R-Spondin 2 Homolog (Xenopus Laevis) (RSPO2) ELISA Kit for Human

Name: Human RSPO2 ELISA Kit; R-Spondin 2 Homolog (Xenopus Laevis); MyBioSource
Brand: MyBioSource
SKU: MBS911461
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS911461

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RSPO2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RSPO2 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

R-spondin 2 homolog (Xenopus laevis) (RSPO2), ELISA Kit

Full Product Name

Human R-spondin-2, RSPO2 ELISA Kit

Product Gene Name

RSPO2 elisa kit

Product Synonym Names

Human R-spondin-2 (RSPO2) ELISA kit; CRISTIN2; MGC35555; MGC43342; R-spondin family; member 2; R-spondin 2 homolog (Xenopus laevis)

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates, Cell lysates

Detection Range

0. 156 ng/ml -10 ng/ml.

Sensitivity

The minimum detectable dose of human RSPO2 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Specificity

This assay has high sensitivity and excellent specificity for detection of human RSPO2. No significant cross-reactivity or interference between human RSPO2 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

28,315 Da

OMIM

610575

3D Structure

ModBase 3D Structure for Q6UXX9

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of RSPO2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RSPO2 purchase

MBS911461 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the R-spondin 2 homolog (Xenopus laevis) (RSPO2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RSPO2. The ELISA analytical biochemical technique of the MBS911461 kit is based on RSPO2 antibody-RSPO2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RSPO2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RSPO2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for RSPO2. It may not necessarily be applicable to this product.

NCBI GI #

544583460

NCBI GeneID

340419

NCBI Accession #

NP_001269792.1

UniProt Primary Accession #

Q6UXX9

UniProt Secondary Accession #

Q4G0U4; Q8N6X6; B3KVP0

UniProt Related Accession #

Q6UXX9

GenBank Nucleotide #

NM_001282863.1

NCBI Official Full Name

R-spondin-2 isoform 2

NCBI Official Synonym Full Names

R-spondin 2

NCBI Official Symbol

RSPO2

NCBI Official Synonym Symbols

CRISTIN2

UniProt Protein Name

R-spondin-2

UniProt Synonym Protein Names

Roof plate-specific spondin-2; hRspo2

Protein Family

R-spondin

UniProt Gene Name

RSPO2

UniProt Synonym Gene Names

UNQ9384/PRO34209; hRspo2

UniProt Entry Name

RSPO2_HUMAN

NCBI Protein Information

R-spondin-2; R-spondin 2 homolog; roof plate-specific spondin-2

NCBI Summary for RSPO2

R-spondins (RSPOs), such as RSPO2, are secreted proteins that regulate beta-catenin (CTNNB1; MIM 116806) signaling (Kim et al., 2006 [PubMed 16357527]).[supplied by OMIM, Mar 2008]

UniProt Comments for RSPO2

R-spondin-2: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Probably also acts as a ligand for frizzled and LRP receptors. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Cell development/differentiation; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8q23.1

Cellular Component: extracellular space; cell surface; extracellular region

Molecular Function: heparin binding; G-protein-coupled receptor binding; receptor binding

Biological Process: embryonic forelimb morphogenesis; osteoblast differentiation; Wnt receptor signaling pathway; embryonic hindlimb morphogenesis; bone mineralization

Research Articles on RSPO2

1. Oocyte-derived R-spondin2 is a paracrine factor essential for primary ovarian follicle development

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with RSPO2 elisa kit

Disease Name	Pubmed Publications
Congenital Abnormalities Antibodies	>5 publications with RSPO2 and Congenital Abnormalities
Neoplasms Antibodies	>3 publications with RSPO2 and Neoplasms
Craniofacial Abnormalities Antibodies	>2 publications with RSPO2 and Craniofacial Abnormalities
Lung Neoplasms Antibodies	>1 publications with RSPO2 and Lung Neoplasms
Nervous System Diseases Antibodies	>1 publications with RSPO2 and Nervous System Diseases
Fibrosis Antibodies	>1 publications with RSPO2 and Fibrosis
Carcinoma Antibodies	>1 publications with RSPO2 and Carcinoma
Cleft Palate Antibodies	>1 publications with RSPO2 and Cleft Palate
Jaw Abnormalities Antibodies	>1 publications with RSPO2 and Jaw Abnormalities
Gonadal Disorders Antibodies	>1 publications with RSPO2 and Gonadal Disorders

Organs/Tissues associated with RSPO2 elisa kit

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>3 publications with RSPO2 and Lung
Embryonic Tissue Antibodies	>2 publications with RSPO2 and Embryonic Tissue
Blood Antibodies	>2 publications with RSPO2 and Blood
Muscle Antibodies	>2 publications with RSPO2 and Muscle
Skin Antibodies	>1 publications with RSPO2 and Skin
Testis Antibodies	>1 publications with RSPO2 and Testis
Intestine Antibodies	>1 publications with RSPO2 and Intestine
Brain Antibodies	>1 publications with RSPO2 and Brain