Peptidyl Arginine Deiminase, Type IV (PADI4) ELISA Kit for Mouse

Name: Mouse PADI4 ELISA Kit; Peptidyl Arginine Deiminase, Type IV; MyBioSource
Brand: MyBioSource
SKU: MBS906945
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS906945

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PADI4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PADI4 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

peptidyl arginine deiminase, type IV (PADI4), ELISA Kit

Full Product Name

Mouse Protein-arginine deiminase type-4, PADI4 ELISA Kit

Product Gene Name

PADI4 elisa kit

Product Synonym Names

Mouse Protein-arginine deiminase type-4 (PADI4) ELISA kit; PAD; PAD4; PADI5; PDI4; PDI5; HL-60 PAD; PADI-H protein; peptidyl arginine deiminase; type V; protein-arginine deiminase; peptidyl arginine deiminase; type IV

Species Reactivity

Mouse

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates, cell lysates

Detection Range

4.7 pg/ml-300 pg/ml

Sensitivity

< 1.17 pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of mouse PADI4. No significant cross-reactivity or interference between mouse PADI4 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

74,415 Da

3D Structure

ModBase 3D Structure for Q9Z183

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PADI4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PADI4 purchase

MBS906945 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the peptidyl arginine deiminase, type IV (PADI4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PADI4. The ELISA analytical biochemical technique of the MBS906945 kit is based on PADI4 antibody-PADI4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PADI4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PADI4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PADI4. It may not necessarily be applicable to this product.

NCBI GI #

156938252

NCBI GeneID

18602

NCBI Accession #

NP_035191.2

UniProt Primary Accession #

Q9Z183

UniProt Secondary Accession #

Q75WC7; A2AMU3

UniProt Related Accession #

Q9Z183

GenBank Nucleotide #

NM_011061.2

NCBI Official Full Name

protein-arginine deiminase type-4

NCBI Official Synonym Full Names

peptidyl arginine deiminase, type IV

NCBI Official Symbol

Padi4

NCBI Official Synonym Symbols

Pad4; Pdi4

UniProt Protein Name

Protein-arginine deiminase type-4

UniProt Synonym Protein Names

Peptidylarginine deiminase IV; Protein-arginine deiminase type IV

Protein Family

Protein-arginine deiminase

UniProt Gene Name

Padi4

UniProt Synonym Gene Names

Pdi4

UniProt Entry Name

PADI4_MOUSE

NCBI Protein Information

protein-arginine deiminase type-4; PAD type IV; peptidylarginine deiminase IV; protein-arginine deiminase type IV

UniProt Comments for PADI4

PADI4: Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1. Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA). It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. Belongs to the protein arginine deiminase family.

Protein type: Hydrolase; EC 3.5.3.15; Nuclear receptor co-regulator

Cellular Component: cytoplasm; nucleus

Molecular Function: arginine deiminase activity; hydrolase activity; metal ion binding; calcium ion binding; protein-arginine deiminase activity

Biological Process: chromatin remodeling; nucleosome assembly; regulation of transcription, DNA-dependent; transcription, DNA-dependent; immune system process; stem cell maintenance; innate immune response; chromatin modification; peptidyl-citrulline biosynthetic process from peptidyl-arginine

Research Articles on PADI4

1. Selective inhibition of peptidylarginine deiminase IV with Cl-amidine blocked NET formation, reduced atherosclerotic lesion area, and delayed time to carotid artery thrombosis in a photochemical injury model.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with PADI4 elisa kit

Disease Name	Pubmed Publications
Arthritis, Rheumatoid Antibodies	>91 publications with PADI4 and Arthritis, Rheumatoid
Inflammation Antibodies	>8 publications with PADI4 and Inflammation
Neoplasms Antibodies	>8 publications with PADI4 and Neoplasms
Lupus Erythematosus, Systemic Antibodies	>7 publications with PADI4 and Lupus Erythematosus, Systemic
Skin Diseases Antibodies	>4 publications with PADI4 and Skin Diseases
Necrosis Antibodies	>3 publications with PADI4 and Necrosis
Asthma Antibodies	>2 publications with PADI4 and Asthma
Disease Models, Animal Antibodies	>2 publications with PADI4 and Disease Models, Animal
Mental Disorders Antibodies	>1 publications with PADI4 and Mental Disorders

Organs/Tissues associated with PADI4 elisa kit

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>4 publications with PADI4 and Bone
Lung Antibodies	>3 publications with PADI4 and Lung
Bone Marrow Antibodies	>1 publications with PADI4 and Bone Marrow
Thymus Antibodies	>1 publications with PADI4 and Thymus
Skin Antibodies	>1 publications with PADI4 and Skin