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Nodal Homolog (Mouse) (NODAL) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS915258

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NODAL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NODAL present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
nodal homolog (mouse), ELISA Kit
Full Product Name
Human Nodal homolog, NODAL ELISA Kit
Product Gene Name
NODAL elisa kit
Product Synonym Names
Human Nodal homolog (NODAL) ELISA kit; MGC138230; nodal; nodal homolog (mouse)
Species Reactivity
Human
Samples
Serum, plasma, tissue homogenates
Detection Range
78 pg/ml -5000 pg/mL
Sensitivity
<19.5 pg/mL
Specificity
This assay has high sensitivity and excellent specificity for detection of human NODAL. No significant cross-reactivity or interference between human NODAL and analogues was observed.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Molecular Weight
39,561 Da
OMIM
601265
3D Structure
ModBase 3D Structure for Q96S42
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of NODAL elisa kit

Human NODAL ELISA Kit – Cat# MBS915258

Other Notes

Small volumes of NODAL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NODAL purchase

MBS915258 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the nodal homolog (mouse) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NODAL. The ELISA analytical biochemical technique of the MBS915258 kit is based on NODAL antibody-NODAL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NODAL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NODAL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for NODAL. It may not necessarily be applicable to this product.

NCBI GI #
222352098
NCBI GeneID
4838
NCBI Accession #
NP_060525.3
UniProt Primary Accession #
Q96S42
UniProt Secondary Accession #
Q2M3A5; Q8N4V3
UniProt Related Accession #
Q96S42
GenBank Nucleotide #
NM_018055.4
NCBI Official Full Name
nodal homolog
NCBI Official Synonym Full Names
nodal growth differentiation factor
NCBI Official Symbol
NODAL
NCBI Official Synonym Symbols
HTX5
UniProt Protein Name
Nodal homolog
Protein Family
Nodal
UniProt Gene Name
NODAL
UniProt Entry Name
NODAL_HUMAN
NCBI Protein Information
nodal homolog

NCBI Summary for NODAL

The protein encoded by this gene is a member of the TGF-beta superfamily. Studies of the mouse counterpart suggested that this gene may be essential for mesoderm formation and subsequent organization of axial structures in early embryonic development. [provided by RefSeq, Jul 2008]

UniProt Comments for NODAL

NODAL: Essential for mesoderm formation and axial patterning during embryonic development. Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5). A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia. Belongs to the TGF-beta family.

Protein type: Cytokine; Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted; Cell development/differentiation

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: extracellular space

Molecular Function: morphogen activity; growth factor activity; cytokine activity; transforming growth factor beta receptor binding; receptor agonist activity

Biological Process: embryonic placenta development; mesendoderm development; positive regulation of caspase activity; axial mesodermal cell fate specification; negative regulation of transcription from RNA polymerase II promoter; embryonic pattern specification; positive regulation of activin receptor signaling pathway; floor plate morphogenesis; vasculature development; regulation of gastrulation; positive regulation of cell-cell adhesion; trophectodermal cellular morphogenesis; heart looping; cell migration involved in gastrulation; maternal placenta development; placenta development; stem cell maintenance; embryonic process involved in female pregnancy; digestive tract morphogenesis; embryonic cranial skeleton morphogenesis; liver development; formation of anatomical boundary; positive regulation of angiogenesis; neural fold formation; repression of premature neural plate formation; maternal process involved in parturition; polarity specification of proximal/distal axis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; brain development; positive regulation of epithelial cell proliferation; growth

Disease: Heterotaxy, Visceral, 5, Autosomal
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Research Articles on NODAL

1. Nodal regulates energy metabolism in glioma cells by inducing expression of hypoxia-inducible factor 1alpha.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with NODAL elisa kit

 Products by Pathway  Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Regulation Of Signaling By NODAL Pathway antibodies  Regulation Of Signaling By NODAL Pathway Diagram
 Signaling By NODAL Pathway antibodies  Signaling By NODAL Pathway Diagram
 TGF-beta Signaling Pathway antibodies  TGF-beta Signaling Pathway Diagram
 TGF-beta Signaling Pathway antibodies  TGF-beta Signaling Pathway Diagram

Diseases associated with NODAL elisa kit

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >17317 publications with NODAL and Neoplasms
 Carcinoma Antibodies  >9317 publications with NODAL and Carcinoma
 Neoplasm Metastasis Antibodies  >8543 publications with NODAL and Neoplasm Metastasis
 Cardiovascular Diseases Antibodies  >5224 publications with NODAL and Cardiovascular Diseases
 Heart Diseases Antibodies  >5024 publications with NODAL and Heart Diseases
 Arrhythmias, Cardiac Antibodies  >4785 publications with NODAL and Arrhythmias, Cardiac
 Breast Neoplasms Antibodies  >3742 publications with NODAL and Breast Neoplasms
 Adenocarcinoma Antibodies  >3496 publications with NODAL and Adenocarcinoma
 Carcinoma, Squamous Cell Antibodies  >2904 publications with NODAL and Carcinoma, Squamous Cell
 Lung Neoplasms Antibodies  >1899 publications with NODAL and Lung Neoplasms

Organs/Tissues associated with NODAL elisa kit

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >618 publications with NODAL and Brain
 Embryonic Tissue Antibodies  >395 publications with NODAL and Embryonic Tissue
 Connective Tissue Antibodies  >181 publications with NODAL and Connective Tissue
 Testis Antibodies  >125 publications with NODAL and Testis
 Placenta Antibodies  >36 publications with NODAL and Placenta
 Uncharacterized Tissue Antibodies  >3 publications with NODAL and Uncharacterized Tissue
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