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Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS916526

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LGALS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LGALS1 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
lectin, galactoside-binding, soluble, 1 (LGALS1), ELISA Kit
Full Product Name
Mouse Galectin-1, LGALS1 ELISA Kit
Product Gene Name
Product Synonym Names
Mouse Galectin-1 (LGALS1) ELISA kit; DKFZp686E23103; GAL1; GBP; S-Lac lectin 1; beta-galactoside-binding lectin L-14-I; galaptin; galectin 1; galectin-1; lactose-binding lectin 1; lectin; galactoside-binding; soluble; 1
Species Reactivity
Mouse
Assay Type
Sandwich
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse LGALS1. No significant cross-reactivity or interference between Mouse LGALS1 and analogues was observed.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Molecular Weight
14,866 Da
3D Structure
ModBase 3D Structure for P16045
Detection Wavelength
450 nm
Sample Volume
50-100ul
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of LGALS1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LGALS1 purchase

MBS916526 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the lectin, galactoside-binding, soluble, 1 (LGALS1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LGALS1. The ELISA analytical biochemical technique of the MBS916526 kit is based on LGALS1 antibody-LGALS1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LGALS1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LGALS1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for LGALS1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
galectin-1
NCBI Official Synonym Full Names
lectin, galactose binding, soluble 1
NCBI Official Symbol
Lgals1
NCBI Official Synonym Symbols
L14; Gal-1; Galbp; L-14.5; Lect14; AA410090; galectin-1
UniProt Protein Name
Galectin-1
UniProt Synonym Protein Names
14 kDa lectin; Beta-galactoside-binding lectin L-14-I; Galaptin; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; S-Lac lectin 1
UniProt Gene Name
Lgals1
UniProt Synonym Gene Names
Gbp; Gal-1
UniProt Entry Name
LEG1_MOUSE
NCBI Protein Information
galectin-1; galaptin; 14 kDa lectin; S-Lac lectin 1; lactose-binding lectin 1; beta-galactoside binding protein; lectin galactoside-binding soluble 1; beta-galactoside-binding lectin L-14-I

UniProt Comments for LGALS1

Galectin-1: May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Homodimer. Binds LGALS3BP. Interacts with CD2, CD3, CD4, CD7, CD43 and CD45. Interacts with laminin (via poly-N- acetyllactosamine). Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion. Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.

Protein type: Cell adhesion

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; cell surface; cytoplasm; extracellular region; intracellular; nucleus

Molecular Function: signal transducer activity; protein binding; protein homodimerization activity; lactose binding; galactose binding; laminin binding; galactoside binding; carbohydrate binding; glycoprotein binding

Biological Process: myoblast differentiation; heterophilic cell adhesion; plasma cell differentiation; positive regulation of I-kappaB kinase/NF-kappaB cascade; apoptosis; T cell costimulation; positive regulation of erythrocyte aggregation; signal transduction
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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