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ERBB Receptor Feedback Inhibitor 1 (ERRFI1) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS907410

Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ERRFI1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ERRFI1 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ERBB receptor feedback inhibitor 1 (ERRFI1), ELISA Kit
Full Product Name
Human ERBB receptor feedback inhibitor 1, ERRFI1 ELISA Kit
Product Gene Name
ERRFI1 elisa kit
Product Synonym Names
Human ERBB receptor feedback inhibitor 1 (ERRFI1) ELISA kit; CTA-215D11.2; GENE-33; MIG-6; MIG6; RALT; mitogen-inducible gene 6; mitogen-inducible gene 6 protein; receptor-associated late transducer; ERBB receptor feedback inhibitor 1
Species Reactivity
Human
Assay Type
Sandwich
Samples
Serum, plasma, tissue homogenates, Cell lysates
Detection Range
31.25 pg/ml-2000 pg/ml.
Sensitivity
The minimum detectable dose of human ERRFI1 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Specificity
This assay has high sensitivity and excellent specificity for detection of human ERRFI1. No significant cross-reactivity or interference between human ERRFI1 and analogues was observed.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Molecular Weight
50,560 Da
OMIM
608069
3D Structure
ModBase 3D Structure for Q9UJM3
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of ERRFI1 elisa kit

Human ERRFI1 ELISA Kit – Cat# MBS907410

Other Notes

Small volumes of ERRFI1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ERRFI1 purchase

MBS907410 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ERBB receptor feedback inhibitor 1 (ERRFI1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ERRFI1. The ELISA analytical biochemical technique of the MBS907410 kit is based on ERRFI1 antibody-ERRFI1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ERRFI1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ERRFI1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ERRFI1. It may not necessarily be applicable to this product.

NCBI GI #
10047086
NCBI GeneID
54206
NCBI Accession #
NP_061821.1
UniProt Primary Accession #
Q9UJM3
UniProt Secondary Accession #
Q9NTG9; Q9UD05; B2RDX9
UniProt Related Accession #
Q9UJM3
GenBank Nucleotide #
NM_018948.3
NCBI Official Full Name
ERBB receptor feedback inhibitor 1
NCBI Official Synonym Full Names
ERBB receptor feedback inhibitor 1
NCBI Official Symbol
ERRFI1
NCBI Official Synonym Symbols
MIG6; RALT; MIG-6; GENE-33
UniProt Protein Name
ERBB receptor feedback inhibitor 1
UniProt Synonym Protein Names
Mitogen-inducible gene 6 protein; MIG-6
Protein Family
ERBB receptor feedback inhibitor
UniProt Gene Name
ERRFI1
UniProt Synonym Gene Names
MIG6; MIG-6
UniProt Entry Name
ERRFI_HUMAN
NCBI Protein Information
ERBB receptor feedback inhibitor 1; mitogen-inducible gene 6 protein; receptor-associated late transducer

NCBI Summary for ERRFI1

ERRFI1 is a cytoplasmic protein whose expression is upregulated with cell growth (Wick et al., 1995 [PubMed 7641805]). It shares significant homology with the protein product of rat gene-33, which is induced during cell stress and mediates cell signaling (Makkinje et al., 2000 [PubMed 10749885]; Fiorentino et al., 2000 [PubMed 11003669]).[supplied by OMIM, Mar 2008]
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UniProt Comments for ERRFI1

MIG-6: Negative regulator of EGFR signaling in skin morphogenesis. Acts as a negative regulator for several EGFR family members, including ERBB2, ERBB3 and ERBB4. Inhibits EGFR catalytic activity by interfering with its dimerization. Inhibits autophosphorylation of EGFR, ERBB2 and ERBB4. Important for normal keratinocyte proliferation and differentiation. Plays a role in modulating the response to steroid hormones in the uterus. Required for normal response to progesterone in the uterus and for fertility. Mediates epithelial estrogen responses in the uterus by regulating ESR1 levels and activation. Important for regulation of endometrium cell proliferation. Important for normal prenatal and perinatal lung development. Interacts with ERBB2. Interacts with EGFR. Levels are very low in quiescent cells. Up-regulated by mitogens. Belongs to the MIG6 family.

Protein type: Tumor suppressor; Inhibitor

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: extrinsic to internal side of plasma membrane; cytoplasm; nucleus

Molecular Function: protein binding; protein kinase binding

Biological Process: negative regulation of epidermal growth factor receptor activity; skin morphogenesis; response to stress; negative regulation of protein amino acid autophosphorylation; regulation of keratinocyte differentiation; alveolus development
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Research Articles on ERRFI1

1. SPRY2 and MIG6 are important regulators of wild-type and mutant EGFR trafficking and points to an EGFR expression-independent function of SPRY2 in the regulation of ERK activity that may impact cellular sensitivity to EGFR inhibitors

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ERRFI1 elisa kit

 Reference Product  PubMed Publications
 EGFR elisa kit  >6 publications with ERRFI1 and EGFR
 ERBB2 elisa kit  >1 publications with ERRFI1 and ERBB2

Pathways associated with ERRFI1 elisa kit

 Products by Pathway  Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram

Diseases associated with ERRFI1 elisa kit

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >6 publications with ERRFI1 and Neoplasms
 Skin Neoplasms Antibodies  >2 publications with ERRFI1 and Skin Neoplasms
 Infertility, Female Antibodies  >1 publications with ERRFI1 and Infertility, Female
 Lung Diseases Antibodies  >1 publications with ERRFI1 and Lung Diseases
 Prostatic Neoplasms Antibodies  >1 publications with ERRFI1 and Prostatic Neoplasms
 Neoplasms, Experimental Antibodies  >1 publications with ERRFI1 and Neoplasms, Experimental
 Breast Neoplasms Antibodies  >1 publications with ERRFI1 and Breast Neoplasms
 Hyperplasia Antibodies  >1 publications with ERRFI1 and Hyperplasia

Organs/Tissues associated with ERRFI1 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2 publications with ERRFI1 and Blood
 Skin Antibodies  >2 publications with ERRFI1 and Skin
 Lung Antibodies  >2 publications with ERRFI1 and Lung
 Uterus Antibodies  >2 publications with ERRFI1 and Uterus
 Nerve Antibodies  >1 publications with ERRFI1 and Nerve
 Bone Antibodies  >1 publications with ERRFI1 and Bone
 Prostate Antibodies  >1 publications with ERRFI1 and Prostate
 Brain Antibodies  >1 publications with ERRFI1 and Brain
 Embryonic Tissue Antibodies  >1 publications with ERRFI1 and Embryonic Tissue
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