of Mature MRNAs Derived From Intronless Transcripts Pathway Diagram
Transport Of The SLBP Dependant Mature MRNA Pathway antibodies	Transport Of The SLBP Dependant Mature MRNA Pathway Diagram

Diseases associated with SLBP elisa kit

Disease Name	Pubmed Publications
Death Antibodies	>2 publications with SLBP and Death
Neoplasms Antibodies	>2 publications with SLBP and Neoplasms
Cardiovascular Diseases Antibodies	>1 publications with SLBP and Cardiovascular Diseases
Immune System Diseases Antibodies	>1 publications with SLBP and Immune System Diseases
Carcinoma Antibodies	>1 publications with SLBP and Carcinoma
Vascular Diseases Antibodies	>1 publications with SLBP and Vascular Diseases
Drug Toxicity Antibodies	>1 publications with SLBP and Drug Toxicity
Infertility, Female Antibodies	>1 publications with SLBP and Infertility, Female
Necrosis Antibodies	>1 publications with SLBP and Necrosis

Organs/Tissues associated with SLBP elisa kit

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>2 publications with SLBP and Muscle
Blood Antibodies	>2 publications with SLBP and Blood
Bone Antibodies	>1 publications with SLBP and Bone
Spleen Antibodies	>1 publications with SLBP and Spleen
Stomach Antibodies	>1 publications with SLBP and Stomach
Liver Antibodies	>1 publications with SLBP and Liver