EGF-Containing Fibulin-Like Extracellular Matrix Protein 1 (EFEMP1) ELISA Kit for Human

Name: Human EFEMP1 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS915805
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS915805

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EFEMP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EFEMP1 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1), ELISA Kit

Full Product Name

Human EGF-containing fibulin-like extracellular matrix protein 1, EFEMP1 ELISA Kit

Product Gene Name

EFEMP1 elisa kit

Product Synonym Names

Human EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1) ELISA kit; DHRD; DRAD; FBLN3; FBNL; FLJ35535; MGC111353; MLVT; MTLV; S1-5; fibrillin-like; fibulin 3; EGF-containing fibulin-like extracellular matrix protein 1

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates, cell lysates

Detection Range

1.56 ng/ml-100 ng/ml

Sensitivity

< 0.39 ng/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of human EFEMP1. No significant cross-reactivity or interference between human EFEMP1 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

54,641 Da

OMIM

601548

3D Structure

ModBase 3D Structure for Q12805

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of EFEMP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EFEMP1 purchase

MBS915805 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EFEMP1. The ELISA analytical biochemical technique of the MBS915805 kit is based on EFEMP1 antibody-EFEMP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EFEMP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EFEMP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for EFEMP1. It may not necessarily be applicable to this product.

NCBI GI #

86788015

NCBI GeneID

2202

NCBI Accession #

NP_001034437.1

UniProt Primary Accession #

Q12805

UniProt Secondary Accession #

Q541U7; A8K3I4; B4DW75; D6W5D2

UniProt Related Accession #

Q12805

GenBank Nucleotide #

NM_001039348.2

NCBI Official Full Name

EGF-containing fibulin-like extracellular matrix protein 1

NCBI Official Synonym Full Names

EGF containing fibulin-like extracellular matrix protein 1

NCBI Official Symbol

EFEMP1

NCBI Official Synonym Symbols

DHRD; DRAD; FBNL; MLVT; MTLV; S1-5; FBLN3; FIBL-3

UniProt Protein Name

EGF-containing fibulin-like extracellular matrix protein 1

UniProt Synonym Protein Names

Extracellular protein S1-5; Fibrillin-like protein; Fibulin-3; FIBL-3

Protein Family

EGF-containing fibulin-like extracellular matrix protein

UniProt Gene Name

EFEMP1

UniProt Synonym Gene Names

FBLN3; FBNL; FIBL-3

UniProt Entry Name

FBLN3_HUMAN

NCBI Protein Information

EGF-containing fibulin-like extracellular matrix protein 1; fibulin-3; extracellular protein S1-5

NCBI Summary for EFEMP1

This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.[provided by RefSeq, Nov 2009]

UniProt Comments for EFEMP1

EFEMP1: Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth. Defects in EFEMP1 are a cause of Doyne honeycomb retinal dystrophy (DHRD); also known as malattia leventinese (MLVT) (ML). DHRD is an autosomal dominant disease characterized by yellow-white deposits known as drusen that accumulate beneath the retinal pigment epithelium. Belongs to the fibulin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2p16

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: protein binding; growth factor activity; epidermal growth factor receptor activity; calcium ion binding; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; extracellular matrix organization and biogenesis; peptidyl-tyrosine phosphorylation; visual perception; regulation of transcription, DNA-dependent; negative regulation of chondrocyte differentiation

Disease: Doyne Honeycomb Retinal Dystrophy

Research Articles on EFEMP1

1. Subtype-independent loss of EFEMP1 expression in the majority of primary adenomas should prompt more detailed investigation in this tumor type.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with EFEMP1 elisa kit

Reference Product	PubMed Publications
TIMP3 elisa kit	>7 publications with EFEMP1 and TIMP3

Diseases associated with EFEMP1 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>19 publications with EFEMP1 and Neoplasms
Doyne honeycomb retinal dystrophy Antibodies	>14 publications with EFEMP1 and Doyne honeycomb retinal dystrophy
Carcinoma Antibodies	>12 publications with EFEMP1 and Carcinoma
Adenocarcinoma Antibodies	>5 publications with EFEMP1 and Adenocarcinoma
Lung Neoplasms Antibodies	>4 publications with EFEMP1 and Lung Neoplasms
Hepatitis Antibodies	>3 publications with EFEMP1 and Hepatitis
Disease Models, Animal Antibodies	>3 publications with EFEMP1 and Disease Models, Animal
Neoplasms, Experimental Antibodies	>3 publications with EFEMP1 and Neoplasms, Experimental
Carcinoma, Hepatocellular Antibodies	>2 publications with EFEMP1 and Carcinoma, Hepatocellular
Ovarian Neoplasms Antibodies	>2 publications with EFEMP1 and Ovarian Neoplasms

Organs/Tissues associated with EFEMP1 elisa kit

Organ/Tissue Name	Pubmed Publications
Eye Antibodies	>29 publications with EFEMP1 and Eye
Vascular Antibodies	>12 publications with EFEMP1 and Vascular
Blood Antibodies	>11 publications with EFEMP1 and Blood
Nerve Antibodies	>7 publications with EFEMP1 and Nerve
Brain Antibodies	>7 publications with EFEMP1 and Brain
Lymph Node Antibodies	>5 publications with EFEMP1 and Lymph Node
Connective Tissue Antibodies	>5 publications with EFEMP1 and Connective Tissue
Lung Antibodies	>4 publications with EFEMP1 and Lung
Bone Antibodies	>4 publications with EFEMP1 and Bone
Liver Antibodies	>3 publications with EFEMP1 and Liver