Myosin Regulatory Light Chain Interacting (MYLIP) ELISA Kit for Human

Name: Human MYLIP ELISA Kit; Myosin Regulatory Light Chain Interacting; MyBioSource
Brand: MyBioSource
SKU: MBS906926
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS906926

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MYLIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MYLIP present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

myosin regulatory light chain interacting protein (MYLIP), ELISA Kit

Full Product Name

Human E3 ubiquitin-protein ligase MYLIP, MYLIP ELISA Kit

Product Gene Name

MYLIP elisa kit

Product Synonym Names

Human E3 ubiquitin-protein ligase MYLIP (MYLIP) ELISA kit; RP1-13D10.1; IDOL; MIR; band 4.1 superfamily member BZF1; cellular modulator of immune recognition (c-MIR) ; myosin regulatory light chain interacting protein

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates, cell lysates

Detection Range

15.6 pg/ml-1000 pg/ml

Sensitivity

< 3.9 pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of human MYLIP. No significant cross-reactivity or interference between human MYLIP and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

49,910 Da

OMIM

610082

3D Structure

ModBase 3D Structure for Q8WY64

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of MYLIP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MYLIP purchase

MBS906926 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the myosin regulatory light chain interacting protein (MYLIP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MYLIP. The ELISA analytical biochemical technique of the MBS906926 kit is based on MYLIP antibody-MYLIP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MYLIP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MYLIP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for MYLIP. It may not necessarily be applicable to this product.

NCBI GI #

38788243

NCBI GeneID

29116

NCBI Accession #

NP_037394.2

UniProt Primary Accession #

Q8WY64

UniProt Secondary Accession #

Q5TIA4; Q9BU73; Q9NRL9; Q9UHE7

UniProt Related Accession #

Q8WY64

GenBank Nucleotide #

NM_013262.3

NCBI Official Full Name

E3 ubiquitin-protein ligase MYLIP

NCBI Official Synonym Full Names

myosin regulatory light chain interacting protein

NCBI Official Symbol

MYLIP

NCBI Official Synonym Symbols

MIR; IDOL

UniProt Protein Name

E3 ubiquitin-protein ligase MYLIP

UniProt Synonym Protein Names

Inducible degrader of the LDL-receptor; Idol; Myosin regulatory light chain interacting protein; MIR

Protein Family

E3 ubiquitin-protein ligase

UniProt Gene Name

MYLIP

UniProt Synonym Gene Names

BZF1; IDOL; BM-023; PP5242; Idol; MIR

UniProt Entry Name

MYLIP_HUMAN

NCBI Protein Information

E3 ubiquitin-protein ligase MYLIP; band 4.1 superfamily member BZF1; inducible degrader of the LDL-receptor; cellular modulator of immune recognition (c-MIR); myosin regulatory light chain-interacting protein; E3 ubiquitin ligase-inducible degrader of the low density lipoprotein receptor

NCBI Summary for MYLIP

The ERM protein family members ezrin, radixin, and moesin are cytoskeletal effector proteins linking actin to membrane-bound proteins at the cell surface. Myosin regulatory light chain interacting protein (MYLIP) is a novel ERM-like protein that interacts with myosin regulatory light chain and inhibits neurite outgrowth. [provided by RefSeq, Jul 2008]

UniProt Comments for MYLIP

MYLIP: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol- dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; Ubiquitin conjugating system; Motility/polarity/chemotaxis; Ubiquitin ligase; EC 6.3.2.19; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: cytoskeleton; extrinsic to membrane; cytoplasm; plasma membrane; intracellular

Molecular Function: protein binding; zinc ion binding; cytoskeletal protein binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: nervous system development; cholesterol homeostasis; protein destabilization; protein ubiquitination during ubiquitin-dependent protein catabolic process; positive regulation of protein catabolic process; protein ubiquitination; regulation of low-density lipoprotein receptor catabolic process; cell motility

Research Articles on MYLIP

1. evidence for the existence of an LXR-IDOL-mediated internalization pathway for the LDLR that is distinct from that used for lipoprotein uptake

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MYLIP elisa kit

Reference Product	PubMed Publications
CNPY2 elisa kit	>2 publications with MYLIP and CNPY2

Diseases associated with MYLIP elisa kit

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>2 publications with MYLIP and Nervous System Diseases
Neoplasms Antibodies	>2 publications with MYLIP and Neoplasms
Cell Transformation, Neoplastic Antibodies	>1 publications with MYLIP and Cell Transformation, Neoplastic
Carcinoma Antibodies	>1 publications with MYLIP and Carcinoma
Cardiovascular Diseases Antibodies	>1 publications with MYLIP and Cardiovascular Diseases
Brain Diseases Antibodies	>1 publications with MYLIP and Brain Diseases
Neoplasms, Experimental Antibodies	>1 publications with MYLIP and Neoplasms, Experimental
Head and Neck Neoplasms Antibodies	>1 publications with MYLIP and Head and Neck Neoplasms
Liver Diseases Antibodies	>1 publications with MYLIP and Liver Diseases
Carcinoma, Squamous Cell Antibodies	>1 publications with MYLIP and Carcinoma, Squamous Cell

Organs/Tissues associated with MYLIP elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>10 publications with MYLIP and Blood
Heart Antibodies	>5 publications with MYLIP and Heart
Muscle Antibodies	>3 publications with MYLIP and Muscle
Brain Antibodies	>3 publications with MYLIP and Brain
Nerve Antibodies	>2 publications with MYLIP and Nerve
Bone Antibodies	>2 publications with MYLIP and Bone
Thymus Antibodies	>1 publications with MYLIP and Thymus
Bone Marrow Antibodies	>1 publications with MYLIP and Bone Marrow
Embryonic Tissue Antibodies	>1 publications with MYLIP and Embryonic Tissue
Vascular Antibodies	>1 publications with MYLIP and Vascular