Guanylate Cyclase Activator 2b (Uroguanylin) (GUCA2B) ELISA Kit for Mouse

Name: Mouse GUCA2B ELISA Kit; Guanylate Cyclase Activator 2b (Uroguanylin); MyBioSource
Brand: MyBioSource
SKU: MBS912299
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS912299

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GUCA2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GUCA2B present is bound by the… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

guanylate cyclase activator 2B (uroguanylin) (GUCA2B), ELISA Kit

Full Product Name

Mouse guanylate cyclase activator 2B (uroguanylin) , GUCA2B ELISA Kit

Product Gene Name

GUCA2B elisa kit

Product Synonym Names

Mouse guanylate cyclase activator 2B (uroguanylin) (GUCA2B) ELISA kit; GCAP-II; UGN; guanylate cyclase activator 2B; uroguanylin; guanylate cyclase activator 2B (uroguanylin)

Species Reactivity

Mouse

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates

Detection Range

62.5 ng/ml-4000 ng/ml

Sensitivity

< 15.6 ng/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of mouse GUCA2B. No significant cross-reactivity or interference between mouse GUCA2B and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%.Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

11,628 Da

3D Structure

ModBase 3D Structure for O09051

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of GUCA2B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GUCA2B purchase

MBS912299 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the guanylate cyclase activator 2B (uroguanylin) (GUCA2B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GUCA2B. The ELISA analytical biochemical technique of the MBS912299 kit is based on GUCA2B antibody-GUCA2B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GUCA2B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GUCA2B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GUCA2B. It may not necessarily be applicable to this product.

NCBI GI #

160298221

NCBI GeneID

14916

NCBI Accession #

NP_032217.2

UniProt Primary Accession #

O09051

UniProt Related Accession #

O09051

GenBank Nucleotide #

NM_008191.2

NCBI Official Full Name

guanylate cyclase activator 2B preproprotein

NCBI Official Synonym Full Names

guanylate cyclase activator 2b (retina)

NCBI Official Symbol

Guca2b

NCBI Official Synonym Symbols

Ugn; Gcap2; AV066530

UniProt Protein Name

Guanylate cyclase activator 2B

Protein Family

Guanylate cyclase activator

UniProt Gene Name

Guca2b

UniProt Synonym Gene Names

UGN

UniProt Entry Name

GUC2B_MOUSE

NCBI Protein Information

guanylate cyclase activator 2B; uroguanylin; granule cell antiserum positive 2

NCBI Summary for GUCA2B

This gene encodes a member of the guanylin family and preproprotein that is proteolytically processed to generate a mature protein product. The mature protein product, known as uroguanylin, is an endogenous ligand for the guanylate cyclase-C receptor and may regulate salt and water homeostasis in the intestine and kidneys. Homozygous knockout mice for this gene exhibit impaired sodium chloride excretion and elevated arterial pressure. This gene is present in a gene cluster with a related guanylin family member on chromosome 4. [provided by RefSeq, Sep 2015]

UniProt Comments for GUCA2B

GUCA2B: Endogenous activator of intestinal guanylate cyclase. It stimulates this enzyme through the same receptor binding region as the heat-stable enterotoxins. May be a potent physiological regulator of intestinal fluid and electrolyte transport. May be an autocrine/paracrine regulator of intestinal salt and water transport. Belongs to the guanylin family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: photoreceptor outer segment; extracellular region

Molecular Function: guanylate cyclase activator activity

Biological Process: cGMP biosynthetic process; regulation of cyclic nucleotide biosynthetic process; negative regulation of blood pressure; excretion; body fluid secretion

Research Articles on GUCA2B

1. UGN exerts its inhibitory activity on the prendrin gene promoter likely via heat shock factor 1 action at a defined heat shock element site.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with GUCA2B elisa kit

Products by Pathway	Pathway Diagram
Myometrial Relaxation And Contraction Pathways antibodies	Myometrial Relaxation And Contraction Pathways Diagram

Diseases associated with GUCA2B elisa kit

Disease Name	Pubmed Publications
Necrosis Antibodies	>1 publications with GUCA2B and Necrosis
Inflammation Antibodies	>1 publications with GUCA2B and Inflammation
Neoplasms Antibodies	>1 publications with GUCA2B and Neoplasms
Hypertension Antibodies	>1 publications with GUCA2B and Hypertension
Cardiovascular Diseases Antibodies	>1 publications with GUCA2B and Cardiovascular Diseases

Organs/Tissues associated with GUCA2B elisa kit

Organ/Tissue Name	Pubmed Publications
Uterus Antibodies	>1 publications with GUCA2B and Uterus
Embryonic Tissue Antibodies	>1 publications with GUCA2B and Embryonic Tissue
Intestine Antibodies	>1 publications with GUCA2B and Intestine