Pro-Melanin-Concentrating Hormone (PMCH) ELISA Kit for Mouse

Name: Mouse PMCH ELISA Kit; Pro-Melanin-Concentrating Hormone; MyBioSource
Brand: MyBioSource
SKU: MBS918862
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS918862

Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PMCH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PMCH present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

pro-melanin-concentrating hormone (PMCH), ELISA Kit

Full Product Name

Mouse pro-melanin-concentrating hormone, PMCH ELISA Kit

Product Gene Name

PMCH elisa kit

Product Synonym Names

Mouse pro-melanin-concentrating hormone (PMCH) ELISA kit; MCH; ; pro-melanin-concentrating hormone

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates.

Detection Range

6.25 pg/ml -400 pg/ml.

Sensitivity

1.56 pg/ml.

Specificity

This assay has high sensitivity and excellent specificity for detection of mouse PMCH. No significant cross-reactivity or interference between mouse PMCH and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

18,516 Da

3D Structure

ModBase 3D Structure for P56942

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PMCH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PMCH purchase

MBS918862 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the pro-melanin-concentrating hormone (PMCH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PMCH. The ELISA analytical biochemical technique of the MBS918862 kit is based on PMCH antibody-PMCH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PMCH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PMCH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PMCH. It may not necessarily be applicable to this product.

NCBI GI #

71361678

NCBI GeneID

110312

NCBI Accession #

NP_084247.1

UniProt Primary Accession #

P56942

UniProt Secondary Accession #

Q9D220

UniProt Related Accession #

P56942

GenBank Nucleotide #

NM_029971.2

NCBI Official Full Name

pro-MCH preproprotein

NCBI Official Synonym Full Names

pro-melanin-concentrating hormone

NCBI Official Symbol

Pmch

NCBI Official Synonym Symbols

A230109K23Rik

UniProt Protein Name

Pro-MCH

Protein Family

Pro-MCH

UniProt Gene Name

Pmch

UniProt Synonym Gene Names

Mch; NGE; Neuropeptide G-E; NEI; Neuropeptide E-I; MCH

UniProt Entry Name

MCH_MOUSE

NCBI Protein Information

pro-MCH; neuropeptide-glycine-glutamic acid (NGE); neuropeptide-glutamic acid-isoleucine (NEI)

NCBI Summary for PMCH

This gene encodes a preproprotein that is cleaved to produce three mature peptides: melanin concentrating hormone, neuropeptide-glutamic acid-isoleucine (NEI), and neuropeptide-glycine-glutamic acid (NGE). The proprotein is processed differently depending on the tissue where it is expressed. Melanin concentrating hormone is involved in the regulation of food intake, energy homeostasis, and sleep-wake behavior. Disruption of this gene is associated with resistance to diet-induced obesity. [provided by RefSeq, May 2013]

UniProt Comments for PMCH

PMCH: MCH may act as a neurotransmitter or neuromodulator in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal. May also have a role in spermatocyte differentiation. Belongs to the melanin-concentrating hormone family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; cell; extracellular region; nucleus; cytosol

Molecular Function: type 1 melanin-concentrating hormone receptor binding; melanin-concentrating hormone activity; hormone activity; receptor binding

Biological Process: synaptic transmission; elevation of cytosolic calcium ion concentration; positive regulation of circadian sleep/wake cycle, REM sleep; regulation of heart rate; neuropeptide signaling pathway; drinking behavior; feeding behavior; response to cold; negative regulation of blood pressure; glucose homeostasis; negative regulation of synaptic transmission, dopaminergic; regulation of neuronal synaptic plasticity

Research Articles on PMCH

1. acute activation of MCH neurons is sufficient, but not necessary, to trigger the transition from NREM to REM sleep and MCH neurons also play a role in the initiation and maintenance of NREM sleep.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with PMCH elisa kit

Products by Pathway	Pathway Diagram
G Alpha (i) Signalling Events Pathway antibodies	G Alpha (i) Signalling Events Pathway Diagram
G Alpha (q) Signalling Events Pathway antibodies	G Alpha (q) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By GPCR Pathway antibodies	Signaling By GPCR Pathway Diagram

Organs/Tissues associated with PMCH elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>9 publications with PMCH and Brain
Bladder Antibodies	>1 publications with PMCH and Bladder