Vinculin (VCL) ELISA Kit for Human

Name: Human VCL ELISA Kit; Vinculin; MyBioSource
Brand: MyBioSource
SKU: MBS909803
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS909803

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VCL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VCL present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

vinculin (VCL), ELISA Kit

Full Product Name

Human Vinculin, VCL ELISA Kit

Product Gene Name

VCL elisa kit

Product Synonym Names

Human Vinculin (VCL) ELISA kit; RP11-178G16.3; CMD1W; MVCL; metavinculin; vinculin

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates, Cell lysates

Detection Range

28 pg/ml -1800 pg/ml.

Sensitivity

The minimum detectable dose of human VCL is typically less than 7 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Specificity

This assay has high sensitivity and excellent specificity for detection of human VCL. No significant cross-reactivity or interference between human VCL and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

123,799 Da

OMIM

613255

3D Structure

ModBase 3D Structure for P18206

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of VCL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VCL purchase

MBS909803 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the vinculin (VCL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing VCL. The ELISA analytical biochemical technique of the MBS909803 kit is based on VCL antibody-VCL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect VCL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, VCL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for VCL. It may not necessarily be applicable to this product.

NCBI GI #

4507877

NCBI GeneID

7414

NCBI Accession #

NP_003364.1

UniProt Primary Accession #

P18206

UniProt Secondary Accession #

Q16450; Q5SWX2; Q7Z3B8; Q8IXU7

UniProt Related Accession #

P18206

GenBank Nucleotide #

NM_003373.3

NCBI Official Full Name

vinculin isoform VCL

NCBI Official Synonym Full Names

vinculin

NCBI Official Symbol

VCL

NCBI Official Synonym Symbols

MV; MVCL; CMD1W; CMH15; HEL114

UniProt Protein Name

Vinculin

UniProt Synonym Protein Names

Metavinculin; MV

Protein Family

Vinculin

UniProt Gene Name

VCL

UniProt Synonym Gene Names

UniProt Entry Name

VINC_HUMAN

NCBI Protein Information

vinculin; metavinculin; epididymis luminal protein 114

NCBI Summary for VCL

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

UniProt Comments for VCL

Vinculin: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. Exhibits self-association properties. Interacts with NRAP and SORBS1. Interacts with TLN1. Interacts with SYNM. Interacts with CTNNB1 and this interaction is necessary for its localization to the cell-cell junctions and for its function in regulating cell surface expression of E-cadherin. Metavinculin is muscle-specific. Belongs to the vinculin/alpha-catenin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 10q22.2

Cellular Component: protein complex; focal adhesion; costamere; extracellular region; fascia adherens; intercellular junction; cytosol; cell-matrix junction; actin cytoskeleton; adherens junction; cell-cell adherens junction; cytoskeleton; plasma membrane; vesicle

Molecular Function: protein binding; cadherin binding; beta-catenin binding; actin binding; structural molecule activity; alpha-catenin binding

Biological Process: lamellipodium biogenesis; platelet activation; apical junction assembly; platelet degranulation; muscle contraction; axon extension; cell-matrix adhesion; morphogenesis of an epithelium; cell motility; cell adhesion; blood coagulation; negative regulation of cell migration

Disease: Cardiomyopathy, Dilated, 1w

Research Articles on VCL

1. Case Report: VCL-ALK gene fusion in renal cell carcinoma in patient with sickle cell trait.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with VCL elisa kit

Reference Product	PubMed Publications
PTK2 elisa kit	>7 publications with VCL and PTK2
SRC elisa kit	>6 publications with VCL and SRC
PXN elisa kit	>3 publications with VCL and PXN
CTNNA1 elisa kit	>2 publications with VCL and CTNNA1
ACTN1 elisa kit	>2 publications with VCL and ACTN1
PRKCA elisa kit	>1 publications with VCL and PRKCA
GSN elisa kit	>1 publications with VCL and GSN

Pathways associated with VCL elisa kit

Products by Pathway	Pathway Diagram
Adherens Junction Pathway antibodies	Adherens Junction Pathway Diagram
Adherens Junction Pathway antibodies	Adherens Junction Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Bacterial Invasion Of Epithelial Cells Pathway antibodies	Bacterial Invasion Of Epithelial Cells Pathway Diagram
Bacterial Invasion Of Epithelial Cells Pathway antibodies	Bacterial Invasion Of Epithelial Cells Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram

Diseases associated with VCL elisa kit

Disease Name	Pubmed Publications
Infertility, Male Antibodies	>40 publications with VCL and Infertility, Male
Neoplasms Antibodies	>25 publications with VCL and Neoplasms
Drug Toxicity Antibodies	>24 publications with VCL and Drug Toxicity
Brain Diseases Antibodies	>12 publications with VCL and Brain Diseases
Cardiomyopathy, Dilated Antibodies	>7 publications with VCL and Cardiomyopathy, Dilated
Kidney Diseases Antibodies	>6 publications with VCL and Kidney Diseases
Hypertension Antibodies	>6 publications with VCL and Hypertension
Neoplasm Metastasis Antibodies	>6 publications with VCL and Neoplasm Metastasis
Congenital Abnormalities Antibodies	>5 publications with VCL and Congenital Abnormalities
Liver Neoplasms Antibodies	>3 publications with VCL and Liver Neoplasms

Organs/Tissues associated with VCL elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>45 publications with VCL and Blood
Muscle Antibodies	>41 publications with VCL and Muscle
Vascular Antibodies	>19 publications with VCL and Vascular
Heart Antibodies	>15 publications with VCL and Heart
Testis Antibodies	>15 publications with VCL and Testis
Brain Antibodies	>14 publications with VCL and Brain
Lung Antibodies	>12 publications with VCL and Lung
Kidney Antibodies	>10 publications with VCL and Kidney
Liver Antibodies	>9 publications with VCL and Liver
Bone Antibodies	>8 publications with VCL and Bone