• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Semaphorin-4d (SEMA4D) ELISA Kit for Rat

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS009066

Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used to determine the level of SEMA4D (hereafter termed "analyte") in… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Semaphorin-4D (SEMA4D), ELISA Kit
Full Product Name
Rat Semaphorin-4D ELISA Kit
Product Gene Name
SEMA4D elisa kit
Species Reactivity
Rat
Assay Type
Quantitative Sandwich
Detection Range
0.25ng/ml-8ng/ml
Sensitivity
0.1ng/ml
Preparation and Storage
Store all reagents at 2-8 degree C
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Molecular Weight
96,150 Da
Chromosome Location
Chromosome: 9; NC_000009.11 (91975706..92094611, complement). Location: 9q22.2
OMIM
601866
3D Structure
ModBase 3D Structure for Q92854

Other Notes

Small volumes of SEMA4D elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SEMA4D purchase

MBS009066 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Semaphorin-4D (SEMA4D) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SEMA4D. The ELISA analytical biochemical technique of the MBS009066 kit is based on SEMA4D antibody-SEMA4D antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SEMA4D antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SEMA4D. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for SEMA4D. It may not necessarily be applicable to this product.

NCBI GI #
214010218
NCBI GeneID
10507
NCBI Accession #
NP_006369.3
UniProt Primary Accession #
Q92854
UniProt Secondary Accession #
Q7Z5S4; Q8N8B0; B2RPM6
UniProt Related Accession #
Q92854
GenBank Nucleotide #
NM_006378.3
NCBI Official Full Name
semaphorin-4D isoform 1
NCBI Official Synonym Full Names
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
NCBI Official Symbol
SEMA4D
NCBI Official Synonym Symbols
CD100; SEMAJ; coll-4; C9orf164; M-sema-G
UniProt Protein Name
Semaphorin-4D
UniProt Synonym Protein Names
A8; BB18; GR3
Protein Family
Semaphorin
UniProt Gene Name
SEMA4D
UniProt Synonym Gene Names
C9orf164; CD100; SEMAJ
UniProt Entry Name
SEM4D_HUMAN
NCBI Protein Information
semaphorin-4D; A8; GR3; BB18; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D

UniProt Comments for SEMA4D

SEMA4D: a single-pass type I membrane protein that my play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q22.2

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; receptor activity; semaphorin receptor binding; receptor binding

Biological Process: negative regulation of peptidyl-tyrosine phosphorylation; axon guidance; regulation of dendrite morphogenesis; negative regulation of cell adhesion; negative regulation of transcription from RNA polymerase II promoter; positive regulation of phosphoinositide 3-kinase cascade; regulation of cell shape; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of osteoblast differentiation; immune response; positive regulation of protein amino acid phosphorylation; cell adhesion; positive regulation of collateral sprouting; regulation of cell projection organization and biogenesis; positive regulation of cell migration; negative regulation of apoptosis
Show more

Research Articles on SEMA4D

1. copy number loss of the Sema4D gene region may play a role in the etiology of acetabular dysplasia

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SEMA4D elisa kit

 Reference Product  PubMed Publications
 PLXNB1 elisa kit  >57 publications with SEMA4D and PLXNB1
 CD72 elisa kit  >31 publications with SEMA4D and CD72
 MET elisa kit  >17 publications with SEMA4D and MET
 ERBB2 elisa kit  >6 publications with SEMA4D and ERBB2
 PTPRC elisa kit  >5 publications with SEMA4D and PTPRC
 RND1 elisa kit  >4 publications with SEMA4D and RND1
 RRAS elisa kit  >3 publications with SEMA4D and RRAS
 ARHGEF12 elisa kit  >2 publications with SEMA4D and ARHGEF12
 ARHGEF11 elisa kit  >2 publications with SEMA4D and ARHGEF11
 GRLF1 elisa kit  >1 publications with SEMA4D and GRLF1

Pathways associated with SEMA4D elisa kit

 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Other Semaphorin Interactions Pathway antibodies  Other Semaphorin Interactions Pathway Diagram
 Sema4D In Semaphorin Signaling Pathway antibodies  Sema4D In Semaphorin Signaling Pathway Diagram
 Sema4D Induced Cell Migration And Growth-cone Collapse Pathway antibodies  Sema4D Induced Cell Migration And Growth-cone Collapse Pathway Diagram
 Sema4D Mediated Inhibition Of Cell Attachment And Migration Pathway antibodies  Sema4D Mediated Inhibition Of Cell Attachment And Migration Pathway Diagram
 Semaphorin Interactions Pathway antibodies  Semaphorin Interactions Pathway Diagram

Diseases associated with SEMA4D elisa kit

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >31 publications with SEMA4D and Neoplasms
 Nervous System Diseases Antibodies  >19 publications with SEMA4D and Nervous System Diseases
 Brain Diseases Antibodies  >9 publications with SEMA4D and Brain Diseases
 Inflammation Antibodies  >8 publications with SEMA4D and Inflammation
 Disease Models, Animal Antibodies  >6 publications with SEMA4D and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >6 publications with SEMA4D and Cardiovascular Diseases
 Kidney Diseases Antibodies  >4 publications with SEMA4D and Kidney Diseases
 Necrosis Antibodies  >3 publications with SEMA4D and Necrosis
 Nerve Degeneration Antibodies  >2 publications with SEMA4D and Nerve Degeneration
 Cerebral Infarction Antibodies  >2 publications with SEMA4D and Cerebral Infarction

Organs/Tissues associated with SEMA4D elisa kit

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >83 publications with SEMA4D and Nerve
 Blood Antibodies  >64 publications with SEMA4D and Blood
 Brain Antibodies  >22 publications with SEMA4D and Brain
 Embryonic Tissue Antibodies  >15 publications with SEMA4D and Embryonic Tissue
 Kidney Antibodies  >7 publications with SEMA4D and Kidney
 Skin Antibodies  >5 publications with SEMA4D and Skin
 Lymph Node Antibodies  >5 publications with SEMA4D and Lymph Node
 Lung Antibodies  >4 publications with SEMA4D and Lung
 Bone Marrow Antibodies  >4 publications with SEMA4D and Bone Marrow
 Spinal Cord Antibodies  >4 publications with SEMA4D and Spinal Cord
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000