Hypocretin (Orexin) Neuropeptide Precursor (HCRT) ELISA Kit for Dog

Name: Dog HCRT ELISA Kit; Hypocretin (Orexin) Neuropeptide Precursor; MyBioSource
Brand: MyBioSource
SKU: MBS909932
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS909932

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HCRT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HCRT present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

hypocretin (orexin) neuropeptide precursor (HCRT), ELISA Kit

Full Product Name

Dog Orexin, HCRT ELISA Kit

Product Gene Name

HCRT elisa kit

Product Synonym Names

Dog Orexin (HCRT) ELISA kit; NRCLP1; OX; PPOX; orexin; hypocretin (orexin) neuropeptide precursor

Species Reactivity

Dog

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates

Detection Range

1.87 pg/ml -120 pg/ml.

Sensitivity

The minimum detectable dose of dog HCRT is typically less than 0.47 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Specificity

This assay has high sensitivity and excellent specificity for detection of dog HCRT. No significant cross-reactivity or interference between dog HCRT and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

13,328 Da

3D Structure

ModBase 3D Structure for Q9GLF6

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of HCRT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HCRT purchase

MBS909932 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the hypocretin (orexin) neuropeptide precursor (HCRT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HCRT. The ELISA analytical biochemical technique of the MBS909932 kit is based on HCRT antibody-HCRT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HCRT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HCRT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for HCRT. It may not necessarily be applicable to this product.

NCBI GI #

77404201

NCBI GeneID

607641

NCBI Accession #

NP_001029166.1

UniProt Primary Accession #

Q9GLF6

UniProt Related Accession #

Q9GLF6

GenBank Nucleotide #

NM_001033994.1

NCBI Official Full Name

orexin

NCBI Official Symbol

HCRT

UniProt Protein Name

Orexin

UniProt Synonym Protein Names

Hypocretin; Hcrt

Protein Family

Orexin

UniProt Gene Name

HCRT

UniProt Synonym Gene Names

OX; PPOX; Hcrt; Hcrt1; Hcrt2

UniProt Entry Name

OREX_CANFA

NCBI Protein Information

orexin

UniProt Comments for HCRT

Function: Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with HCRT elisa kit

Products by Pathway	Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
G Alpha (q) Signalling Events Pathway antibodies	G Alpha (q) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
Orexin And Neuropeptides FF And QRFP Bind To Their Respective Receptors Pathway antibodies	Orexin And Neuropeptides FF And QRFP Bind To Their Respective Receptors Pathway Diagram
Peptide Ligand-binding Receptors Pathway antibodies	Peptide Ligand-binding Receptors Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By GPCR Pathway antibodies	Signaling By GPCR Pathway Diagram

Diseases associated with HCRT elisa kit

Disease Name	Pubmed Publications
Narcolepsy Antibodies	>75 publications with HCRT and Narcolepsy
Disorders of Excessive Somnolence Antibodies	>37 publications with HCRT and Disorders of Excessive Somnolence
Disease Models, Animal Antibodies	>7 publications with HCRT and Disease Models, Animal
Bradycardia Antibodies	>6 publications with HCRT and Bradycardia
Cardiovascular Diseases Antibodies	>5 publications with HCRT and Cardiovascular Diseases
Lung Diseases Antibodies	>5 publications with HCRT and Lung Diseases
Immune System Diseases Antibodies	>4 publications with HCRT and Immune System Diseases
Intestinal Diseases Antibodies	>4 publications with HCRT and Intestinal Diseases
Heart Diseases Antibodies	>4 publications with HCRT and Heart Diseases
Drug Toxicity Antibodies	>4 publications with HCRT and Drug Toxicity