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Secreted Frizzled-Related Protein 4 (SFRP4) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS907810

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SFRP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SFRP4 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
secreted frizzled-related protein 4 (SFRP4), ELISA Kit
Full Product Name
Mouse Secreted frizzled-related protein 4, SFRP4 ELISA Kit
Product Gene Name
SFRP4 elisa kit
Product Synonym Names
Mouse Secreted frizzled-related protein 4 (SFRP4) ELISA kit; FRP-4; FRPHE; MGC26498; secreted frizzled-related protein 4; secreted frizzled-related protein 4; secreted frizzled-related protein 4; secreted frizzled-related protein 4
Species Reactivity
Mouse
Samples
Serum, plasma, cell culture supernates, tissue homogenates
Detection Range
0.47 ng/ml -30 ng/mL
Sensitivity
<0.11 ng/mL
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse SFRP4. No significant cross-reactivity or interference between mouse SFRP4 and analogues was observed.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Molecular Weight
40,342 Da
3D Structure
ModBase 3D Structure for Q9Z1N6
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of SFRP4 elisa kit

Mouse SFRP4 ELISA Kit – Cat# MBS907810

Other Notes

Small volumes of SFRP4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SFRP4 purchase

MBS907810 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the secreted frizzled-related protein 4 (SFRP4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SFRP4. The ELISA analytical biochemical technique of the MBS907810 kit is based on SFRP4 antibody-SFRP4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SFRP4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SFRP4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for SFRP4. It may not necessarily be applicable to this product.

NCBI GI #
7710094
NCBI GeneID
20379
NCBI Accession #
NP_057896.1
UniProt Primary Accession #
Q9Z1N6
UniProt Secondary Accession #
Q91ZX9
UniProt Related Accession #
Q9Z1N6
GenBank Nucleotide #
NM_016687.3
NCBI Official Full Name
secreted frizzled-related sequence protein 4
NCBI Official Synonym Full Names
secreted frizzled-related protein 4
NCBI Official Symbol
Sfrp4
UniProt Protein Name
Secreted frizzled-related sequence protein 4
Protein Family
Secreted frizzled-related protein
UniProt Gene Name
Sfrp4
UniProt Synonym Gene Names
FRP-4; sFRP-4
UniProt Entry Name
SFRP4_MOUSE
NCBI Protein Information
secreted frizzled-related sequence protein 4; FRP-4; sFRP-4; frizzled-related protein 4

UniProt Comments for SFRP4

SFRP4: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling. Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake. Belongs to the secreted frizzled-related protein (sFRP) family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; cell surface; cytoplasm; extracellular region; nucleus

Molecular Function: Wnt-protein binding

Biological Process: negative regulation of JNK cascade; negative regulation of cell proliferation; Wnt receptor signaling pathway; positive regulation of apoptosis; negative regulation of transcription factor activity; multicellular organismal development; positive regulation of epidermal cell differentiation; positive regulation of receptor internalization; phosphate ion homeostasis; cell differentiation
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Research Articles on SFRP4

1. studies indicate that mutations neither in sFRP1 nor in sFRP4 are a common cause of craniotubular hyperostoses

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with SFRP4 elisa kit

 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Wnt Signaling Pathway antibodies  Wnt Signaling Pathway Diagram
 Wnt Signaling Pathway antibodies  Wnt Signaling Pathway Diagram
 Wnt Signaling Pathway antibodies  Wnt Signaling Pathway Diagram

Diseases associated with SFRP4 elisa kit

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >84 publications with SFRP4 and Neoplasms
 Carcinoma Antibodies  >37 publications with SFRP4 and Carcinoma
 Adenocarcinoma Antibodies  >17 publications with SFRP4 and Adenocarcinoma
 Leukemia Antibodies  >15 publications with SFRP4 and Leukemia
 Uterine Neoplasms Antibodies  >12 publications with SFRP4 and Uterine Neoplasms
 Carcinoma, Squamous Cell Antibodies  >10 publications with SFRP4 and Carcinoma, Squamous Cell
 Breast Neoplasms Antibodies  >9 publications with SFRP4 and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >9 publications with SFRP4 and Cell Transformation, Neoplastic
 Colorectal Neoplasms Antibodies  >9 publications with SFRP4 and Colorectal Neoplasms
 Endometrial Neoplasms Antibodies  >8 publications with SFRP4 and Endometrial Neoplasms

Organs/Tissues associated with SFRP4 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >28 publications with SFRP4 and Bone
 Eye Antibodies  >18 publications with SFRP4 and Eye
 Uterus Antibodies  >10 publications with SFRP4 and Uterus
 Connective Tissue Antibodies  >9 publications with SFRP4 and Connective Tissue
 Joint Antibodies  >6 publications with SFRP4 and Joint
 Embryonic Tissue Antibodies  >6 publications with SFRP4 and Embryonic Tissue
 Bladder Antibodies  >5 publications with SFRP4 and Bladder
 Brain Antibodies  >5 publications with SFRP4 and Brain
 Skin Antibodies  >4 publications with SFRP4 and Skin
 Pituitary Gland Antibodies  >2 publications with SFRP4 and Pituitary Gland
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