Tumor Protein, Translationally-Controlled 1 (TPT1) ELISA Kit for Rat

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TPT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of TPT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS912958 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the tumor protein, translationally-controlled 1 (TPT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TPT1. The ELISA analytical biochemical technique of the MBS912958 kit is based on TPT1 antibody-TPT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TPT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TPT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Although translationally controlled tumor protein TPT1 was reported nearly 25 years ago, limited functional and cell expression data are known. Understanding more about this important histamine interacting protein will be aided by a precision rat specific TPT1 protein ELISA kit assay. Designed with speed, ease of performance and high reproducibility, this sandwich format TPT1 ELISA utilizes a prebound antibody 96 tests 1 plate and quality protein translationally controlled 1 TPT1 recombinant protein for standard curve quantitation - ideal for IHC comparison studies. This rat TPT and human / mouse ELISA kits are validated for multiple samples and have intra assay below _8% and detection ranges of 39 pg ml - 20ng ml. MyBiosource is offering this product order at promotional pricing for limited days, inquire about this and related human tumor necrosis factor products today to receive this deal.