Serum/Glucocorticoid Regulated Kinase 1 (SGK1) ELISA Kit for Human

Name: Human SGK1 ELISA Kit; Serum/Glucocorticoid Regulated Kinase 1; MyBioSource
Brand: MyBioSource
SKU: MBS915643
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS915643

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SGK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SGK1 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

serum/glucocorticoid regulated kinase 1 (SGK1), ELISA Kit

Full Product Name

Human Serine/threonine-protein kinase Sgk1, SGK1 ELISA Kit

Product Gene Name

SGK1 elisa kit

Product Synonym Names

Human Serine/threonine-protein kinase Sgk1 (SGK1) ELISA kit; RP1-188K17.1; SGK; serine/threonine protein kinase SGK; serum/glucocorticoid regulated kinase 1

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates, cell lysates

Detection Range

23.44 pg/ml-1500 pg/ml

Sensitivity

< 5.86 pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of human SGK1. No significant cross-reactivity or interference between human SGK1 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%.Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

48,942 Da

OMIM

602958

3D Structure

ModBase 3D Structure for O00141

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of SGK1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SGK1 purchase

MBS915643 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the serum/glucocorticoid regulated kinase 1 (SGK1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SGK1. The ELISA analytical biochemical technique of the MBS915643 kit is based on SGK1 antibody-SGK1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SGK1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SGK1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for SGK1. It may not necessarily be applicable to this product.

NCBI GI #

219521876

NCBI GeneID

6446

NCBI Accession #

NP_001137148.1

UniProt Primary Accession #

O00141

UniProt Secondary Accession #

Q5TCN2; Q5TCN3; Q5TCN4; Q5VY65; Q9UN56; B7UUP7; B7UUP8; B7UUP9; B7Z5B2; E1P583

UniProt Related Accession #

O00141

GenBank Nucleotide #

NM_001143676.1

NCBI Official Full Name

serine/threonine-protein kinase Sgk1 isoform 2

NCBI Official Synonym Full Names

serum/glucocorticoid regulated kinase 1

NCBI Official Symbol

SGK1

NCBI Official Synonym Symbols

SGK

UniProt Protein Name

Serine/threonine-protein kinase Sgk1

UniProt Synonym Protein Names

Serum/glucocorticoid-regulated kinase 1

Protein Family

Serine/threonine-protein kinase

UniProt Gene Name

SGK1

UniProt Synonym Gene Names

SGK

UniProt Entry Name

SGK1_HUMAN

NCBI Protein Information

serine/threonine-protein kinase Sgk1; Sgk1 variant i3; serine/threonine protein kinase SGK; serum/glucocorticoid-regulated kinase 1

NCBI Summary for SGK1

This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jan 2009]

UniProt Comments for SGK1

SGK1: serum and glucocorticoid-inducible kinase is an AGC protein kinase of the SGK family. Rapidly induced in response to a variety of stimuli including serum, glucocorticoids, follicle stimulating hormone, osmotic shock and mineralocorticoids. Can be activated via PI3K-dependent and -independent pathways. Plays a role in activating certain potassium, sodium and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion. SGK is negatively regulated by ubiquitin modification and proteasome degradation.

Protein type: Motility/polarity/chemotaxis; Kinase, protein; EC 2.7.11.1; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); AGC group; SGK family

Chromosomal Location of Human Ortholog: 6q23

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; mitochondrion; cytoplasm; plasma membrane; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; potassium channel regulator activity; chloride channel regulator activity; sodium channel regulator activity; calcium channel regulator activity; protein serine/threonine/tyrosine kinase activity; ATP binding

Biological Process: apoptosis; positive regulation of transporter activity; sodium ion transport; protein amino acid phosphorylation; regulation of cell migration; regulation of cell proliferation; regulation of apoptosis; regulation of transcription factor activity; long-term memory; regulation of catalytic activity; regulation of blood pressure; response to stress; regulation of cell growth; transmembrane transport; response to DNA damage stimulus

Research Articles on SGK1

1. Orai1 is expressed in the human endometrium and is up-regulated by SGK1 and TGFbeta1.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SGK1 elisa kit

Reference Product	PubMed Publications
NEDD4 elisa kit	>74 publications with SGK1 and NEDD4
CTNNB1 elisa kit	>14 publications with SGK1 and CTNNB1
KCNJ1 elisa kit	>9 publications with SGK1 and KCNJ1
FOXO3 elisa kit	>6 publications with SGK1 and FOXO3
NEDD4L elisa kit	>3 publications with SGK1 and NEDD4L
PDK2 elisa kit	>2 publications with SGK1 and PDK2
SLC9A3R2 elisa kit	>2 publications with SGK1 and SLC9A3R2
NEK6 elisa kit	>1 publications with SGK1 and NEK6
GSK3A elisa kit	>1 publications with SGK1 and GSK3A
CREB1 elisa kit	>1 publications with SGK1 and CREB1

Pathways associated with SGK1 elisa kit

Products by Pathway	Pathway Diagram
Aldosterone-regulated Sodium Reabsorption Pathway antibodies	Aldosterone-regulated Sodium Reabsorption Pathway Diagram
Aldosterone-regulated Sodium Reabsorption Pathway antibodies	Aldosterone-regulated Sodium Reabsorption Pathway Diagram
Class I PI3K Signaling Events Pathway antibodies	Class I PI3K Signaling Events Pathway Diagram
FoxO Family Signaling Pathway antibodies	FoxO Family Signaling Pathway Diagram
Glucocorticoid Receptor Regulatory Network Pathway antibodies	Glucocorticoid Receptor Regulatory Network Pathway Diagram
IL-6 Signaling Pathway antibodies	IL-6 Signaling Pathway Diagram
Insulin Pathway antibodies	Insulin Pathway Diagram
Insulin Signaling Pathway antibodies	Insulin Signaling Pathway Diagram
MTOR Signaling Pathway antibodies	MTOR Signaling Pathway Diagram

Diseases associated with SGK1 elisa kit

Disease Name	Pubmed Publications
Hypertension Antibodies	>58 publications with SGK1 and Hypertension
Cardiovascular Diseases Antibodies	>36 publications with SGK1 and Cardiovascular Diseases
Kidney Diseases Antibodies	>34 publications with SGK1 and Kidney Diseases
Fibrosis Antibodies	>34 publications with SGK1 and Fibrosis
Vascular Diseases Antibodies	>30 publications with SGK1 and Vascular Diseases
Neoplasms Antibodies	>29 publications with SGK1 and Neoplasms
Disease Models, Animal Antibodies	>21 publications with SGK1 and Disease Models, Animal
Inflammation Antibodies	>15 publications with SGK1 and Inflammation
Proteinuria Antibodies	>14 publications with SGK1 and Proteinuria
Nervous System Diseases Antibodies	>11 publications with SGK1 and Nervous System Diseases

Organs/Tissues associated with SGK1 elisa kit

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>169 publications with SGK1 and Kidney
Blood Antibodies	>112 publications with SGK1 and Blood
Lung Antibodies	>32 publications with SGK1 and Lung
Brain Antibodies	>30 publications with SGK1 and Brain
Heart Antibodies	>29 publications with SGK1 and Heart
Muscle Antibodies	>26 publications with SGK1 and Muscle
Intestine Antibodies	>19 publications with SGK1 and Intestine
Liver Antibodies	>12 publications with SGK1 and Liver
Bone Antibodies	>11 publications with SGK1 and Bone
Vascular Antibodies	>9 publications with SGK1 and Vascular