Chitinase 3-Like 1 (Cartilage Glycoprotein-39) (CHI3L1) ELISA Kit for Rat

Name: Rat CHI3L1 ELISA Kit; Chitinase 3-Like 1 (Cartilage Glycoprotein-39); MyBioSource
Brand: MyBioSource
SKU: MBS914441
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS914441

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CHI3L1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHI3L1 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

chitinase 3-like 1 (cartilage glycoprotein-39) (CHI3L1), ELISA Kit

Full Product Name

Rat Chitinase-3-like protein 1, CHI3L1 ELISA Kit

Product Gene Name

CHI3L1 elisa kit

Product Synonym Names

Rat Chitinase-3-like protein 1 (CHI3L1) ELISA kit; ASRT7; DKFZp686N19119; FLJ38139; GP39; HC-gp39; HCGP-3P; YKL40; YYL-40; chitinase 3-like 1; chitinase 3-like 1 (cartilage glycoprotein-39)

Species Reactivity

Rat

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates

Detection Range

4.7 ng/ml-300 ng/ml

Sensitivity

< 1.17 ng/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of rat CHI3L1. No significant cross-reactivity or interference between rat CHI3L1 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

42,392 Da

3D Structure

ModBase 3D Structure for Q9WTV1

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CHI3L1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CHI3L1 purchase

MBS914441 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the chitinase 3-like 1 (cartilage glycoprotein-39) (CHI3L1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CHI3L1. The ELISA analytical biochemical technique of the MBS914441 kit is based on CHI3L1 antibody-CHI3L1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CHI3L1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CHI3L1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CHI3L1. It may not necessarily be applicable to this product.

NCBI GI #

61657901

NCBI GeneID

89824

NCBI Accession #

NP_446012.1

UniProt Primary Accession #

Q9WTV1

UniProt Secondary Accession #

Q5BJR6

UniProt Related Accession #

Q9WTV1

GenBank Nucleotide #

NM_053560.1

NCBI Official Full Name

chitinase-3-like protein 1

NCBI Official Synonym Full Names

chitinase 3-like 1 (cartilage glycoprotein-39)

NCBI Official Symbol

Chi3l1

NCBI Official Synonym Symbols

Chil1

UniProt Protein Name

Chitinase-3-like protein 1

UniProt Synonym Protein Names

Cartilage glycoprotein 39; CGP-39; GP-39

Protein Family

Chitinase-3-like protein

UniProt Gene Name

Chi3l1

UniProt Synonym Gene Names

CGP-39; GP-39

UniProt Entry Name

CH3L1_RAT

NCBI Protein Information

chitinase-3-like protein 1; GP-39; CGP-39; cartilage glycoprotein 39; chitinase 3-like 1 protein

NCBI Summary for CHI3L1

human homolog plays a role in vascular smooth muscle cell migration and adhesion [RGD, Feb 2006]

UniProt Comments for CHI3L1

Function: Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung

Research Articles on CHI3L1

1. Data show that hepatic CD14+ cells are an YKL-40(Chi3L1)mRNA and protein source in acute and chronic liver injury, with expression patterns similar to growth factors implicated in inflammation-fibrogenesis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with CHI3L1 elisa kit

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Diseases associated with CHI3L1 elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>116 publications with CHI3L1 and Inflammation
Neoplasms Antibodies	>108 publications with CHI3L1 and Neoplasms
Lung Diseases Antibodies	>47 publications with CHI3L1 and Lung Diseases
Fibrosis Antibodies	>43 publications with CHI3L1 and Fibrosis
Nervous System Diseases Antibodies	>37 publications with CHI3L1 and Nervous System Diseases
Brain Diseases Antibodies	>34 publications with CHI3L1 and Brain Diseases
Skin Diseases Antibodies	>32 publications with CHI3L1 and Skin Diseases
Glioma Antibodies	>32 publications with CHI3L1 and Glioma
Liver Diseases Antibodies	>28 publications with CHI3L1 and Liver Diseases
Hypersensitivity Antibodies	>27 publications with CHI3L1 and Hypersensitivity

Organs/Tissues associated with CHI3L1 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>47 publications with CHI3L1 and Brain
Lung Antibodies	>43 publications with CHI3L1 and Lung
Connective Tissue Antibodies	>43 publications with CHI3L1 and Connective Tissue
Spleen Antibodies	>4 publications with CHI3L1 and Spleen