Integrin, Alpha 4 (Antigen CD49D, Alpha 4 Subunit of VLA-4 Receptor) (ITGA4) ELISA Kit for Human

Name: Human ITGA4 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS918697
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS918697

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ITGA4/CD49D has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ITGA4/CD49D present is bound… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) (ITGA4), ELISA Kit

Full Product Name

Human Integrin alpha-4, ITGA4/CD49D ELISA Kit

Product Gene Name

ITGA4 elisa kit

Product Synonym Names

Human Integrin alpha-4 (ITGA4/CD49D) ELISA kit; CD49D; IA4; MGC90518; 269C wild type; antigen CD49D; alpha-4 subunit of VLA-4 receptor; integrin alpha 4; integrin alpha-4 subunit; very late activation protein 4 receptor; alpha 4 subunit; integrin; alpha 4 (antigen CD49D; alpha 4 subunit of VLA-4 receptor)

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates

Detection Range

0.312 ng/ml-20 ng/ml.

Sensitivity

The minimum detectable dose of human ITGA4/CD49D is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Specificity

This assay has high sensitivity and excellent specificity for detection of human ITGA4/CD49D. No significant cross-reactivity or interference between human ITGA4/CD49D and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

114,900 Da

OMIM

192975

3D Structure

ModBase 3D Structure for P13612

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of ITGA4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ITGA4 purchase

MBS918697 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) (ITGA4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ITGA4. The ELISA analytical biochemical technique of the MBS918697 kit is based on ITGA4 antibody-ITGA4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ITGA4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ITGA4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ITGA4. It may not necessarily be applicable to this product.

NCBI GI #

67191027

NCBI GeneID

3676

NCBI Accession #

NP_000876.3

UniProt Primary Accession #

P13612

UniProt Secondary Accession #

D3DPG4

UniProt Related Accession #

P13612; AAB25486

GenBank Nucleotide #

NM_000885.4

NCBI Official Full Name

integrin alpha-4

NCBI Official Synonym Full Names

integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)

NCBI Official Symbol

ITGA4

NCBI Official Synonym Symbols

IA4; CD49D

UniProt Protein Name

Integrin alpha-4

UniProt Synonym Protein Names

CD49 antigen-like family member D; Integrin alpha-IV; VLA-4 subunit alpha; CD_antigen: CD49d

Protein Family

Integrin

UniProt Gene Name

ITGA4

UniProt Synonym Gene Names

CD49D

UniProt Entry Name

ITA4_HUMAN

NCBI Protein Information

integrin alpha-4; 269C wild type; integrin alpha 4; integrin alpha-IV; VLA-4 subunit alpha; integrin alpha-4 subunit; CD49 antigen-like family member D; antigen CD49D, alpha-4 subunit of VLA-4 receptor; very late activation protein 4 receptor, alpha 4 subunit

NCBI Summary for ITGA4

The product of this gene belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an alpha 4 chain. Unlike other integrin alpha chains, alpha 4 neither contains an I-domain, nor undergoes disulfide-linked cleavage. Alpha 4 chain associates with either beta 1 chain or beta 7 chain. [provided by RefSeq, Jul 2008]

UniProt Comments for ITGA4

ITGA4: Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha- 4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha- 4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. Heterodimer of an alpha and a beta subunit. The alpha subunit can sometimes be cleaved into two non-covalently associated fragments. Alpha-4 associates with either beta-1 or beta-7. Alpha-4 interacts with PXN, LPXN, and TGFB1I1/HIC5. Interacts with CSPG4 through CSPG4 chondroitin sulfate glycosaminoglycan. Belongs to the integrin alpha chain family.

Protein type: Receptor, misc.; Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 2q31.3

Cellular Component: focal adhesion; cell surface; membrane; plasma membrane; intercellular junction; external side of plasma membrane

Molecular Function: protein binding; fibronectin binding; metal ion binding; cell adhesion molecule binding

Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; regulation of immune response; heart development; cell-matrix adhesion; receptor clustering; heterophilic cell adhesion; heterotypic cell-cell adhesion; leukocyte adhesion; B cell differentiation; blood vessel remodeling; leukocyte tethering or rolling; blood coagulation; leukocyte migration

Research Articles on ITGA4

1. Disruption of the hydrophobic contacts induces the active conformation of integrin alpha 4 beta 7.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ITGA4 elisa kit

Reference Product	PubMed Publications
ITGB1 elisa kit	>7 publications with ITGA4 and ITGB1
ITGB7 elisa kit	>2 publications with ITGA4 and ITGB7
FN1 elisa kit	>1 publications with ITGA4 and FN1
ADAM28 elisa kit	>1 publications with ITGA4 and ADAM28

Pathways associated with ITGA4 elisa kit

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies	Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies	Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Surface Interactions At The Vascular Wall Pathway antibodies	Cell Surface Interactions At The Vascular Wall Pathway Diagram
Dilated Cardiomyopathy Pathway antibodies	Dilated Cardiomyopathy Pathway Diagram
Dilated Cardiomyopathy Pathway antibodies	Dilated Cardiomyopathy Pathway Diagram
ECM-receptor Interaction Pathway antibodies	ECM-receptor Interaction Pathway Diagram
ECM-receptor Interaction Pathway antibodies	ECM-receptor Interaction Pathway Diagram

Diseases associated with ITGA4 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>10 publications with ITGA4 and Neoplasms
Mental Disorders Antibodies	>5 publications with ITGA4 and Mental Disorders
Intestinal Diseases Antibodies	>5 publications with ITGA4 and Intestinal Diseases
Autistic Disorder Antibodies	>4 publications with ITGA4 and Autistic Disorder
Neoplasms, Experimental Antibodies	>2 publications with ITGA4 and Neoplasms, Experimental
Inflammation Antibodies	>2 publications with ITGA4 and Inflammation
Necrosis Antibodies	>2 publications with ITGA4 and Necrosis
Disease Models, Animal Antibodies	>1 publications with ITGA4 and Disease Models, Animal
Melanoma Antibodies	>1 publications with ITGA4 and Melanoma
Kidney Diseases Antibodies	>1 publications with ITGA4 and Kidney Diseases

Organs/Tissues associated with ITGA4 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>10 publications with ITGA4 and Blood
Vascular Antibodies	>3 publications with ITGA4 and Vascular
Brain Antibodies	>3 publications with ITGA4 and Brain
Lymph Antibodies	>3 publications with ITGA4 and Lymph
Lymph Node Antibodies	>3 publications with ITGA4 and Lymph Node
Embryonic Tissue Antibodies	>2 publications with ITGA4 and Embryonic Tissue
Bone Antibodies	>2 publications with ITGA4 and Bone
Bone Marrow Antibodies	>2 publications with ITGA4 and Bone Marrow
Uterus Antibodies	>2 publications with ITGA4 and Uterus
Placenta Antibodies	>2 publications with ITGA4 and Placenta