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FLT3 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS859699

MBS859699 is an antibody targeting FLT3. Also known as FLT3 (Phospho-Tyr599) Antibody. Reactive in Human, Mouse. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
FLT3, Antibody
Full Product Name
FLT3 (Phospho-Tyr599) Antibody
Product Gene Name
Product Synonym Names
CD135; EC 2.7.10.1; FL cytokine receptor precursor; FLK-2; FLT-3; FLT3; Fetal liver kinase 2; STK-1; STK1; Stem cell tyrosine kinase 1; flk-2; kinase Flt3
Species Reactivity
Human, Mouse
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificity
FLT3 (Phospho-Tyr599) antibody detects endogenous levels of FLT3 only when phosphorylated at tyrosine 599.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
108,378 Da
OMIM
136351
3D Structure
ModBase 3D Structure for P36888
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human FLT3 around the phosphorylation site of tyrosine 599 (Y-E-YP-D-L).
All Sites
Human: Tyr599
Mouse: Tyr600
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-FLT3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-FLT3 antibody
WB: 1:500-1:1000
ELISA: 1:1000

Western Blot (WB) of anti-FLT3 antibody

Western blot analysis of extracts from COS7 cells, treated with EGF (200ng/ml, 30mins), using FLT3 (Phospho-Tyr599) antibody.
Anti-FLT3 Antibody – Cat# MBS859699

Product References and Citations for anti-FLT3 antibody

Small D., Proc. Natl. Acad. Sci. U.S.A. 91:459-463(1994). Rosnet O., Blood 82:1110-1119(1993). Greenman C., Nature 446:153-158(2007).

Other Notes

Small volumes of anti-FLT3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for FLT3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
receptor-type tyrosine-protein kinase FLT3
NCBI Official Synonym Full Names
fms related tyrosine kinase 3
NCBI Official Symbol
FLT3
NCBI Official Synonym Symbols
FLK2; STK1; CD135; FLK-2
UniProt Protein Name
Receptor-type tyrosine-protein kinase FLT3
UniProt Synonym Protein Names
FL cytokine receptor; Fetal liver kinase-2; FLK-2; Fms-like tyrosine kinase 3; FLT-3; Stem cell tyrosine kinase 1; STK-1; CD_antigen: CD135
UniProt Gene Name
FLT3
UniProt Synonym Gene Names
CD135; FLK2; STK1; FLK-2; FLT-3; STK-1
NCBI Protein Information
receptor-type tyrosine-protein kinase FLT3

NCBI Summary for FLT3

This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia. [provided by RefSeq, Jan 2015]
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UniProt Comments for FLT3

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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