Glutathione Peroxidase 4 (Phospholipid Hydroperoxidase) (GPX4) ELISA Kit for Human

Name: Human GPX4 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS908073
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS908073

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GPX4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GPX4 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

glutathione peroxidase 4 (phospholipid hydroperoxidase) (GPX4), ELISA Kit

Full Product Name

Human Phospholipid hydroperoxide glutathione peroxidase, mitochondrial, GPX4 ELISA Kit

Product Gene Name

GPX4 elisa kit

Product Synonym Names

Human Phospholipid hydroperoxide glutathione peroxidase; mitochondrial (GPX4) ELISA kit; MCSP; PHGPx; snGPx; snPHGPx; glutathione peroxidase 4; phospholipid hydroperoxidase; phospholipid hydroperoxide glutathione peroxidase; mitochondrial; sperm nucleus glutathione peroxidase; glutathione peroxidase 4 (phospholipid hydroperoxidase)

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates

Detection Range

7.8 uU/ml-500 uU/ml.

Sensitivity

The minimum detectable dose of human GPX4 is typically less than 1.95 uU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Specificity

This assay has high sensitivity and excellent specificity for detection of human GPX4. No significant cross-reactivity or interference between human GPX4 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

22,175 Da

OMIM

138322

3D Structure

ModBase 3D Structure for P36969

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of GPX4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GPX4 purchase

MBS908073 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the glutathione peroxidase 4 (phospholipid hydroperoxidase) (GPX4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GPX4. The ELISA analytical biochemical technique of the MBS908073 kit is based on GPX4 antibody-GPX4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GPX4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GPX4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GPX4. It may not necessarily be applicable to this product.

NCBI GI #

90903238

NCBI GeneID

2879

NCBI Accession #

NP_001034936.1

UniProt Primary Accession #

P36969

UniProt Secondary Accession #

O43381; Q6PJ59; Q9UPK2

UniProt Related Accession #

P36969

GenBank Nucleotide #

NM_001039847.2

NCBI Official Full Name

phospholipid hydroperoxide glutathione peroxidase, mitochondrial isoform B

NCBI Official Synonym Full Names

glutathione peroxidase 4

NCBI Official Symbol

GPX4

NCBI Official Synonym Symbols

MCSP; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx

UniProt Protein Name

Phospholipid hydroperoxide glutathione peroxidase, mitochondrial

UniProt Synonym Protein Names

Glutathione peroxidase 4; GPx-4; GSHPx-4

Protein Family

Phospholipid hydroperoxide glutathione peroxidase

UniProt Gene Name

GPX4

UniProt Synonym Gene Names

PHGPx; GPx-4; GSHPx-4

UniProt Entry Name

GPX4_HUMAN

NCBI Protein Information

phospholipid hydroperoxide glutathione peroxidase, mitochondrial; phospholipid hydroperoxidase; sperm nucleus glutathione peroxidase

NCBI Summary for GPX4

This gene encodes a member of the glutathione peroxidase protein family. Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. The encoded protein has been identified as a moonlighting protein based on its ability to serve dual functions as a peroxidase as well as a structural protein in mature spermatozoa. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

UniProt Comments for GPX4

GPX4: Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. Belongs to the glutathione peroxidase family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Nuclear envelope; Oxidoreductase; Mitochondrial; Lipid Metabolism - arachidonic acid; Other Amino Acids Metabolism - glutathione; EC 1.11.1.12

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: mitochondrion; nucleus; cytosol

Molecular Function: selenium binding; glutathione binding; phospholipid-hydroperoxide glutathione peroxidase activity; glutathione peroxidase activity

Biological Process: glutathione metabolic process; lipoxygenase pathway; hydrogen peroxide catabolic process; phospholipid metabolic process; multicellular organismal development; regulation of inflammatory response; arachidonic acid metabolic process; response to oxidative stress; response to estradiol stimulus; aging

Research Articles on GPX4

1. A membrane glycoprotein GPX4 was shown to play a significant role in gamete interactions.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with GPX4 elisa kit

Products by Pathway	Pathway Diagram
Folate Metabolism Pathway antibodies	Folate Metabolism Pathway Diagram
Glutathione Metabolism Pathway antibodies	Glutathione Metabolism Pathway Diagram
Glutathione Metabolism Pathway antibodies	Glutathione Metabolism Pathway Diagram
Glutathione Metabolism Pathway antibodies	Glutathione Metabolism Pathway Diagram
Selenium Metabolism And Selenoproteins Pathway antibodies	Selenium Metabolism And Selenoproteins Pathway Diagram
Selenium Pathway antibodies	Selenium Pathway Diagram
Glutathione Redox Reactions I Pathway antibodies	Glutathione Redox Reactions I Pathway Diagram
Glutathione Redox Reactions I Pathway antibodies	Glutathione Redox Reactions I Pathway Diagram

Diseases associated with GPX4 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>24 publications with GPX4 and Neoplasms
Drug Toxicity Antibodies	>12 publications with GPX4 and Drug Toxicity
Necrosis Antibodies	>8 publications with GPX4 and Necrosis
Breast Neoplasms Antibodies	>8 publications with GPX4 and Breast Neoplasms
Disease Models, Animal Antibodies	>7 publications with GPX4 and Disease Models, Animal
Gastrointestinal Diseases Antibodies	>7 publications with GPX4 and Gastrointestinal Diseases
Nervous System Diseases Antibodies	>6 publications with GPX4 and Nervous System Diseases
Inflammation Antibodies	>6 publications with GPX4 and Inflammation
Infertility, Male Antibodies	>6 publications with GPX4 and Infertility, Male
Liver Diseases Antibodies	>5 publications with GPX4 and Liver Diseases

Organs/Tissues associated with GPX4 elisa kit

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>41 publications with GPX4 and Liver
Blood Antibodies	>35 publications with GPX4 and Blood
Brain Antibodies	>21 publications with GPX4 and Brain
Testis Antibodies	>18 publications with GPX4 and Testis
Muscle Antibodies	>14 publications with GPX4 and Muscle
Lung Antibodies	>14 publications with GPX4 and Lung
Kidney Antibodies	>13 publications with GPX4 and Kidney
Heart Antibodies	>11 publications with GPX4 and Heart
Embryonic Tissue Antibodies	>9 publications with GPX4 and Embryonic Tissue
Vascular Antibodies	>6 publications with GPX4 and Vascular