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Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS907475

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ENPP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP)… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ectonucleotide pyrophosphatase/phosphodiesterase 2 (ENPP2), ELISA Kit
Full Product Name
Mouse Ectonucleotide pyrophosphatase/phosphodiesterase family member 2, ENPP2 ELISA Kit
Product Gene Name
ENPP2 elisa kit
Product Synonym Names
Mouse Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 (ENPP2) ELISA kit; ATX; ATX-X; AUTOTAXIN; FLJ26803; LysoPLD; NPP2; PD-IALPHA; PDNP2; autotaxin; autotaxin-t; phosphodiesterase I/nucleotide pyrophosphatase 2; plasma lysophospholipase D; ectonucleotide pyrophosphatase/phosphodiesterase 2
Species Reactivity
Mouse
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, tissue homogenates
Detection Range
72 ng/ml-2500 ng/ml
Sensitivity
< 36 ng/ml
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse ENPP2. No significant cross-reactivity or interference between mouse ENPP2 and analogues was observed.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess.
Molecular Weight
98,885 Da
3D Structure
ModBase 3D Structure for Q9R1E6
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of ENPP2 elisa kit

Mouse ENPP2 ELISA Kit – Cat# MBS907475

Other Notes

Small volumes of ENPP2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ENPP2 purchase

MBS907475 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ectonucleotide pyrophosphatase/phosphodiesterase 2 (ENPP2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ENPP2. The ELISA analytical biochemical technique of the MBS907475 kit is based on ENPP2 antibody-ENPP2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ENPP2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ENPP2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ENPP2. It may not necessarily be applicable to this product.

NCBI GI #
209862963
NCBI GeneID
18606
NCBI Accession #
NP_001129549.1
UniProt Primary Accession #
Q9R1E6
UniProt Secondary Accession #
Q6PDE0; Q99LG9; A8UH85; A8UH93; B2ZP54
UniProt Related Accession #
Q9R1E6
GenBank Nucleotide #
NM_001136077.2
NCBI Official Full Name
ectonucleotide pyrophosphatase/phosphodiesterase family member 2 isoform 1 preproprotein
NCBI Official Synonym Full Names
ectonucleotide pyrophosphatase/phosphodiesterase 2
NCBI Official Symbol
Enpp2
NCBI Official Synonym Symbols
ATX; Npps2; Pdnp2; lysoPLD; PD-Ialpha
UniProt Protein Name
Ectonucleotide pyrophosphatase/phosphodiesterase family member 2
UniProt Synonym Protein Names
Autotaxin; Extracellular lysophospholipase D; LysoPLD
Protein Family
Ectonucleotide pyrophosphatase/phosphodiesterase family
UniProt Gene Name
Enpp2
UniProt Synonym Gene Names
Npps2; Pdnp2; E-NPP 2; LysoPLD
UniProt Entry Name
ENPP2_MOUSE
NCBI Protein Information
ectonucleotide pyrophosphatase/phosphodiesterase family member 2; E-NPP 2; autotaxin; extracellular lysophospholipase D; phosphodiesterase I/nucleotide pyrophosphatase 2

UniProt Comments for ENPP2

ENPP2: Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility- related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; Lipid Metabolism - ether lipid; EC 3.1.4.39; Phosphodiesterase

Cellular Component: Golgi apparatus; extracellular space; integral to plasma membrane; cytoplasm; extracellular region

Molecular Function: phosphodiesterase I activity; nucleotide diphosphatase activity; nucleic acid binding; hydrolase activity; zinc ion binding; metal ion binding; calcium ion binding; catalytic activity; scavenger receptor activity; alkylglycerophosphoethanolamine phosphodiesterase activity; lysophospholipase activity; polysaccharide binding

Biological Process: positive regulation of focal adhesion formation; phospholipid catabolic process; metabolic process; positive regulation of cell proliferation; immune response; positive regulation of oligodendrocyte differentiation; lipid metabolic process; negative regulation of cell-matrix adhesion; chemotaxis; regulation of angiogenesis; regulation of cell migration; lipid catabolic process
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Research Articles on ENPP2

1. Autotaxin generates lysophosphatidic acid from lysophophatidylcholine. The role of this pathway in T-lymphocyte homing, inflammation, and airway remodeling is studied and inhibitors are tested. Review.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with ENPP2 elisa kit

 Products by Pathway  Pathway Diagram
 Ether Lipid Metabolism Pathway antibodies  Ether Lipid Metabolism Pathway Diagram
 Ether Lipid Metabolism Pathway antibodies  Ether Lipid Metabolism Pathway Diagram

Diseases associated with ENPP2 elisa kit

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >12 publications with ENPP2 and Neoplasms
 Inflammation Antibodies  >6 publications with ENPP2 and Inflammation
 Carcinoma Antibodies  >5 publications with ENPP2 and Carcinoma
 Breast Neoplasms Antibodies  >5 publications with ENPP2 and Breast Neoplasms
 Adenoma Antibodies  >3 publications with ENPP2 and Adenoma
 Neoplasm Metastasis Antibodies  >3 publications with ENPP2 and Neoplasm Metastasis
 Fibrosis Antibodies  >3 publications with ENPP2 and Fibrosis
 Lung Neoplasms Antibodies  >2 publications with ENPP2 and Lung Neoplasms
 Depressive Disorder Antibodies  >2 publications with ENPP2 and Depressive Disorder
 Ovarian Neoplasms Antibodies  >2 publications with ENPP2 and Ovarian Neoplasms

Organs/Tissues associated with ENPP2 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >11 publications with ENPP2 and Brain
 Lung Antibodies  >5 publications with ENPP2 and Lung
 Blood Antibodies  >3 publications with ENPP2 and Blood
 Pituitary Gland Antibodies  >2 publications with ENPP2 and Pituitary Gland
 Prostate Antibodies  >2 publications with ENPP2 and Prostate
 Muscle Antibodies  >2 publications with ENPP2 and Muscle
 Embryonic Tissue Antibodies  >2 publications with ENPP2 and Embryonic Tissue
 Heart Antibodies  >2 publications with ENPP2 and Heart
 Liver Antibodies  >1 publications with ENPP2 and Liver
 Epididymis Antibodies  >1 publications with ENPP2 and Epididymis
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