Vasohibin 1 (VASH1) ELISA Kit for Human

Name: Human VASH1 ELISA Kit; Vasohibin 1; MyBioSource
Brand: MyBioSource
SKU: MBS904711
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS904711

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VASH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VASH1 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

vasohibin 1 (VASH1), ELISA Kit

Full Product Name

Human Vasohibin-1, VASH1 ELISA Kit

Product Gene Name

VASH1 elisa kit

Product Synonym Names

Human Vasohibin-1 (VASH1) ELISA kit; KIAA1036; ; vasohibin 1

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates

Detection Range

62.5 pg/ml - 4000 pg/ml.

Sensitivity

The minimum detectable dose of human VASH1 is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Specificity

This assay has high sensitivity and excellent specificity for detection of human VASH1. No significant cross-reactivity or interference between human VASH1 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

40,957 Da

OMIM

609011

3D Structure

ModBase 3D Structure for Q7L8A9

Inter-assay

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of VASH1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VASH1 purchase

MBS904711 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the vasohibin 1 (VASH1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing VASH1. The ELISA analytical biochemical technique of the MBS904711 kit is based on VASH1 antibody-VASH1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect VASH1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, VASH1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for VASH1. It may not necessarily be applicable to this product.

NCBI GI #

7662454

NCBI GeneID

22846

NCBI Accession #

NP_055724.1

UniProt Primary Accession #

Q7L8A9

UniProt Secondary Accession #

Q96H02; Q9UBF4; Q9Y629

UniProt Related Accession #

Q7L8A9

GenBank Nucleotide #

NM_014909.4

NCBI Official Full Name

vasohibin-1

NCBI Official Synonym Full Names

vasohibin 1

NCBI Official Symbol

VASH1

NCBI Official Synonym Symbols

KIAA1036

UniProt Protein Name

Vasohibin-1

Protein Family

Vasohibin

UniProt Gene Name

VASH1

UniProt Synonym Gene Names

KIAA1036; VASH

UniProt Entry Name

VASH1_HUMAN

NCBI Protein Information

vasohibin-1

UniProt Comments for VASH1

VASH1: Angiogenesis inhibitor. Inhibits migration, proliferation and network formation by endothelial cells as well as angiogenesis. This inhibitory effect is selective to endothelial cells as it does not affect the migration of smooth muscle cells or fibroblasts. Does not affect the proliferation of cancer cells in vitro, but inhibits tumor growth and tumor angiogenesis. Acts in an autocrine manner. Inhibits artery neointimal formation and macrophage infiltration. Exhibits heparin-binding activity. Belongs to the vasohibin family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: extracellular space; endoplasmic reticulum; apical part of cell; cytoplasm

Biological Process: negative regulation of angiogenesis; negative regulation of endothelial cell proliferation; response to wounding; angiogenesis; negative regulation of blood vessel endothelial cell migration; cell cycle arrest

Research Articles on VASH1

1. High Vasohibin-1 expression is associated with colorectal cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with VASH1 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>11 publications with VASH1 and Neoplasms
Carcinoma Antibodies	>7 publications with VASH1 and Carcinoma
Vascular Diseases Antibodies	>6 publications with VASH1 and Vascular Diseases
Cardiovascular Diseases Antibodies	>5 publications with VASH1 and Cardiovascular Diseases
Adenocarcinoma Antibodies	>3 publications with VASH1 and Adenocarcinoma
Neoplasm Metastasis Antibodies	>2 publications with VASH1 and Neoplasm Metastasis
Uterine Neoplasms Antibodies	>2 publications with VASH1 and Uterine Neoplasms
Kidney Diseases Antibodies	>2 publications with VASH1 and Kidney Diseases
Melanoma Antibodies	>2 publications with VASH1 and Melanoma
Endometrial Neoplasms Antibodies	>1 publications with VASH1 and Endometrial Neoplasms

Organs/Tissues associated with VASH1 elisa kit

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>37 publications with VASH1 and Vascular
Blood Antibodies	>16 publications with VASH1 and Blood
Eye Antibodies	>6 publications with VASH1 and Eye
Muscle Antibodies	>5 publications with VASH1 and Muscle
Bone Antibodies	>3 publications with VASH1 and Bone
Kidney Antibodies	>2 publications with VASH1 and Kidney
Embryonic Tissue Antibodies	>2 publications with VASH1 and Embryonic Tissue
Lung Antibodies	>2 publications with VASH1 and Lung
Uterus Antibodies	>1 publications with VASH1 and Uterus
Testis Antibodies	>1 publications with VASH1 and Testis