Napsin A Aspartic Peptidase (NAPSA) ELISA Kit for Human

Name: Human NAPSA ELISA Kit; Napsin A Aspartic Peptidase; MyBioSource
Brand: MyBioSource
SKU: MBS908650
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS908650

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NAPSA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NAPSA present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

napsin A aspartic peptidase (NAPSA), ELISA Kit

Full Product Name

Human Napsin-A, NAPSA ELISA Kit

Product Gene Name

NAPSA elisa kit

Product Synonym Names

Human Napsin-A (NAPSA) ELISA kit; KAP; Kdap; NAP1; NAPA; SNAPA; napsin A; pronapsin A; napsin A aspartic peptidase

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates

Detection Range

4.7 ng/ml -300 ng/ml.

Sensitivity

The minimum detectable dose of human NAPSA is typically less than 1.18 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Specificity

This assay has high sensitivity and excellent specificity for detection of human NAPSA. No significant cross-reactivity or interference between human NAPSA and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

45,387 Da

OMIM

605631

3D Structure

ModBase 3D Structure for O96009

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of NAPSA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NAPSA purchase

MBS908650 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the napsin A aspartic peptidase (NAPSA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NAPSA. The ELISA analytical biochemical technique of the MBS908650 kit is based on NAPSA antibody-NAPSA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NAPSA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NAPSA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for NAPSA. It may not necessarily be applicable to this product.

NCBI GI #

4758754

NCBI GeneID

9476

NCBI Accession #

NP_004842.1

UniProt Primary Accession #

O96009

UniProt Secondary Accession #

Q8WWD9

UniProt Related Accession #

O96009

GenBank Nucleotide #

NM_004851.1

NCBI Official Full Name

napsin-A preproprotein

NCBI Official Synonym Full Names

napsin A aspartic peptidase

NCBI Official Symbol

NAPSA

NCBI Official Synonym Symbols

KAP; Kdap; NAP1; NAPA; SNAPA

UniProt Protein Name

Napsin-A

UniProt Synonym Protein Names

Aspartyl protease 4; ASP4; Asp 4; Napsin-1; TA01/TA02

Protein Family

Napsin

UniProt Gene Name

NAPSA

UniProt Synonym Gene Names

NAP1; NAPA; ASP4; Asp 4

UniProt Entry Name

NAPSA_HUMAN

NCBI Protein Information

napsin-A; ASP4; asp 4; napsin-1; TA01/TA02; pronapsin A; aspartyl protease 4; kidney-derived aspartic protease-like protein

NCBI Summary for NAPSA

The activation peptides of aspartic proteinases plays role as inhibitors of the active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The pronapsin A gene is expressed predominantly in lung and kidney. Its translation product is predicted to be a fully functional, glycosylated aspartic proteinase precursor containing an RGD motif and an additional 18 residues at its C-terminus. [provided by RefSeq, Jul 2008]

UniProt Comments for NAPSA

NAPSA: May be involved in processing of pneumocyte surfactant precursors. Belongs to the peptidase A1 family.

Protein type: Secreted; Secreted, signal peptide; EC 3.4.23.-; Protease

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: extracellular space; lysosome

Molecular Function: peptidase activity; endopeptidase activity; aspartic-type endopeptidase activity

Biological Process: membrane protein proteolysis; proteolysis; surfactant homeostasis

Research Articles on NAPSA

1. Napsin A as a marker of clear cell ovarian carcinoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with NAPSA elisa kit

Products by Pathway	Pathway Diagram
Lysosome Pathway antibodies	Lysosome Pathway Diagram
Lysosome Pathway antibodies	Lysosome Pathway Diagram

Diseases associated with NAPSA elisa kit

Disease Name	Pubmed Publications
Skin Diseases Antibodies	>3 publications with NAPSA and Skin Diseases
Fibrosis Antibodies	>2 publications with NAPSA and Fibrosis
Gastrointestinal Diseases Antibodies	>2 publications with NAPSA and Gastrointestinal Diseases
Pleural Effusion Antibodies	>2 publications with NAPSA and Pleural Effusion
Hyperplasia Antibodies	>1 publications with NAPSA and Hyperplasia
Mental Disorders Antibodies	>1 publications with NAPSA and Mental Disorders
Skin Neoplasms Antibodies	>1 publications with NAPSA and Skin Neoplasms
Pain Antibodies	>1 publications with NAPSA and Pain
Disease Models, Animal Antibodies	>1 publications with NAPSA and Disease Models, Animal
Acute Lung Injury Antibodies	>1 publications with NAPSA and Acute Lung Injury

Organs/Tissues associated with NAPSA elisa kit

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>31 publications with NAPSA and Lung
Kidney Antibodies	>12 publications with NAPSA and Kidney
Spleen Antibodies	>2 publications with NAPSA and Spleen
Prostate Antibodies	>1 publications with NAPSA and Prostate
Trachea Antibodies	>1 publications with NAPSA and Trachea