Indoleamine 2, 3-Dioxygenase 1 (IDO1) ELISA Kit for Mouse

Name: Mouse IDO1 ELISA Kit; Indoleamine 2, 3-Dioxygenase 1; MyBioSource
Brand: MyBioSource
SKU: MBS910999
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS910999

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IDO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IDO1 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

indoleamine 2, 3-dioxygenase 1 (IDO1), ELISA Kit

Full Product Name

Mouse Indoleamine 2, 3-dioxygenase 1, IDO1 ELISA Kit

Product Gene Name

IDO1 elisa kit

Product Synonym Names

Mouse Indoleamine 2; 3-dioxygenase 1 (IDO1) ELISA kit; CD107B; IDO; INDO; indole 2; 3-dioxygenase; indoleamine-pyrrole 2; 3 dioxygenase; indoleamine 2; 3-dioxygenase 1

Species Reactivity

Mouse

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates

Detection Range

62.5 pg/ml-4000 pg/ml

Sensitivity

< 15.6 pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of mouse IDO1. No significant cross-reactivity or interference between mouse IDO1 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

45,641 Da

3D Structure

ModBase 3D Structure for P28776

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of IDO1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IDO1 purchase

MBS910999 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the indoleamine 2, 3-dioxygenase 1 (IDO1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IDO1. The ELISA analytical biochemical technique of the MBS910999 kit is based on IDO1 antibody-IDO1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IDO1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IDO1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for IDO1. It may not necessarily be applicable to this product.

NCBI GI #

6680347

NCBI GeneID

15930

NCBI Accession #

NP_032350.1

UniProt Primary Accession #

P28776

UniProt Related Accession #

P28776

GenBank Nucleotide #

NM_008324.2

NCBI Official Full Name

indoleamine 2,3-dioxygenase 1 isoform 1

NCBI Official Synonym Full Names

indoleamine 2,3-dioxygenase 1

NCBI Official Symbol

Ido1

NCBI Official Synonym Symbols

Ido; Indo

UniProt Protein Name

Indoleamine 2,3-dioxygenase 1

UniProt Synonym Protein Names

Indoleamine-pyrrole 2,3-dioxygenase

Protein Family

Indoleamine 2,3-dioxygenase

UniProt Gene Name

Ido1

UniProt Synonym Gene Names

Ido; Indo; IDO-1

UniProt Entry Name

I23O1_MOUSE

NCBI Protein Information

indoleamine 2,3-dioxygenase 1; IDO-1; indole 2,3-dioxygenase; indoleamine-pyrrole 2,3 dioxygenase; indoleamine-pyrrole 2,3-dioxygenase

UniProt Comments for IDO1

INDO: Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. Belongs to the indoleamine 2,3-dioxygenase family.

Protein type: Cell cycle regulation; Amino Acid Metabolism - tryptophan; EC 1.13.11.52; Oxidoreductase

Cellular Component: smooth muscle contractile fiber; stereocilium bundle; cytoplasm; cytosol

Molecular Function: amino acid binding; protein binding; dioxygenase activity; metal ion binding; indoleamine 2,3-dioxygenase activity; heme binding; oxidoreductase activity; oxygen binding; tryptophan 2,3-dioxygenase activity

Biological Process: positive regulation of T-helper 2 type immune response; positive regulation of chronic inflammatory response; negative regulation of interleukin-10 production; tryptophan catabolic process to kynurenine; positive regulation of interleukin-12 production; positive regulation of apoptosis; negative regulation of activated T cell proliferation; response to lipopolysaccharide; defense response; negative regulation of T cell proliferation; cytokine production during acute inflammatory response; inflammatory response; multicellular organismal response to stress; tryptophan catabolic process; positive regulation of T cell tolerance induction

Research Articles on IDO1

1. Toxoplasma gondii infection induced high levels of HO-1 expression in lung of BALB/c and C57BL6 mice. Infected C57BL6 mice treated with hemin showed higher levels of indoleamine 2,3-dioxygenase expression in lung and small intestine than uninfected mice.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IDO1 elisa kit

Reference Product	PubMed Publications
Ifng elisa kit	>4 publications with IDO1 and Ifng
Il6 elisa kit	>3 publications with IDO1 and Il6
Kynu elisa kit	>2 publications with IDO1 and Kynu
Tph1 elisa kit	>1 publications with IDO1 and Tph1

Pathways associated with IDO1 elisa kit

Products by Pathway	Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Amino Acids And Derivatives Pathway antibodies	Metabolism Of Amino Acids And Derivatives Pathway Diagram
NAD Biosynthesis II (from Tryptophan) Pathway antibodies	NAD Biosynthesis II (from Tryptophan) Pathway Diagram
Tryptophan Catabolism Pathway antibodies	Tryptophan Catabolism Pathway Diagram
Tryptophan Metabolism Pathway antibodies	Tryptophan Metabolism Pathway Diagram
Tryptophan Metabolism Pathway antibodies	Tryptophan Metabolism Pathway Diagram
Tryptophan Metabolism Pathway antibodies	Tryptophan Metabolism Pathway Diagram

Diseases associated with IDO1 elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>36 publications with IDO1 and Inflammation
Disease Models, Animal Antibodies	>11 publications with IDO1 and Disease Models, Animal
Neoplasms, Experimental Antibodies	>9 publications with IDO1 and Neoplasms, Experimental
Colitis Antibodies	>6 publications with IDO1 and Colitis
Neurodegenerative Diseases Antibodies	>5 publications with IDO1 and Neurodegenerative Diseases
Necrosis Antibodies	>4 publications with IDO1 and Necrosis
Endometriosis Antibodies	>4 publications with IDO1 and Endometriosis
Lung Diseases Antibodies	>4 publications with IDO1 and Lung Diseases
Breast Neoplasms Antibodies	>4 publications with IDO1 and Breast Neoplasms
Colonic Neoplasms Antibodies	>2 publications with IDO1 and Colonic Neoplasms

Organs/Tissues associated with IDO1 elisa kit

Organ/Tissue Name	Pubmed Publications
Intestine Antibodies	>2 publications with IDO1 and Intestine
Epididymis Antibodies	>1 publications with IDO1 and Epididymis