Prostaglandin D2 Synthase 21kDa (Brain) (PTGDS) ELISA Kit for Human

Name: Human PTGDS ELISA Kit; Prostaglandin D2 Synthase 21kDa (Brain); MyBioSource
Brand: MyBioSource
SKU: MBS916794
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS916794

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PTGDS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PTGDS present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

prostaglandin D2 synthase 21kDa (brain) (PTGDS), ELISA Kit

Full Product Name

Human Prostaglandin-H2 D-isomerase, PTGDS ELISA Kit

Product Gene Name

PTGDS elisa kit

Product Synonym Names

Human Prostaglandin-H2 D-isomerase (PTGDS) ELISA kit; RP11-229P13.6; L-PGDS; LPGDS; PDS; PGD2; PGDS; PGDS2; PGD2 synthase; beta-trace protein; glutathione-independent PGD synthase; lipocalin-type prostaglandin D synthase; prostaglandin D synthase; prostagla; prostaglandin D2 synthase 21kDa (brain)

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, cell culture supernates, tissue homogenates

Detection Range

0.78 ng/ml -50 ng/ml

Sensitivity

0.195 ng/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of human PTGDS. No significant cross-reactivity or interference between human PTGDS and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess

Molecular Weight

21,029 Da

OMIM

176803

3D Structure

ModBase 3D Structure for P41222

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PTGDS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PTGDS purchase

MBS916794 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the prostaglandin D2 synthase 21kDa (brain) (PTGDS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PTGDS. The ELISA analytical biochemical technique of the MBS916794 kit is based on PTGDS antibody-PTGDS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PTGDS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PTGDS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PTGDS. It may not necessarily be applicable to this product.

NCBI GI #

32171249

NCBI GeneID

5730

NCBI Accession #

NP_000945.3

UniProt Primary Accession #

P41222

UniProt Secondary Accession #

Q5SQ10; Q7M4P3; Q9UC22; Q9UCC9; Q9UCD9; B2R727

UniProt Related Accession #

P41222

GenBank Nucleotide #

NM_000954.5

NCBI Official Full Name

prostaglandin-H2 D-isomerase

NCBI Official Synonym Full Names

prostaglandin D2 synthase 21kDa (brain)

NCBI Official Symbol

PTGDS

NCBI Official Synonym Symbols

PDS; PGD2; PGDS; LPGDS; PGDS2; L-PGDS

UniProt Protein Name

Prostaglandin-H2 D-isomerase

UniProt Synonym Protein Names

Beta-trace protein; Cerebrin-28; Glutathione-independent PGD synthase; Lipocalin-type prostaglandin-D synthase; Prostaglandin-D2 synthase; PGD2 synthase; PGDS; PGDS2

Protein Family

Prostaglandin-H2 D-isomerase

UniProt Gene Name

PTGDS

UniProt Synonym Gene Names

PDS; PGD2 synthase; PGDS; PGDS2

UniProt Entry Name

PTGDS_HUMAN

NCBI Protein Information

prostaglandin-H2 D-isomerase; cerebrin-28; PGD2 synthase; beta-trace protein; prostaglandin D synthase; prostaglandin-D2 synthase; glutathione-independent PGD synthase; glutathione-independent PGD synthetase; lipocalin-type prostaglandin D synthase; lipocalin-type prostaglandin-D synthase

NCBI Summary for PTGDS

The protein encoded by this gene is a glutathione-independent prostaglandin D synthase that catalyzes the conversion of prostaglandin H2 (PGH2) to postaglandin D2 (PGD2). PGD2 functions as a neuromodulator as well as a trophic factor in the central nervous system. PGD2 is also involved in smooth muscle contraction/relaxation and is a potent inhibitor of platelet aggregation. This gene is preferentially expressed in brain. Studies with transgenic mice overexpressing this gene suggest that this gene may be also involved in the regulation of non-rapid eye movement sleep. [provided by RefSeq, Jul 2008]

UniProt Comments for PTGDS

PTGDS: Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system. Belongs to the calycin superfamily. Lipocalin family.

Protein type: Secreted, signal peptide; EC 5.3.99.2; Endoplasmic reticulum; Isomerase; Lipid Metabolism - arachidonic acid; Secreted

Chromosomal Location of Human Ortholog: 9q34.2-q34.3

Cellular Component: Golgi apparatus; extracellular space; endoplasmic reticulum membrane; nuclear membrane; rough endoplasmic reticulum; perinuclear region of cytoplasm; extracellular region

Molecular Function: retinoid binding; prostaglandin-D synthase activity; transporter activity; fatty acid binding

Biological Process: transport; cyclooxygenase pathway; response to glucocorticoid stimulus; arachidonic acid metabolic process; prostaglandin biosynthetic process; regulation of circadian sleep/wake cycle, sleep

Research Articles on PTGDS

1. Lipocalin-type prostaglandin D2 (PGD2) synthase (L-PGDS) interacts intracellularly with the G protein-coupled receptor DP1 in an agonist-independent manner.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PTGDS elisa kit

Reference Product	PubMed Publications
PTGFR elisa kit	>1 publications with PTGDS and PTGFR
PTGER2 elisa kit	>1 publications with PTGDS and PTGER2
PTGER1 elisa kit	>1 publications with PTGDS and PTGER1

Pathways associated with PTGDS elisa kit

Products by Pathway	Pathway Diagram
Arachidonic Acid Metabolism Pathway antibodies	Arachidonic Acid Metabolism Pathway Diagram
Arachidonic Acid Metabolism Pathway antibodies	Arachidonic Acid Metabolism Pathway Diagram
Eicosanoid Synthesis Pathway antibodies	Eicosanoid Synthesis Pathway Diagram
Prostaglandin Synthesis And Regulation Pathway antibodies	Prostaglandin Synthesis And Regulation Pathway Diagram
Prostanoid Biosynthesis Pathway antibodies	Prostanoid Biosynthesis Pathway Diagram

Diseases associated with PTGDS elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>4 publications with PTGDS and Neoplasms
Brain Diseases Antibodies	>2 publications with PTGDS and Brain Diseases
Mood Disorders Antibodies	>1 publications with PTGDS and Mood Disorders
Breast Neoplasms Antibodies	>1 publications with PTGDS and Breast Neoplasms
Depressive Disorder Antibodies	>1 publications with PTGDS and Depressive Disorder
Skin Diseases Antibodies	>1 publications with PTGDS and Skin Diseases
Pain Antibodies	>1 publications with PTGDS and Pain
Hyperplasia Antibodies	>1 publications with PTGDS and Hyperplasia
Drug Toxicity Antibodies	>1 publications with PTGDS and Drug Toxicity
Nervous System Malformations Antibodies	>1 publications with PTGDS and Nervous System Malformations

Organs/Tissues associated with PTGDS elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>7 publications with PTGDS and Brain
Blood Antibodies	>5 publications with PTGDS and Blood
Testis Antibodies	>4 publications with PTGDS and Testis
Embryonic Tissue Antibodies	>2 publications with PTGDS and Embryonic Tissue
Adipose Tissue Antibodies	>2 publications with PTGDS and Adipose Tissue
Ovary Antibodies	>2 publications with PTGDS and Ovary
Connective Tissue Antibodies	>2 publications with PTGDS and Connective Tissue
Eye Antibodies	>2 publications with PTGDS and Eye
Nerve Antibodies	>1 publications with PTGDS and Nerve
Prostate Antibodies	>1 publications with PTGDS and Prostate